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[185128_at]

Description Y53G8B.3 /REP_DB=WormBase Gene ID /WP=CE25429 /TR=Q9N4S2 /GB=AAF60756.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 39      4grp_total > 25      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 3.4
(-3)
A 		13
(-2)
A 		25.5
(-0.6)
A 		58.9
(3)
A 		56
B 20.2
(-1.6)
A 		61.2
(3)
A 		28
(-0.7)
A 		7.4
(-3)
A 		54
C 28.5
(-2.5)
A 		24.3
(-3)
A 		25.9
(-2.8)
M 		79.7
(3)
P 		55
avr 17.4
(-3) 		32.8
(-0) 		26.5
(-1.3) 		48.7
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 63
(3)
A 		16.1
(-1.7)
A 		41.8
(0.9)
A 		2.6
(-3)
A 		43.2
(1)
A 		36.5
(0.4)
A 		60
B 13.5
(-1.4)
A 		39.2
(3)
A 		3.9
(-3)
A 		24.4
(0.5)
A 		28.4
(1.2)
A 		25.2
(0.6)
P 		35
C 25.2
(0.4)
A 		34
(1.8)
A 		11.2
(-1.8)
A 		3.5
(-3)
A 		30
(1.1)
A 		41.9
(3)
P 		38
avr 33.9
(2.9) 		29.8
(1.8) 		19
(-0.8) 		10.2
(-3) 		33.9
(2.9) 		34.5
(3) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 1.2
(-3)
A 		2.8
(-1.7)
A 		6.7
(1.5)
A 		8.6
(3)
A 		7
B 6.4
(-0.9)
A 		15.1
(3)
A 		6.5
(-0.8)
A 		1.6
(-3)
A 		14
C 9.4
(-0.8)
A 		7
(-3)
A 		9
(-1.1)
M 		13.4
(3)
P 		6
avr 5.7
(-3) 		8.3
(3) 		7.4
(0.9) 		7.9
(2.1) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 11.6
(2.3)
A 		5.7
(-0.6)
A 		9.1
(1.1)
A 		0.7
(-3)
A 		13
(3)
A 		8.9
(1)
A 		12
B 4.7
(-1.1)
A 		13.1
(3)
A 		0.9
(-3)
A 		6.5
(-0.2)
A 		11.5
(2.2)
A 		9.4
(1.2)
P 		12
C 9.4
(0.9)
A 		10.4
(1.4)
A 		2.9
(-2)
A 		0.7
(-3)
A 		9.7
(1.1)
A 		14
(3)
P 		13
avr 8.6
(1.1) 		9.7
(1.8) 		4.3
(-1.8) 		2.6
(-3) 		11.4
(3) 		10.8
(2.6) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185128_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7284
Sequence Type Exemplar sequence
Representative Public ID Y53G8B.3 NCBI
Target Description Y53G8B.3 /REP_DB=WormBase Gene ID /WP=CE25429 /TR=Q9N4S2 /GB=AAF60756.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065259(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065259 NCBI Caenorhabditis elegans Y53G8B.3 (Y53G8B.3) mRNA, complete cds. 11/11 None
GENEFINDER00000030868 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3105658:3107528:-1 11/11 None
Y53G8B.3 ENSEMBL cdna:known chromosome:CEL140:III:3105658:3108692:-1 gene:Y53G8B.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030866 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3105657-3110765(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8B.3
Entrez Gene 190244 Entrez gene
SwissProt Q9N4S2 EMBL-EBI
WormBase CE30041 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL32253 Hypothetical protein Y53G8B.3 [Caenorhabditis elegans] ref|NP_497660.2| Y53G8B.3 [Caenorhabditis elegans] 1.0E-154
blast AAL32253 Hypothetical protein Y53G8B.3 [Caenorhabditis elegans] ref|NP_497660.2| Y53G8B.3 [Caenorhabditis elegans] 3.0E-93
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497660.2 6 21-43,58-80,143-165,180-202,215-237,247-264
Y53G8B.3 6 21-43,58-80,143-165,180-202,215-237,247-264
GENEFINDER00000030868 4 89-111,126-148,161-183,193-210
Sequence
Target Sequence 
>C. ELEGANS:185128_AT
gaaggagccgtgaataccatctacagatgcttcaaaagtaccgcccgtcttccacaattt
gtgatgcgctacttgtcactgctcattcattgtagtttgatgctttttacacatttgctt
ttatttaattcccgccagattgccttcctggtcccgccttacgttgcatttgcccagatt
tttgcattttcagtgattagtttcaagaatttgtttgtggtatttgcgacaataagcatg
cggagacgtggatttttgtaccgaattcgaaaatatttgaagctttattatcaattcctt
cttcccggcttacttttgggaatgatcctctacgacgtctaccacaacggaactcggctc
gtaaaactgactctggctccagtggtccttgtcgcgttcgcccaacttttgagatacggc
tttaactcaaaaatatcgaatgcttggaaaccgacacagaaattcaaacccactttcaat
caggatttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGAGCCGTGAATACCATCTACA 95 333 802 Antisense
TTGTGATGCGCTACTTGTCACTGCT 188 707 860 Antisense
GTCACTGCTCATTCATTGTAGTTTG 575 465 876 Antisense
CCTTACGTTGCATTTGCCCAGATTT 402 267 958 Antisense
GGAGACGTGGATTTTTGTACCGAAT 98 515 1043 Antisense
TTTATTATCAATTCCTTCTTCCCGG 534 673 1085 Antisense
TTGGGAATGATCCTCTACGACGTCT 157 705 1117 Antisense
CTACCACAACGGAACTCGGCTCGTA 24 225 1140 Antisense
GGCTCGTAAAACTGACTCTGGCTCC 220 539 1157 Antisense
GCGTTCGCCCAACTTTTGAGATACG 268 291 1195 Antisense
AAACCCACTTTCAATCAGGATTTCT 681 125 1267 Antisense
Sequence Source Affymetrix Proprietary Database