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[185109_at]

Description T13A10.5 /REP_DB=WormBase Gene ID /WP=CE07486 /TR=Q22443 /GB=AAB38118.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 03      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 60      6grp_total > 25      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 383
1 2 3 4 range
A 468.2
(3)
P 		250.5
(-1.9)
P 		200.9
(-3)
P 		346.6
(0.3)
P 		267
B 483
(3)
P 		298.5
(-1.6)
P 		244.4
(-3)
P 		274.8
(-2.2)
P 		239
C 584.1
(3)
P 		353.7
(-1.3)
P 		262.5
(-3)
P 		357.2
(-1.2)
P 		322
avr 511.8
(3) 		300.9
(-1.6) 		235.9
(-3) 		326.2
(-1) 		276

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 634
1 2 3 4 5 6 range
A 495.7
(0.3)
P 		741.3
(3)
P 		452.9
(-0.2)
P 		409.9
(-0.7)
P 		197.4
(-3)
P 		293
(-1.9)
P 		544
B 629.3
(3)
P 		554.1
(1.9)
P 		468.7
(0.7)
P 		255.3
(-2.3)
P 		203.7
(-3)
P 		229.2
(-2.6)
P 		426
C 831.8
(3)
P 		467.8
(-0.6)
P 		469.2
(-0.6)
P 		278.7
(-2.4)
P 		219.9
(-3)
P 		360.5
(-1.6)
P 		612
avr 652.3
(3) 		587.7
(2.1) 		463.6
(0.5) 		314.6
(-1.6) 		207
(-3) 		294.2
(-1.8) 		445
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 170.5
(3)
P 		54.4
(-2.8)
P 		52.5
(-2.9)
P 		50.7
(-3)
P 		120
B 154
(3)
P 		73.7
(-1.9)
P 		56.6
(-3)
P 		58.6
(-2.9)
P 		97
C 193.3
(3)
P 		101.7
(-1.1)
P 		91.5
(-1.6)
P 		60.2
(-3)
P 		133
avr 172.6
(3) 		76.6
(-2) 		66.9
(-2.5) 		56.5
(-3) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 252
1 2 3 4 5 6 range
A 91.4
(-2.1)
P 		263.6
(3)
P 		98.6
(-1.9)
P 		104
(-1.7)
P 		59.6
(-3)
P 		71.3
(-2.7)
P 		204
B 219
(3)
P 		184.4
(1.6)
P 		113.8
(-1.2)
P 		67.6
(-3)
P 		82.7
(-2.4)
P 		85.5
(-2.3)
P 		151
C 308.3
(3)
P 		142.7
(-0.9)
P 		119.4
(-1.5)
P 		56
(-3)
P 		71.1
(-2.6)
P 		120.7
(-1.5)
P 		252
avr 206.2
(3) 		196.9
(2.6) 		110.6
(-1.2) 		75.9
(-2.8) 		71.1
(-3) 		92.5
(-2) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185109_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10660
Sequence Type Exemplar sequence
Representative Public ID T13A10.5 NCBI
Target Description T13A10.5 /REP_DB=WormBase Gene ID /WP=CE07486 /TR=Q22443 /GB=AAB38118.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028213(10), NM_001028214(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028213 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-16) (nlp-16) mRNA, complete cds. 10/11 None
NM_001028214 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-16) (nlp-16) mRNA, complete cds. 10/11 None
SNAP00000039089 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6258812:6259802:-1 11/11 None
GENEFINDER00000039100 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6255317:6259802:-1 10/11 None
T13A10.5a ENSEMBL cdna:known chromosome:CEL140:IV:6255096:6259802:-1 gene:T13A10.5 10/11 A 
T13A10.5b ENSEMBL cdna:known chromosome:CEL140:IV:6255342:6259805:-1 gene:T13A10.5 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6258817-6259808(-) 100.0 100.0
Public Domain and Genome References
Ensembl T13A10.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023384 Neuropeptide-Like Protein family member (nlp-16) [Caenorhabditis elegans] gb|AAB38119.2| Neuropeptide-like protein protein 16, isoform a [Caenorhabditis elegans] 1.0E-168
blast T16849 hypothetical protein T13A10.5 - Caenorhabditis elegans 1.0E-126
blast CAE61576 Hypothetical protein CBG05490 [Caenorhabditis briggsae] 1.0E-123
blast NP_001023384 Neuropeptide-Like Protein family member (nlp-16) [Caenorhabditis elegans] gb|AAB38119.2| Neuropeptide-like protein protein 16, isoform a [Caenorhabditis elegans] 1.0E-114
blast T16849 hypothetical protein T13A10.5 - Caenorhabditis elegans 1.0E-109
Sequence
Target Sequence 
>C. ELEGANS:185109_AT
gaagtttccgaggatccaacacgagagaaacgtggtttgtacagttctgaaagaactgaa
gaagaagttgaaatcagtcatggaatgcatcatcgggagaagcgacactctgagcatcta
cctcatccagatccaccaagtcacacagctaagagaagtacagaacatcatagagttaaa
agatcagaaggtcatccacatgaaaagaaggctacccattctccagaaggacacattgtc
gcaaaagatgatcatcacgggcatgaaaagagaagttctgatagccatcatggacatcag
aagcgtagtgtagatgaacatcatggacaccaaaagaagaatgctgaagatcatcacgag
catcagaagagatcggagcatgttgagcatcaggcggaaatgcatgaacatcagaagaga
agtacacaagaagtttctggacatccagagcatcatttggttaagagaagtggaggagaa
ggaggtgagccagattgtatcactagtcgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTTTCCGAGGATCCAACACGAG 519 335 187 Antisense
GAAACGTGGTTTGTACAGTTCTGAA 331 357 213 Antisense
GAGAAGCGACACTCTGAGCATCTAC 423 391 283 Antisense
TCTGAGCATCTACCTCATCCAGATC 344 609 295 Antisense
ATCCACCAAGTCACACAGCTAAGAG 204 39 317 Antisense
GAAAAGAAGGCTACCCATTCTCCAG 79 355 388 Antisense
GCTACCCATTCTCCAGAAGGACACA 433 303 397 Antisense
GAAGTTCTGATAGCCATCATGGACA 372 337 458 Antisense
CGGAGCATGTTGAGCATCAGGCGGA 679 241 560 Antisense
GGACATCCAGAGCATCATTTGGTTA 297 525 625 Antisense
GAGCCAGATTGTATCACTAGTCGTT 411 383 673 Antisense
Sequence Source Affymetrix Proprietary Database