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[185041_at]

Description T25G12.8 /REP_DB=WormBase Gene ID /WP=CE07545 /TR=Q22786 /GB=AAC69021.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 25      4grp_high_8_8 > 35      6grp_highest_nonorm > 90      6grp_total > 25      4grp_total > 53      6grp_highest_sub > De      6grp_high1_sub > 01_02      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 152.4
(3)
P 		114.7
(1)
P 		85.8
(-0.5)
P 		39.1
(-3)
A 		113
B 137.3
(1.4)
P 		162
(3)
P 		90.1
(-1.5)
P 		66.5
(-3)
P 		96
C 148
(3)
P 		129.7
(2.1)
P 		79.4
(-0.6)
P 		32.2
(-3)
A 		116
avr 145.9
(3) 		135.5
(2.4) 		85.1
(-0.6) 		45.9
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 188.8
(3)
P 		142.6
(0.7)
P 		76.7
(-2.6)
P 		87.6
(-2.1)
P 		69.6
(-3)
P 		101
(-1.4)
P 		119
B 131.2
(2.2)
P 		142.2
(3)
P 		100.4
(-0.2)
P 		74.4
(-2.1)
P 		62.8
(-3)
P 		71.7
(-2.3)
P 		79
C 130.2
(0.9)
P 		144.2
(3)
P 		138.1
(2.1)
P 		120.3
(-0.7)
P 		123.8
(-0.1)
P 		105
(-3)
P 		39
avr 150.1
(3) 		143
(2.3) 		105.1
(-1.2) 		94.1
(-2.2) 		85.4
(-3) 		92.6
(-2.3) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 55.5
(3)
P 		24.9
(-0.7)
P 		22.4
(-1)
P 		5.7
(-3)
A 		50
B 43.8
(3)
P 		40
(2.2)
P 		20.9
(-1.6)
P 		14.2
(-3)
P 		30
C 49
(3)
P 		37.3
(1.4)
P 		27.7
(0.1)
P 		5.4
(-3)
A 		44
avr 49.4
(3) 		34.1
(0.8) 		23.7
(-0.8) 		8.4
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 34.8
(0.2)
P 		50.7
(3)
P 		16.7
(-3)
P 		22.2
(-2)
P 		21
(-2.2)
P 		24.6
(-1.6)
P 		34
B 45.7
(2.7)
P 		47.3
(3)
P 		24.4
(-2)
P 		19.7
(-3)
P 		25.5
(-1.7)
P 		26.7
(-1.5)
P 		28
C 48.2
(3)
P 		44
(2)
P 		35.2
(-0.3)
P 		24.2
(-3)
P 		40.1
(1)
P 		35.2
(-0.3)
P 		24
avr 42.9
(2) 		47.3
(3) 		25.4
(-2.2) 		22
(-3) 		28.9
(-1.4) 		28.8
(-1.4) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185041_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18039
Sequence Type Exemplar sequence
Representative Public ID T25G12.8 NCBI
Target Description T25G12.8 /REP_DB=WormBase Gene ID /WP=CE07545 /TR=Q22786 /GB=AAC69021.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078393(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078393 NCBI Caenorhabditis elegans T25G12.8 (T25G12.8) mRNA, complete cds. 11/11 None
SNAP00000027875 ENSEMBL cdna:SNAP chromosome:CEL140:X:17238002:17239352:-1 11/11 None
GENEFINDER00000027883 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17238002:17240278:-1 11/11 None
T25G12.8 ENSEMBL cdna:known chromosome:CEL140:X:17238002:17238772:-1 gene:T25G12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17238001-17238772(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25G12.8
Entrez Gene 188909 Entrez gene
SwissProt Q22786 EMBL-EBI
WormBase CE07545 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69021 Hypothetical protein T25G12.8 [Caenorhabditis elegans] ref|NP_510794.1| T25G12.8 [Caenorhabditis elegans] 1.0E-143
blast AAC69021 Hypothetical protein T25G12.8 [Caenorhabditis elegans] ref|NP_510794.1| T25G12.8 [Caenorhabditis elegans] 1.0E-140
blast CAA91053 Hypothetical protein F52E10.2 [Caenorhabditis elegans] ref|NP_510651.1| F52E10.2 [Caenorhabditis elegans] 3.0E-9
Sequence
Target Sequence 
>C. ELEGANS:185041_AT
attgatcaagacctctgcaaactgatggagggaatccaaatagacggagttcaaattgaa
ttaaagaagctagaagaatcagcatgtcgacaagaagacatgctccaaaaaatcaatgtg
aacatagagagatcccaaaagatgggatctcaaaatgaaatggatgcccaaaagatgggc
actcaaaagatgagtgacgagaatagcaaaagaaaattcggtgtccaaaggatggacacc
ggattgtcaaaagtcgaagccttgaagcaacatatgaagaactacttcgaacgaacaaat
caggatcatcaagaaggatgctcgagcaacttctccagcaagagatcatatgaggacaaa
aatgatgtacaaaggacttggaagcgtccgagaagggattatgcaacaagagactgcgga
agataccaatgttcattctgccctcgcacacacccttcgaatatgtgcggacaaatcaag
gctacagatgaaaggaaagcatttgtcaacttccgccaattgtgcaaaagctgtttgcaa
aaaccatgccgccaaagactgcatgcatcatgctactactgtcaatcaatggaccatcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGATCAAGACCTCTGCAAACTGA 364 3 109 Antisense
GACTTGGAAGCGTCCGAGAAGGGAT 213 369 483 Antisense
GAGACTGCGGAAGATACCAATGTTC 312 393 518 Antisense
CTCGCACACACCCTTCGAATATGTG 431 233 551 Antisense
ATGTGCGGACAAATCAAGGCTACAG 390 47 571 Antisense
GGAAAGCATTTGTCAACTTCCGCCA 510 527 602 Antisense
AACTTCCGCCAATTGTGCAAAAGCT 402 139 616 Antisense
GCTGTTTGCAAAAACCATGCCGCCA 407 295 638 Antisense
TGCCGCCAAAGACTGCATGCATCAT 527 583 655 Antisense
CATGCATCATGCTACTACTGTCAAT 33 207 670 Antisense
CTACTGTCAATCAATGGACCATCAT 116 227 684 Antisense
Sequence Source Affymetrix Proprietary Database