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[185142_at]

Description E02C12.8 /REP_DB=WormBase Gene ID /WP=CE04317 /TR=Q19032 /GB=AAA83465.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 30      4grp_total > 56      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 7.4
(-2.4)
A 		12.2
(-1.6)
A 		36.8
(3)
A 		4.4
(-3)
A 		32
B 37.7
(2.3)
A 		42.5
(3)
A 		27.6
(0.7)
A 		2.8
(-3)
A 		40
C 11.9
(-2.9)
A 		11.5
(-3)
A 		44.7
(2.5)
M 		47.4
(3)
A 		36
avr 19
(-2.7) 		22.1
(-1.7) 		36.4
(3) 		18.2
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 23.4
(1.4)
A 		5.9
(-3)
A 		29.7
(3)
A 		14.6
(-0.8)
A 		11.2
(-1.7)
A 		20.7
(0.7)
A 		24
B 20.4
(1.3)
A 		27
(3)
A 		19.1
(0.9)
A 		24.6
(2.4)
A 		26.6
(2.9)
A 		3.9
(-3)
A 		23
C 10.1
(-1.8)
A 		42.1
(3)
A 		8.8
(-2)
A 		22.7
(0.1)
A 		1.8
(-3)
A 		8.9
(-1.9)
A 		40
avr 18
(-0) 		25
(3) 		19.2
(0.5) 		20.6
(1.1) 		13.2
(-2.1) 		11.2
(-3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 2.7
(-1.6)
A 		2.7
(-1.6)
A 		9.6
(3)
A 		0.6
(-3)
A 		9
B 12
(3)
A 		10.5
(2.2)
A 		6.4
(0.1)
A 		0.6
(-3)
A 		11
C 4
(-2.7)
A 		3.3
(-3)
A 		15.6
(3)
M 		8
(-0.7)
A 		12
avr 6.2
(-0.5) 		5.5
(-1.1) 		10.5
(3) 		3.1
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 4.3
(-0)
A 		2.1
(-3)
A 		6.5
(3)
A 		3.7
(-0.8)
A 		3.4
(-1.2)
A 		5
(1)
A 		4
B 7.1
(0.6)
A 		9
(1.8)
A 		4.6
(-1)
A 		6.5
(0.2)
A 		10.8
(3)
A 		1.5
(-3)
A 		9
C 3.8
(-1.4)
A 		12.9
(3)
A 		2.2
(-2.2)
A 		4.6
(-1)
A 		0.6
(-3)
A 		3
(-1.8)
A 		12
avr 5.1
(-0.6) 		8
(3) 		4.4
(-1.5) 		4.9
(-0.9) 		4.9
(-0.9) 		3.2
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185142_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11531
Sequence Type Exemplar sequence
Representative Public ID E02C12.8 NCBI
Target Description E02C12.8 /REP_DB=WormBase Gene ID /WP=CE04317 /TR=Q19032 /GB=AAA83465.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073026(11), NM_001028591(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073026 NCBI Caenorhabditis elegans E02C12.8a (E02C12.8) mRNA, complete cds. 11/11 A 
NM_001028591 NCBI Caenorhabditis elegans E02C12.8c (E02C12.8) mRNA, complete cds. 11/11 None
SNAP00000032007 ENSEMBL cdna:SNAP chromosome:CEL140:V:9375233:9376134:-1 11/11 None
GENEFINDER00000032017 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9374246:9376134:-1 9/11 None
E02C12.8a ENSEMBL cdna:known chromosome:CEL140:V:9374224:9376142:-1 gene:E02C12.8 11/11 A 
E02C12.8c ENSEMBL cdna:known chromosome:CEL140:V:9374246:9376134:-1 gene:E02C12.8 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_073027 7/11 Cross Hyb Matching Probes None
E02C12.8b 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179320 3 2 NM_001028591 185142_at (11), 185273_at (11)
NM_073026 185142_at (11), 185273_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9375232-9376134(-) 100.0 100.0
Public Domain and Genome References
Ensembl E02C12.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN84829 Hypothetical protein E02C12.8a [Caenorhabditis elegans] ref|NP_505427.2| E02C12.8a [Caenorhabditis elegans] 0.0
blast AAP68946 Hypothetical protein E02C12.8c [Caenorhabditis elegans] ref|NP_001023762.1| E02C12.8c [Caenorhabditis elegans] 0.0
blast AAP68946 Hypothetical protein E02C12.8c [Caenorhabditis elegans] ref|NP_001023762.1| E02C12.8c [Caenorhabditis elegans] 1.0E-150
blast AAA83463 Hypothetical protein E02C12.6 [Caenorhabditis elegans] ref|NP_505426.2| E02C12.6 [Caenorhabditis elegans] 1.0E-145
Sequence
Target Sequence 
>C. ELEGANS:185142_AT
cgctttacgaacctgccgatggaattcttgaaacccatgtgacatgggaagacgtcgaga
aggaccttcaacaaagtcttggaacgcgagctacatttggagaaaataagagagcgacaa
atatcagcgatttgaaaggtttcatgtcaagaatcgcctgccttgaacctgattggcaaa
acattgaggaaggaaaggaacttccatcaaaatttgcgttgaagataagctctcagctgg
ctcttgttgctttatcgaaaatcatgaattttgaagaaggtgccggattttctgaagaaa
aattgaagaagttttcgagtttaacaaaagaatgtcataatagagaggtcgacgcatata
aggttttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCTTTACGAACCTGCCGATGGAAT 200 245 17 Antisense
GAAGGACCTTCAACAAAGTCTTGGA 127 333 75 Antisense
AAAGTCTTGGAACGCGAGCTACATT 31 123 89 Antisense
AAGAGAGCGACAAATATCAGCGATT 649 153 124 Antisense
GTTTCATGTCAAGAATCGCCTGCCT 166 451 155 Antisense
TCGCCTGCCTTGAACCTGATTGGCA 358 599 170 Antisense
AACTTCCATCAAAATTTGCGTTGAA 37 139 215 Antisense
GAAGATAAGCTCTCAGCTGGCTCTT 38 339 237 Antisense
TCAGCTGGCTCTTGTTGCTTTATCG 334 625 249 Antisense
TTGAAGAAGGTGCCGGATTTTCTGA 295 701 287 Antisense
GAGGTCGACGCATATAAGGTTTTGA 469 401 361 Antisense
Sequence Source Affymetrix Proprietary Database