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[185086_at]

Description F47C12.4 /REP_DB=WormBase Gene ID /WP=CE10676 /TR=Q20528 /GB=AAC24390.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 77      4grp_total > 10      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 1.7
(-3)
A 		2.1
(-2.1)
A 		2
(-2.3)
A 		4.4
(3)
A 		3
B 2.7
(3)
A 		2
(-3)
A 		2
(-3)
A 		2.7
(3)
A 		1
C 3.6
(-3)
A 		4.8
(-0.9)
A 		3.9
(-2.5)
A 		7.1
(3)
A 		4
avr 2.7
(-2.7) 		3
(-1.9) 		2.6
(-3) 		4.7
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 3.6
(-2.9)
A 		3.6
(-2.9)
A 		2.2
(-3)
A 		3.3
(-2.9)
A 		4.7
(-2.9)
A 		134
(3)
P 		132
B 1.3
(-3)
A 		3.7
(-2.9)
A 		4.1
(-2.8)
A 		2.6
(-2.9)
A 		61.6
(0.4)
A 		108.8
(3)
P 		108
C 1.9
(-3)
A 		2.1
(-3)
A 		3.1
(-2.9)
A 		4.4
(-2.9)
A 		4.9
(-2.9)
A 		124.6
(3)
P 		123
avr 2.3
(-3) 		3.1
(-3) 		3.1
(-3) 		3.4
(-2.9) 		23.7
(-1.9) 		122.5
(3) 		120
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 0.6
(3)
A 		0.5
(-3)
A 		0.5
(-3)
A 		0.6
(3)
A 		0
B 0.8
(3)
A 		0.5
(-3)
A 		0.5
(-3)
A 		0.6
(-1)
A 		0
C 1.2
(-3)
A 		1.4
(3)
A 		1.4
(3)
A 		1.2
(-3)
A 		0
avr 0.9
(3) 		0.8
(-3) 		0.8
(-3) 		0.8
(-3) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 0.7
(-3)
A 		1.3
(-2.9)
A 		0.5
(-3)
A 		0.8
(-2.9)
A 		1.4
(-2.8)
A 		32.6
(3)
P 		32
B 0.4
(-3)
A 		1.2
(-2.9)
A 		1
(-2.9)
A 		0.7
(-3)
A 		25
(0.7)
A 		40.6
(3)
P 		40
C 0.7
(-3)
A 		0.7
(-3)
A 		0.8
(-3)
A 		0.9
(-3)
A 		1.6
(-2.9)
A 		41.7
(3)
P 		41
avr 0.6
(-3) 		1.1
(-2.9) 		0.8
(-3) 		0.8
(-3) 		9.3
(-1.6) 		38.3
(3) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185086_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11231
Sequence Type Exemplar sequence
Representative Public ID F47C12.4 NCBI
Target Description F47C12.4 /REP_DB=WormBase Gene ID /WP=CE10676 /TR=Q20528 /GB=AAC24390.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068049(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068049 NCBI Caenorhabditis elegans F47C12.4 (F47C12.4) mRNA, complete cds. 11/11 A 
SNAP00000026878 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3973362:3975657:1 11/11 None
GENEFINDER00000026891 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3973362:3983603:1 11/11 None
F47C12.4 ENSEMBL cdna:known chromosome:CEL140:IV:3973343:3975707:1 gene:F47C12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3973367-3975663(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F47C12.4
Entrez Gene 177154 Entrez gene
SwissProt Q20528 EMBL-EBI
WormBase CE10676 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5529 sugar binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC24390 Hypothetical protein F47C12.4 [Caenorhabditis elegans] ref|NP_500450.1| F47C12.4 [Caenorhabditis elegans] 0.0
blast AAF60596 Hypothetical protein Y46C8AL.1 [Caenorhabditis elegans] ref|NP_500445.1| Y46C8AL.1 [Caenorhabditis elegans] 0.0
blast AAC24394 Serpentine receptor, class h protein 223 [Caenorhabditis elegans] ref|NP_500453.1| Serpentine Receptor, class H family member (srh-223) [Caenorhabditis elegans] 0.0
blast CAE71799 Hypothetical protein CBG18810 [Caenorhabditis briggsae] 1.0E-108
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500450.1 1 5-27
F47C12.4 1 5-27
SNAP00000026878 1 5-27
GENEFINDER00000026891 8 5-27,601-623,644-666,681-700,721-740,778-800,821-843,853-875
Sequence
Target Sequence 
>C. ELEGANS:185086_AT
gaagtacaagtttgatgttccaccgggatggcttgacttccgtcgactcaatggacaagt
ggtcctgcattggctctcggaagaaaaactaaagtttacagacgcagccaaagcatgtga
ggcgaaaggagcacacttggctggtattaattcgctgaaagaagcagaacagttaggaaa
tcatgttgtcggagctaagttgaacaacgagcagttctggcttggagcgcaacgtaagac
cgaatgcttggaaaataacgactggaaagctccttgcacacgtggcaaaatcttcgaatg
gaacaataacgtggcgacggatttccgtgaagagtggtggaagaaaaataacaataacca
ttctccaaatccatcaggcactggtcaacgatgcttgtcttttgcctttggggatctgta
ttggtcagagaaaggagataaaggatttttggatgacattgagtgccacaacgagctgag
atacttctgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTACAAGTTTGATGTTCCACCG 280 337 1206 Antisense
GTTTGATGTTCCACCGGGATGGCTT 286 447 1215 Antisense
ATGTTCCACCGGGATGGCTTGACTT 8 47 1220 Antisense
CTGCATTGGCTCTCGGAAGAAAAAC 540 235 1270 Antisense
AACTAAAGTTTACAGACGCAGCCAA 466 107 1292 Antisense
GACGCAGCCAAAGCATGTGAGGCGA 211 381 1306 Antisense
GCATGTGAGGCGAAAGGAGCACACT 96 311 1318 Antisense
AGGAGCACACTTGGCTGGTATTAAT 57 55 1332 Antisense
GCACACTTGGCTGGTATTAATTCGC 567 317 1336 Antisense
GTGCCACAACGAGCTGAGATACTTC 151 479 1668 Antisense
CACAACGAGCTGAGATACTTCTGCA 95 195 1672 Antisense
Sequence Source Affymetrix Proprietary Database