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[185074_at]

Description F28A12.2 /REP_DB=WormBase Gene ID /WP=CE09736 /TR=Q22969 /GB=AAC47990.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 33      4grp_high_8_8 > 46      6grp_highest_nonorm > 90      6grp_total > 21      4grp_total > 41      6grp_highest_sub > 02_03      6grp_cgc > 62      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 79.1
(-3)
P 		150.5
(0.3)
P 		207.9
(3)
P 		98.7
(-2.1)
P 		129
B 63
(-3)
P 		233
(3)
P 		188.3
(1.4)
P 		69
(-2.8)
P 		170
C 52
(-3)
A 		202.4
(3)
P 		165.9
(1.5)
P 		182
(2.2)
P 		150
avr 64.7
(-3) 		195.3
(3) 		187.4
(2.6) 		116.6
(-0.6) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 139.5
(2)
P 		151.4
(3)
P 		127.6
(0.9)
P 		108.2
(-0.8)
P 		95.4
(-1.9)
P 		82.5
(-3)
P 		69
B 75.6
(-2.9)
P 		102.2
(0.2)
P 		126.4
(3)
P 		104.9
(0.5)
P 		102.4
(0.2)
P 		74.3
(-3)
P 		52
C 94.7
(-1.6)
P 		135.4
(3)
P 		95.3
(-1.6)
P 		121.8
(1.4)
P 		106.9
(-0.3)
P 		82.8
(-3)
P 		53
avr 103.3
(-0.2) 		129.7
(3) 		116.4
(1.4) 		111.6
(0.8) 		101.6
(-0.4) 		79.9
(-3) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 28.8
(-0.8)
P 		32.7
(-0.3)
P 		54.4
(3)
P 		14.4
(-3)
P 		40
B 20.1
(-2.2)
P 		57.6
(3)
P 		43.6
(1)
P 		14.7
(-3)
P 		43
C 17.2
(-3)
A 		58.2
(3)
P 		57.8
(2.9)
P 		30.7
(-1)
P 		41
avr 22
(-2.6) 		49.5
(2.6) 		51.9
(3) 		19.9
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 25.7
(-2)
P 		53.8
(3)
P 		27.8
(-1.6)
P 		27.5
(-1.7)
P 		28.8
(-1.5)
P 		20.1
(-3)
P 		34
B 26.3
(-3)
P 		34
(0)
P 		30.7
(-1.3)
P 		27.8
(-2.4)
P 		41.6
(3)
P 		27.7
(-2.5)
P 		15
C 35.1
(0.8)
P 		41.3
(3)
P 		24.3
(-3)
P 		24.5
(-2.9)
P 		34.6
(0.6)
P 		27.7
(-1.8)
P 		17
avr 29
(-1.7) 		43
(3) 		27.6
(-2.2) 		26.6
(-2.5) 		35
(0.3) 		25.2
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185074_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11359
Sequence Type Exemplar sequence
Representative Public ID F28A12.2 NCBI
Target Description F28A12.2 /REP_DB=WormBase Gene ID /WP=CE09736 /TR=Q22969 /GB=AAC47990.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072828(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072828 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-12) (grl-12) mRNA, complete cds. 9/11 None
GENEFINDER00000012221 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8638526:8640206:-1 11/11 None
F28A12.2 ENSEMBL cdna:known chromosome:CEL140:V:8638503:8639576:-1 gene:F28A12.2 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000012213 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8638522-8640207(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-12
Ensembl F28A12.2
Entrez Gene 185037 Entrez gene
SwissProt Q22969 EMBL-EBI
WormBase CE34830 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE58345 Hypothetical protein CBG01466 [Caenorhabditis briggsae] 3.0E-82
blast AAC47990 Ground-like (grd related) protein 12 [Caenorhabditis elegans] ref|NP_505229.2| GRound-Like (grd related) family member (grl-12) [Caenorhabditis elegans] 4.0E-81
blast AAC47990 Ground-like (grd related) protein 12 [Caenorhabditis elegans] ref|NP_505229.2| GRound-Like (grd related) family member (grl-12) [Caenorhabditis elegans] 4.0E-80
blast CAE58345 Hypothetical protein CBG01466 [Caenorhabditis briggsae] 1.0E-61
blast CAE64618 Hypothetical protein CBG09376 [Caenorhabditis briggsae] 7.0E-17
blast CAE64618 Hypothetical protein CBG09376 [Caenorhabditis briggsae] 2.0E-16
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 5.8E-32
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 5.8E-32
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000012221 1 131-153
Sequence
Target Sequence 
>C. ELEGANS:185074_AT
cacagtttcattccacagtcatcttcttttctttcaccagaatcatccaactttccattg
cgatgctttcaccgttccttcttctttttcttctcttttctgctccagcttccgcattct
ttaattcattattcggtggatcaaattgtggatgctcgtgtactcctccaccgtctacat
gtccaactacttgtcttccaatcatgacgtgtactccaccttccccgccggcatgttgta
acacatgtggaacttgtaacggaaagagaagaagacggcaccttacaatgttaagtaatg
ctacatatgtggcagatcatgaaaaagtgaaccgagtaaagcgacaagacgatcaagaga
caaccgtaacatcaggaaactgcaactctgtcgaactacaaagaattatcgaaagtaaaa
tagaccgtgtgactgcgattgcaaagcgaagaattcaagaagaagccgaggcaacgatgg
gtggccgattcaatgttatctgtgcccgaggcgacttttcatacgtggcaaatactgaac
ttttctgtcaacattctgtcggtgatgtcacgtgtttcttgttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACAGTTTCATTCCACAGTCATCTT 556 195 362 Antisense
AACTTTCCATTGCGATGCTTTCACC 312 139 410 Antisense
CAGCTTCCGCATTCTTTAATTCATT 185 205 467 Antisense
AATTGTGGATGCTCGTGTACTCCTC 450 179 505 Antisense
TCCAACTACTTGTCTTCCAATCATG 196 587 543 Antisense
CAATCATGACGTGTACTCCACCTTC 467 179 560 Antisense
GGAAACTGCAACTCTGTCGAACTAC 179 527 736 Antisense
ATAGACCGTGTGACTGCGATTGCAA 220 23 781 Antisense
ATTCAATGTTATCTGTGCCCGAGGC 709 11 849 Antisense
GAACTTTTCTGTCAACATTCTGTCG 489 349 898 Antisense
GGTGATGTCACGTGTTTCTTGTTCA 363 501 922 Antisense
Sequence Source Affymetrix Proprietary Database