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[185140_at]

Description H25P19.1 /REP_DB=WormBase Gene ID /WP=CE20998 /TR=Q9UB02 /GB=AAD14770.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 69      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_total > 79      4grp_total > 61      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 179.4
(-1)
P 		173.6
(-3)
P 		186.7
(1.6)
P 		190.7
(3)
P 		17
B 171.4
(3)
P 		148.5
(1.1)
P 		150.4
(1.2)
P 		99.9
(-3)
P 		72
C 173.2
(3)
P 		169.9
(2.2)
P 		155.5
(-1.1)
P 		147
(-3)
P 		26
avr 174.7
(3) 		164
(0.8) 		164.2
(0.8) 		145.9
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 147
1 2 3 4 5 6 range
A 176.9
(1.1)
P 		174
(1)
P 		86.3
(-3)
P 		123.2
(-1.3)
P 		175.2
(1.1)
P 		217.6
(3)
P 		131
B 161.1
(-0.7)
P 		193.3
(0.9)
P 		116.2
(-3)
P 		119.2
(-2.8)
P 		151
(-1.2)
P 		233.4
(3)
P 		117
C 136.9
(-1.4)
P 		175.2
(1.5)
P 		168.9
(1)
P 		116.6
(-3)
M 		168.9
(1)
P 		195
(3)
P 		78
avr 158.3
(-0.6) 		180.8
(0.8) 		123.8
(-2.7) 		119.7
(-3) 		165
(-0.2) 		215.3
(3) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 65.3
(3)
P 		37.7
(-1.4)
P 		48.8
(0.4)
P 		27.9
(-3)
P 		37
B 54.6
(3)
P 		36.7
(-0.2)
P 		34.8
(-0.6)
P 		21.3
(-3)
P 		33
C 57.3
(3)
P 		48.9
(1.4)
P 		54.2
(2.4)
P 		24.8
(-3)
P 		33
avr 59.1
(3) 		41.1
(-0.1) 		45.9
(0.7) 		24.7
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 32.6
(-1.1)
P 		61.9
(3)
P 		18.8
(-3)
P 		31.3
(-1.3)
P 		52.9
(1.7)
P 		53
(1.8)
P 		43
B 56.1
(-0.2)
P 		64.3
(0.7)
P 		28.2
(-3)
P 		31.6
(-2.7)
P 		61.4
(0.4)
P 		87
(3)
P 		59
C 50.7
(0.9)
P 		53.5
(1.3)
P 		43
(-0.2)
P 		23.5
(-3)
M 		54.6
(1.5)
P 		65.3
(3)
P 		42
avr 46.5
(-0.3) 		59.9
(1.7) 		30
(-2.8) 		28.8
(-3) 		56.3
(1.2) 		68.4
(3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185140_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15270
Sequence Type Exemplar sequence
Representative Public ID H25P19.1 NCBI
Target Description H25P19.1 /REP_DB=WormBase Gene ID /WP=CE20998 /TR=Q9UB02 /GB=AAD14770.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070894(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070894 NCBI Caenorhabditis elegans H25P19.1 (H25P19.1) mRNA, complete cds. 11/11 None
H25P19.1 ENSEMBL cdna:known chromosome:CEL140:V:687545:689829:1 gene:H25P19.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:687544-688298(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H25P19.1
Entrez Gene 186785 Entrez gene
SwissProt Q9UB02 EMBL-EBI
WormBase CE31026 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD14770 Hypothetical protein H25P19.1 [Caenorhabditis elegans] ref|NP_503295.2| H25P19.1 [Caenorhabditis elegans] 1.0E-165
blast NP_503765 T03D3.5 [Caenorhabditis elegans] gb|AAG24188.3| Hypothetical protein T03D3.5 [Caenorhabditis elegans] 3.0E-44
blast CAE62230 Hypothetical protein CBG06282 [Caenorhabditis briggsae] 5.0E-43
Sequence
Target Sequence 
>C. ELEGANS:185140_AT
atctaccgcagaaattccggattttcgaacaagcgccgtctctgaaagggataatgaaac
aattttaatgcttccaccagtaggaaatgtcgaagagcacgatattgagcaatttaagcg
agaaacatgtcatttgccagaggatgtggcattccgaattctctcgttctacgaatatga
ttttgaaactgcgacacgtgtcgccttgcaaatggatgctccggatttgatgccggattt
tgccaagaaaattatgatcgcgaatgctccgtttgaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCTACCGCAGAAATTCCGGATTTT 65 33 147 Antisense
TCCGGATTTTCGAACAAGCGCCGTC 367 593 162 Antisense
ACAAGCGCCGTCTCTGAAAGGGATA 490 111 175 Antisense
AACAATTTTAATGCTTCCACCAGTA 235 131 204 Antisense
GCGAGAAACATGTCATTTGCCAGAG 548 289 264 Antisense
AGGATGTGGCATTCCGAATTCTCTC 310 57 287 Antisense
TCCGAATTCTCTCGTTCTACGAATA 217 593 299 Antisense
GAAACTGCGACACGTGTCGCCTTGC 470 357 331 Antisense
TCGCCTTGCAAATGGATGCTCCGGA 350 599 347 Antisense
GGATTTGATGCCGGATTTTGCCAAG 33 511 369 Antisense
TGATCGCGAATGCTCCGTTTGAAAG 320 565 401 Antisense
Sequence Source Affymetrix Proprietary Database