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[185040_at]

Description R12E2.12 /REP_DB=WormBase Gene ID /WP=CE18144 /TR=O61791 /GB=AAC17033.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 74      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 65      6grp_total > 74      4grp_total > 49      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 503
1 2 3 4 range
A 1203.7
(-3)
P 		1554.8
(3)
P 		1488.4
(1.9)
P 		1331.8
(-0.8)
P 		351
B 1144.6
(-1.9)
P 		1551.7
(3)
P 		1476.1
(2.1)
P 		1051.5
(-3)
P 		500
C 1177.8
(-3)
P 		1399.9
(0.7)
P 		1535.5
(3)
P 		1196.4
(-2.7)
P 		358
avr 1175.4
(-3) 		1502.1
(3) 		1500
(3) 		1193.2
(-2.7) 		327

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 641
1 2 3 4 5 6 range
A 994.2
(-2.7)
P 		981.1
(-3)
P 		1284.2
(3)
P 		1170.9
(0.8)
P 		1045.1
(-1.7)
P 		1133.3
(0)
P 		303
B 1023
(-3)
P 		1181.1
(-0.7)
P 		1320.5
(1.3)
P 		1349.4
(1.7)
P 		1290.1
(0.8)
P 		1439.9
(3)
P 		417
C 799.2
(-3)
P 		1202
(0.9)
P 		1181.1
(0.7)
P 		1303.9
(1.9)
P 		957.4
(-1.5)
P 		1414.2
(3)
P 		615
avr 938.8
(-3) 		1121.4
(-0.2) 		1261.9
(2) 		1274.7
(2.2) 		1097.5
(-0.6) 		1329.1
(3) 		390
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 340
1 2 3 4 range
A 438.3
(3)
P 		337.5
(0.5)
P 		389.1
(1.8)
P 		194.9
(-3)
P 		243
B 365
(2.3)
P 		383.2
(3)
P 		342
(1.4)
P 		224.1
(-3)
P 		159
C 389.9
(0.4)
P 		402.5
(0.6)
P 		535.2
(3)
P 		201.7
(-3)
P 		334
avr 397.7
(2.3) 		374.4
(1.7) 		422.1
(3) 		206.9
(-3) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 354
1 2 3 4 5 6 range
A 183.3
(-3)
P 		348.9
(3)
P 		279.7
(0.5)
P 		297
(1.1)
P 		315.4
(1.8)
P 		275.9
(0.4)
P 		166
B 356
(-2)
P 		393.2
(-1)
P 		320.7
(-3)
P 		357.4
(-2)
P 		524.1
(2.6)
P 		536.9
(3)
P 		216
C 296.2
(-2)
P 		366.8
(-0)
P 		300.6
(-1.9)
P 		262.2
(-3)
P 		309.8
(-1.6)
P 		473.4
(3)
P 		211
avr 278.5
(-3) 		369.6
(0.6) 		300.3
(-2.1) 		305.5
(-1.9) 		383.1
(1.2) 		428.7
(3) 		150
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185040_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2199
Sequence Type Exemplar sequence
Representative Public ID R12E2.12 NCBI
Target Description R12E2.12 /REP_DB=WormBase Gene ID /WP=CE18144 /TR=O61791 /GB=AAC17033.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058917(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058917 NCBI Caenorhabditis elegans R12E2.12 (R12E2.12) mRNA, complete cds. 11/11 None
SNAP00000012683 ENSEMBL cdna:SNAP chromosome:CEL140:I:4173972:4174740:-1 11/11 None
GENEFINDER00000012701 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4173972:4174875:-1 10/11 None
R12E2.12 ENSEMBL cdna:known chromosome:CEL140:I:4173768:4174775:-1 gene:R12E2.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4173971-4174875(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12E2.12
Entrez Gene 187843 Entrez gene
SwissProt O61791 EMBL-EBI
WormBase CE33159 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491318 R12E2.12 [Caenorhabditis elegans] gb|AAC17033.2| Hypothetical protein R12E2.12 [Caenorhabditis elegans] 3.0E-88
blast CAE66739 Hypothetical protein CBG12089 [Caenorhabditis briggsae] 3.0E-85
blast NP_491318 R12E2.12 [Caenorhabditis elegans] gb|AAC17033.2| Hypothetical protein R12E2.12 [Caenorhabditis elegans] 1.0E-80
blast CAE66739 Hypothetical protein CBG12089 [Caenorhabditis briggsae] 1.0E-77
blast AAL03955 28S ribosomal protein S6 precursor [Spodoptera frugiperda] 2.0E-12
Sequence
Target Sequence 
>C. ELEGANS:185040_AT
gatcacacgatctctgtcaaaggcagatcttgttaaagctttaacgcgtgctggaaacac
tttgctggatcacggagcagtaattgaaaaggttgagtcactgggtcatcgagatttacc
gtataaacggctggccaaacagacaaatgagcccgtctacgcttcaaatttcttcctctt
caaggcgcacatgtctcgggaagctagacagaaaacaaagtctattttgacacatgacct
ggacacggttcaagtcgatctaattcaagtggacacactgccggctccaaaggttgaatg
caatctggaagaaatcctcaaatctcctgccgaacgtcaagcagtcaaggatttgagaga
aaatcaaaagatgggtcatttcacgcgacagatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCACACGATCTCTGTCAAAGGCA 652 421 120 Antisense
AAAGCTTTAACGCGTGCTGGAAACA 297 123 154 Antisense
GGAAACACTTTGCTGGATCACGGAG 73 527 172 Antisense
GAGTCACTGGGTCATCGAGATTTAC 99 401 214 Antisense
CAGACAAATGAGCCCGTCTACGCTT 299 205 259 Antisense
GCGCACATGTCTCGGGAAGCTAGAC 678 287 304 Antisense
GACACATGACCTGGACACGGTTCAA 78 367 348 Antisense
ACACGGTTCAAGTCGATCTAATTCA 28 111 362 Antisense
ACACTGCCGGCTCCAAAGGTTGAAT 629 105 394 Antisense
ATCTCCTGCCGAACGTCAAGCAGTC 582 31 441 Antisense
GATGGGTCATTTCACGCGACAGATG 178 409 489 Antisense
Sequence Source Affymetrix Proprietary Database