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[185056_at]

Description F37C12.2 /REP_DB=WormBase Gene ID /WP=CE00741 /TR=Q20123 /GB=AAC48294.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 98      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 95      6grp_total > 95      4grp_total > 05      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 562
1 2 3 4 range
A 716.7
(0.9)
P 		368.5
(-3)
P 		432.5
(-2.3)
P 		903
(3)
P 		535
B 581.3
(-0.3)
P 		352.4
(-2.9)
P 		341.1
(-3)
P 		874.5
(3)
P 		533
C 535.7
(-1)
P 		428.2
(-2.5)
P 		388.8
(-3)
P 		825.8
(3)
P 		437
avr 611.2
(-0.2) 		383
(-3) 		387.5
(-2.9) 		867.8
(3) 		485

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 620
1 2 3 4 5 6 range
A 558.9
(-2.2)
P 		485.6
(-3)
P 		508.5
(-2.7)
P 		917.4
(1.9)
P 		947.4
(2.3)
P 		1009.9
(3)
P 		524
B 588.6
(-0.9)
P 		550.8
(-1.3)
P 		390.4
(-3)
P 		735.7
(0.6)
P 		943.4
(2.8)
P 		960.3
(3)
P 		570
C 687.8
(0.1)
P 		446.5
(-3)
P 		831.3
(1.9)
P 		675.9
(-0.1)
P 		920.5
(3)
P 		847.5
(2.1)
P 		474
avr 611.8
(-1.4) 		494.3
(-3) 		576.7
(-1.9) 		776.3
(0.8) 		937.1
(3) 		939.2
(3) 		445
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 261
(3)
P 		80
(-3)
P 		113.1
(-1.9)
P 		132.2
(-1.3)
P 		181
B 185.4
(2.9)
P 		87
(-2.6)
P 		79
(-3)
P 		186.4
(3)
P 		107
C 177.3
(3)
P 		123.1
(-3)
P 		135.5
(-1.6)
P 		139.2
(-1.2)
P 		54
avr 207.9
(3) 		96.7
(-3) 		109.2
(-2.3) 		152.6
(0) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 289
1 2 3 4 5 6 range
A 103
(-3)
P 		172.7
(-0.7)
P 		110.7
(-2.7)
P 		232.7
(1.3)
P 		285.9
(3)
P 		245.9
(1.7)
P 		183
B 204.8
(-0.7)
P 		183.3
(-1.2)
P 		94.8
(-3)
P 		194.9
(-0.9)
P 		383.3
(3)
P 		358.1
(2.5)
P 		289
C 254.9
(1.4)
P 		136.2
(-3)
P 		211.6
(-0.2)
P 		135.9
(-3)
P 		297.9
(3)
P 		283.7
(2.5)
P 		162
avr 187.6
(-1.4) 		164.1
(-2.2) 		139
(-3) 		187.8
(-1.4) 		322.4
(3) 		295.9
(2.1) 		183
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185056_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7293
Sequence Type Exemplar sequence
Representative Public ID F37C12.2 NCBI
Target Description F37C12.2 /REP_DB=WormBase Gene ID /WP=CE00741 /TR=Q20123 /GB=AAC48294.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066174(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066174 NCBI Caenorhabditis elegans F37C12.2 (F37C12.2) mRNA, complete cds. 11/11 None
SNAP00000030591 ENSEMBL cdna:SNAP chromosome:CEL140:III:7182230:7183740:1 11/11 None
GENEFINDER00000030608 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7182230:7183740:1 11/11 None
F37C12.2 ENSEMBL cdna:known chromosome:CEL140:III:7181915:7183910:1 gene:F37C12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7182227-7183738(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F37C12.2
Entrez Gene 176009 Entrez gene
SwissProt Q20123 EMBL-EBI
WormBase CE00741 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.17014.1.S1_AT similar to etoposide induced 2.4 isoform 1 cfa
DROSOPHILA_2:1636654_S_AT dm
DROSGENOME1:145782_AT dm
CHICKEN:GGA.8073.1.S1_S_AT etoposide induced 2.4 mRNA gga
HG-U133_PLUS_2:216396_S_AT etoposide induced 2.4 mRNA hs
HG-U133A_2:216396_S_AT etoposide induced 2.4 mRNA hs
HG-U133A:216396_S_AT etoposide induced 2.4 mRNA hs
HG-U133_PLUS_2:208289_S_AT etoposide induced 2.4 mRNA hs
HG-U133A:208289_S_AT etoposide induced 2.4 mRNA hs
HG-FOCUS:208289_S_AT etoposide induced 2.4 mRNA hs
HG-U133A_2:208289_S_AT etoposide induced 2.4 mRNA hs
HU35KSUBA:RC_AA457242_AT etoposide induced 2.4 mRNA hs
HU35KSUBD:RC_N79610_AT etoposide induced 2.4 mRNA hs
U133_X3P:G6631103_3P_A_AT etoposide induced 2.4 mRNA hs
U133_X3P:HS.286027.3.S1_3P_A_AT etoposide induced 2.4 mRNA hs
HG-U95AV2:38097_AT etoposide induced 2.4 mRNA hs
HG-U95C:64331_F_AT etoposide induced 2.4 mRNA hs
HG-U95C:65566_AT etoposide induced 2.4 mRNA hs
MU11KSUBA:U41751_S_AT etoposide induced 2.4 mRNA mm
MG-U74AV2:99629_AT etoposide induced 2.4 mRNA mm
MG-U74CV2:134791_AT etoposide induced 2.4 mRNA mm
MOE430A:1416555_AT etoposide induced 2.4 mRNA mm
MOUSE430A_2:1416555_AT etoposide induced 2.4 mRNA mm
MOUSE430_2:1416555_AT etoposide induced 2.4 mRNA mm
MU19KSUBC:TC41663_S_AT Etoposide induced 2.4 mRNA (Ei24), mRNA mm
RG-U34B:RC_AA850247_AT etoposide induced 2.4 mRNA (predicted) rn
RAE230A:1388642_AT etoposide induced 2.4 mRNA (predicted) rn
RAT230_2:1388642_AT etoposide induced 2.4 mRNA (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAC48294 Hypothetical protein F37C12.2 [Caenorhabditis elegans] ref|NP_498575.1| F37C12.2 [Caenorhabditis elegans] 1.0E-162
blast CAE63802 Hypothetical protein CBG08348 [Caenorhabditis briggsae] 1.0E-79
blast XP_395483 PREDICTED: similar to ENSANGP00000010382 [Apis mellifera] 4.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR009890 EMBL-EBI Etoposide-induced 2.4 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498575.1 5 79-101,114-136,151-173,180-202,247-269
F37C12.2 5 79-101,114-136,151-173,180-202,247-269
SNAP00000030591 5 79-101,114-136,151-173,180-202,247-269
GENEFINDER00000030608 5 79-101,114-136,151-173,180-202,247-269
Sequence
Target Sequence 
>C. ELEGANS:185056_AT
tcggctacattctgattattcccatcttttttgcttctcgaatcattcaggcactttggt
tctcagatatttcaggagcctgtatgagagctctaaaattgccaccaccacctgtcgttc
cttttagttctatgttagcgggaactctcatttctgcgctccatcaaatttttttcctca
ttcaaggaatgctttcgcaatatcttccaattcctcttatcacaccagttatcgtttatc
ttcatatggctctacttaactcaatgtactgtttcgattacttcttcgacggttacaacc
tttcattccttcgacgcaaagatattttcgaatcacactggccgtacttccttggttttg
gaactcccctggcacttgcttgctcaatcagcagtaatatgtttgtgaacagtgtcattt
ttgcacttctcttcccatttttcatcatcaccagctatccggcaaattggaatagaaaat
acgaagaggaaattccgaaaattgcattctgccgtatttcataca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGGCTACATTCTGATTATTCCCAT 462 607 350 Antisense
TCATTCAGGCACTTTGGTTCTCAGA 305 619 392 Antisense
GAGAGCTCTAAAATTGCCACCACCA 183 395 435 Antisense
TAGCGGGAACTCTCATTTCTGCGCT 513 647 485 Antisense
TGCGCTCCATCAAATTTTTTTCCTC 573 583 504 Antisense
TGCTTTCGCAATATCTTCCAATTCC 414 579 539 Antisense
TTCCTCTTATCACACCAGTTATCGT 649 697 560 Antisense
ATTTTCGAATCACACTGGCCGTACT 520 13 673 Antisense
GGCACTTGCTTGCTCAATCAGCAGT 564 535 720 Antisense
TCATCACCAGCTATCCGGCAAATTG 156 621 794 Antisense
ATTGCATTCTGCCGTATTTCATACA 158 7 850 Antisense
Sequence Source Affymetrix Proprietary Database