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[185143_at]

Description Y25C1A.2 /REP_DB=WormBase Gene ID /WP=CE21469 /TR=Q9TYL1 /GB=AAD12834.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 0      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 11
(-3)
A 		25.7
(1.4)
A 		31.2
(3)
A 		30.4
(2.8)
A 		20
B 12.4
(3)
A 		12.1
(2.7)
A 		11.6
(2.2)
A 		6.1
(-3)
A 		6
C 21.2
(0.5)
A 		7.1
(-3)
A 		17.8
(-0.3)
A 		31
(3)
A 		24
avr 14.9
(-3) 		15
(-2.9) 		20.2
(1.2) 		22.5
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 14.2
(-0.8)
A 		1.9
(-3)
A 		9.3
(-1.7)
A 		4.8
(-2.5)
A 		35.7
(3)
A 		8.6
(-1.8)
A 		34
B 10.9
(2.1)
A 		8.9
(-0.4)
A 		11.6
(3)
A 		7.3
(-2.5)
A 		9.6
(0.4)
A 		6.9
(-3)
A 		5
C 7.9
(-0.4)
A 		8
(-0.4)
A 		3.4
(-2.8)
A 		14.2
(3)
A 		9.1
(0.2)
A 		3.1
(-3)
A 		11
avr 11
(-0.6) 		6.3
(-2.9) 		8.1
(-2) 		8.8
(-1.7) 		18.1
(3) 		6.2
(-3) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 4
(-3)
A 		5.6
(-0.7)
A 		8.2
(3)
A 		4.5
(-2.3)
A 		4
B 4
(3)
A 		3
(0.8)
A 		2.7
(0.1)
A 		1.3
(-3)
A 		3
C 7
(3)
A 		2
(-3)
A 		6.2
(2)
A 		5.2
(0.8)
A 		5
avr 5
(1.1) 		3.5
(-3) 		5.7
(3) 		3.7
(-2.5) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 2.6
(-1.9)
A 		0.7
(-3)
A 		2
(-2.2)
A 		1.2
(-2.7)
A 		10.8
(3)
A 		2.1
(-2.2)
A 		10
B 3.8
(2.7)
A 		2.9
(0)
A 		2.8
(-0.3)
A 		1.9
(-3)
A 		3.9
(3)
A 		2.6
(-0.9)
A 		2
C 2.9
(3)
A 		2.5
(1.8)
A 		0.9
(-3)
A 		2.9
(3)
A 		2.9
(3)
A 		1
(-2.7)
A 		2
avr 3.1
(-1.2) 		2
(-2.9) 		1.9
(-3) 		2
(-2.9) 		5.9
(3) 		1.9
(-3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185143_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5323
Sequence Type Exemplar sequence
Representative Public ID Y25C1A.2 NCBI
Target Description Y25C1A.2 /REP_DB=WormBase Gene ID /WP=CE21469 /TR=Q9TYL1 /GB=AAD12834.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062048(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062048 NCBI Caenorhabditis elegans Y25C1A.2 (Y25C1A.2) mRNA, complete cds. 11/11 None
SNAP00000041888 ENSEMBL cdna:SNAP chromosome:CEL140:II:3110208:3113251:1 10/11 None
GENEFINDER00000041890 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3112615:3113198:1 11/11 None
Y25C1A.2 ENSEMBL cdna:known chromosome:CEL140:II:3112615:3113198:1 gene:Y25C1A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3112613-3113197(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y25C1A.2
Entrez Gene 189535 Entrez gene
SwissProt Q9TYL1 EMBL-EBI
WormBase CE21469 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494449 Y25C1A.2 [Caenorhabditis elegans] gb|AAD12834.1| Hypothetical protein Y25C1A.2 [Caenorhabditis elegans] 6.0E-84
blast NP_494449 Y25C1A.2 [Caenorhabditis elegans] gb|AAD12834.1| Hypothetical protein Y25C1A.2 [Caenorhabditis elegans] 3.0E-77
blast AAB66208 Hypothetical protein F45C12.9 [Caenorhabditis elegans] ref|NP_494051.1| F45C12.9 [Caenorhabditis elegans] 2.0E-24
blast AAB66208 Hypothetical protein F45C12.9 [Caenorhabditis elegans] ref|NP_494051.1| F45C12.9 [Caenorhabditis elegans] 3.0E-21
blast AAG00001 Hypothetical protein R03H10.5 [Caenorhabditis elegans] ref|NP_494732.1| R03H10.5 [Caenorhabditis elegans] 7.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 7.3E-10
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 4.1E-10
Sequence
Target Sequence 
>C. ELEGANS:185143_AT
tcgtcgtcaccgcagaacatggatgtgcagattcctgctccatgtgcccagatattcaaa
gtatttttagtaacaagaccggtagtacaatcctgtactccgaaggacctggatgcgtta
gaaacttgacatgtactgtgaaaggaaccttcacatcaatttactcggcattctccgtct
cagatgttccacggcctgagaatattgctgatcatcctgaggttgatacgatgataatct
acgcgacaagcgttggtggttccggtggttccggtggtgttggagttgtgatcgatcttt
actccttctatggaatagtttgcgaagatgcccagtggtacgctaccaagtatccatatg
gaatttcctacacagattctgatggagggtggatgcttatcggcttcaatggggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGTCGTCACCGCAGAACATGGATG 432 603 50 Antisense
TGGATGTGCAGATTCCTGCTCCATG 239 549 69 Antisense
TGCTCCATGTGCCCAGATATTCAAA 139 581 85 Antisense
ACAAGACCGGTAGTACAATCCTGTA 577 111 122 Antisense
GTACTCCGAAGGACCTGGATGCGTT 258 459 144 Antisense
ACCTTCACATCAATTTACTCGGCAT 37 87 196 Antisense
ATTCTCCGTCTCAGATGTTCCACGG 498 9 219 Antisense
GTTCCACGGCCTGAGAATATTGCTG 460 439 235 Antisense
AGTTGTGATCGATCTTTACTCCTTC 543 65 333 Antisense
CGAAGATGCCCAGTGGTACGCTACC 555 251 372 Antisense
GATGCTTATCGGCTTCAATGGGGAA 673 413 441 Antisense
Sequence Source Affymetrix Proprietary Database