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[185114_at]

Description Y66H1A.3 /REP_DB=WormBase Gene ID /WP=CE22754 /TR=Q9TYJ8 /GB=AAD12855.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 66      6grp_total > 34      4grp_total > 09      6grp_highest_sub > 01_02_03      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1910
1 2 3 4 range
A 1207.8
(-3)
P 		1535.6
(-2)
P 		1481.4
(-2.1)
P 		3118.2
(3)
P 		1910
B 1371.3
(-3)
P 		1494.2
(-2.3)
P 		1538.8
(-2.1)
P 		2464.9
(3)
P 		1094
C 1327.2
(-3)
P 		1502.3
(-1.8)
P 		1361.2
(-2.8)
P 		2188.9
(3)
P 		862
avr 1302.1
(-3) 		1510.7
(-2) 		1460.5
(-2.3) 		2590.7
(3) 		1289

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 761
1 2 3 4 5 6 range
A 1681
(2.4)
P 		1284.9
(-1.3)
P 		1746
(3)
P 		1262.1
(-1.6)
P 		1285.6
(-1.3)
P 		1109.5
(-3)
P 		637
B 1641.7
(0.3)
P 		1649
(0.4)
P 		1870.3
(3)
P 		1472.1
(-1.7)
P 		1518
(-1.1)
P 		1358.5
(-3)
P 		512
C 1357.4
(-0.5)
P 		1703.2
(3)
P 		1245.2
(-1.7)
P 		1273.5
(-1.4)
P 		1113.4
(-3)
P 		1382
(-0.3)
P 		590
avr 1560
(1.9) 		1545.7
(1.7) 		1620.5
(3) 		1335.9
(-2.1) 		1305.7
(-2.6) 		1283.3
(-3) 		337
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 192
1 2 3 4 range
A 439.8
(2.2)
P 		333.3
(-3)
P 		387.3
(-0.4)
P 		456.4
(3)
P 		123
B 437.3
(-0.1)
P 		369
(-2.6)
P 		356.6
(-3)
P 		525.3
(3)
P 		169
C 439.3
(1)
P 		431.9
(0.6)
P 		474.4
(3)
P 		369.1
(-3)
P 		105
avr 438.8
(2) 		378.1
(-3) 		406.1
(-0.7) 		450.3
(3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 361
1 2 3 4 5 6 range
A 309.9
(-1.7)
P 		457
(3)
P 		380.2
(0.5)
P 		320.1
(-1.4)
P 		387.9
(0.8)
P 		270.1
(-3)
P 		187
B 571.2
(1.8)
P 		548.9
(1.2)
P 		454.2
(-1.3)
P 		389.9
(-3)
P 		616.7
(3)
P 		506.6
(0.1)
P 		227
C 503.1
(2.6)
P 		519.7
(3)
P 		316.9
(-1.6)
P 		256.1
(-3)
P 		360.3
(-0.6)
P 		462.6
(1.7)
P 		264
avr 461.4
(1.5) 		508.5
(3) 		383.8
(-1) 		322
(-3) 		455
(1.3) 		413.1
(-0.1) 		187
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185114_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11104
Sequence Type Exemplar sequence
Representative Public ID Y66H1A.3 NCBI
Target Description Y66H1A.3 /REP_DB=WormBase Gene ID /WP=CE22754 /TR=Q9TYJ8 /GB=AAD12855.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067529(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067529 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-313) (tag-313) mRNA, complete cds. 11/11 None
GENEFINDER00000007354 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:443997:444696:1 11/11 None
Y66H1A.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:443985:444767:1 gene:Y66H1A.3 11/11 None
Y66H1A.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:443985:444758:1 gene:Y66H1A.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:443105-444696(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-313
Ensembl Y66H1A.3
Entrez Gene 176873 Entrez gene
SwissProt Q9TYJ8 EMBL-EBI
WormBase CE31659 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD12855 Temporarily assigned gene name protein 313 [Caenorhabditis elegans] ref|NP_499930.2| Temporarily Assigned Gene name family member (tag-313) [Caenorhabditis elegans] 7.0E-66
blast AAD12855 Temporarily assigned gene name protein 313 [Caenorhabditis elegans] ref|NP_499930.2| Temporarily Assigned Gene name family member (tag-313) [Caenorhabditis elegans] 2.0E-63
blast CAE67899 Hypothetical protein CBG13496 [Caenorhabditis briggsae] 3.0E-52
blast XP_693156 PREDICTED: similar to mitochondrial ribosomal protein L55 isoform a [Danio rerio] 1.0E-11
Sequence
Target Sequence 
>C. ELEGANS:185114_AT
atgaacactcaaatttccatgcgaaacttgctaacaagctgctcaaaagccatcacagca
ggttagtaaaggaaaaaggtcgaaaaatatcatttgaagagagaaataatgcgtggagag
caagtttgggaaaaatcagccgtcgagactacttgcatcggtaccaagttaaatttatcc
gtccggatggttcgacaatcatggttcccgccgctgaaccgagacagacattccaatctg
ccgtcgacctcaaacagttgagcgaggatgaacgacgacaacggctggctgctcgtaaac
caaaagccaagatcacaaaaacagaagttatcgatgacaccttcgatgagtcggagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAACACTCAAATTTCCATGCGAA 83 45 62 Antisense
ACAAGCTGCTCAAAAGCCATCACAG 224 113 95 Antisense
GGGAAAAATCAGCCGTCGAGACTAC 136 493 189 Antisense
GTCGAGACTACTTGCATCGGTACCA 552 473 203 Antisense
TATCCGTCCGGATGGTTCGACAATC 244 625 237 Antisense
ATGGTTCGACAATCATGGTTCCCGC 194 51 248 Antisense
CGCCGCTGAACCGAGACAGACATTC 431 257 270 Antisense
AGACAGACATTCCAATCTGCCGTCG 352 71 283 Antisense
CTGCCGTCGACCTCAAACAGTTGAG 545 233 299 Antisense
ATGAACGACGACAACGGCTGGCTGC 36 45 329 Antisense
GATGACACCTTCGATGAGTCGGAGT 124 413 394 Antisense
Sequence Source Affymetrix Proprietary Database