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[185127_s_at]

Description C32B5.10 /REP_DB=WormBase Gene ID /WP=CE08518 /TR=P91104 /GB=AAB37966.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 26      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 14
(3)
A 		2.2
(-3)
A 		3.5
(-2.3)
A 		4.1
(-2)
A 		12
B 2.6
(-3)
A 		22.2
(3)
A 		16.4
(1.2)
A 		4.9
(-2.3)
A 		20
C 6
(-2.3)
A 		4.6
(-2.7)
A 		3.4
(-3)
A 		25.4
(3)
A 		22
avr 7.5
(-3) 		9.7
(0.3) 		7.8
(-2.6) 		11.5
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 6.3
(-2.7)
A 		6.7
(-2.7)
A 		2
(-3)
A 		4.4
(-2.9)
A 		66.3
(0.8)
A 		104.3
(3)
A 		102
B 5.9
(-2.7)
A 		2.3
(-3)
A 		3.2
(-2.9)
A 		2.9
(-3)
A 		51.1
(0.6)
A 		84.2
(3)
A 		82
C 31.2
(-1)
A 		3.9
(-2.9)
A 		24.8
(-1.4)
A 		3.1
(-3)
A 		25.8
(-1.3)
A 		85.5
(3)
P 		82
avr 14.5
(-2.2) 		4.3
(-2.9) 		10
(-2.6) 		3.5
(-3) 		47.7
(0) 		91.3
(3) 		88
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 5.1
(3)
A 		0.5
(-3)
A 		0.9
(-2.5)
A 		0.6
(-2.9)
A 		5
B 0.8
(-3)
A 		5.5
(3)
A 		3.8
(0.8)
A 		1
(-2.7)
A 		5
C 2
(-1.5)
A 		1.3
(-2.8)
A 		1.2
(-3)
A 		4.3
(3)
A 		3
avr 2.6
(3) 		2.4
(1) 		2
(-3) 		2
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 1.2
(-2.8)
A 		2.4
(-2.5)
A 		0.4
(-3)
A 		1.1
(-2.8)
A 		20
(1.7)
A 		25.4
(3)
A 		25
B 2.1
(-2.7)
A 		0.8
(-3)
A 		0.8
(-3)
A 		0.8
(-3)
A 		20.8
(0.9)
A 		31.4
(3)
A 		31
C 11.6
(-0.6)
A 		1.2
(-2.9)
A 		6.3
(-1.8)
A 		0.6
(-3)
A 		8.4
(-1.3)
A 		28.6
(3)
P 		28
avr 5
(-2.1) 		1.5
(-2.8) 		2.5
(-2.6) 		0.8
(-3) 		16.4
(0.4) 		28.5
(3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185127_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5756
Sequence Type Exemplar sequence
Representative Public ID C32B5.10 NCBI
Target Description C32B5.10 /REP_DB=WormBase Gene ID /WP=CE08518 /TR=P91104 /GB=AAB37966.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061459(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061459 NCBI Caenorhabditis elegans C32B5.10 (C32B5.10) mRNA, complete cds. 11/11 None
SNAP00000042168 ENSEMBL cdna:SNAP chromosome:CEL140:II:946566:948187:-1 11/11 None
GENEFINDER00000042193 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:946566:948187:-1 11/11 None
C32B5.10 ENSEMBL cdna:known chromosome:CEL140:II:946526:948187:-1 gene:C32B5.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:946564-948186(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32B5.10
Entrez Gene 173476 Entrez gene
SwissProt P91104 EMBL-EBI
WormBase CE08518 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_493860 C32B5.10 [Caenorhabditis elegans] gb|AAB37966.1| Hypothetical protein C32B5.10 [Caenorhabditis elegans] 0.0
blast AAB66238 Hypothetical protein R07C3.8 [Caenorhabditis elegans] ref|NP_493849.1| R07C3.8 [Caenorhabditis elegans] 1.0E-97
blast AAB66230 Hypothetical protein R07C3.9 [Caenorhabditis elegans] ref|NP_493848.1| R07C3.9 [Caenorhabditis elegans] 1.0E-57
Sequence
Target Sequence 
>C. ELEGANS:185127_S_AT
gatccttagagttccagcaagcctcaacttgaaggtgacccatttggaaatcctgggctc
cggtgtggataaaatcgcaatttcctttcaaccactgctagaaggatccagcctgcaatc
catcaaactgaacaatgacttcaacatcgaaaatcctatactccaaacctccccactcct
gatcttcaagaacccaaatgaaaacctgttccgagccagatcagcattcaccaatgttcg
agtagagctggaaaagtcgaccatcttcccacgagaagctttggaccttattgcgaattg
gttgagacatgggaaggaagtcgggacgatttggaatttttcactgaccgaggttacagc
gctagtgtgtttcaaggatatccggaagctgcctggcgcaaagtatgcaggacataaaac
aagctctcaactcggacgtagtgttctcatccctatcaacgcattttcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCCTTAGAGTTCCAGCAAGCCTC 641 415 693 Antisense
AGCCTCAACTTGAAGGTGACCCATT 422 81 712 Antisense
AATTTCCTTTCAACCACTGCTAGAA 701 173 771 Antisense
TCCAGCCTGCAATCCATCAAACTGA 486 587 799 Antisense
TCGAAAATCCTATACTCCAAACCTC 451 599 839 Antisense
TTCCGAGCCAGATCAGCATTCACCA 113 697 901 Antisense
TGGAAAAGTCGACCATCTTCCCACG 658 545 941 Antisense
GACCGAGGTTACAGCGCTAGTGTGT 549 381 1038 Antisense
GGAAGCTGCCTGGCGCAAAGTATGC 708 527 1076 Antisense
AACTCGGACGTAGTGTTCTCATCCC 580 139 1121 Antisense
CTCATCCCTATCAACGCATTTTCGG 536 229 1138 Antisense
Sequence Source Affymetrix Proprietary Database