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[185039_s_at]

Description T25G12.6 /REP_DB=WormBase Gene ID /WP=CE07543 /TR=Q22783 /GB=AAC69024.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 22      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 80      6grp_total > 22      4grp_total > 59      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 374
1 2 3 4 range
A 363.6
(0.3)
P 		513.2
(3)
P 		464.4
(2.1)
P 		181.5
(-3)
P 		332
B 357.8
(0.3)
P 		464.9
(2)
P 		527.5
(3)
P 		153.9
(-3)
P 		374
C 348.4
(0.4)
P 		462.9
(2.7)
P 		476.6
(3)
P 		179.6
(-3)
P 		297
avr 356.6
(0.5) 		480.3
(2.8) 		489.5
(3) 		171.7
(-3) 		318

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 165
1 2 3 4 5 6 range
A 327.9
(3)
P 		263.9
(0.3)
P 		211.2
(-1.9)
P 		223.7
(-1.3)
P 		212.2
(-1.8)
P 		183.8
(-3)
P 		144
B 232.6
(0.2)
P 		272.7
(2)
P 		293.4
(3)
P 		218.8
(-0.4)
P 		192.2
(-1.7)
P 		163
(-3)
P 		130
C 262.1
(1.4)
P 		294.1
(3)
P 		275.6
(2.1)
P 		177.5
(-2.7)
P 		247.7
(0.7)
P 		170.5
(-3)
P 		124
avr 274.2
(2.8) 		276.9
(3) 		260.1
(2) 		206.7
(-1) 		217.4
(-0.4) 		172.4
(-3) 		105
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 132.4
(3)
P 		111.4
(1.8)
P 		121.4
(2.4)
P 		26.6
(-3)
P 		106
B 114.1
(2.5)
P 		114.8
(2.5)
P 		122.2
(3)
P 		32.8
(-3)
P 		89
C 115.3
(0.8)
P 		133.1
(1.5)
P 		166.1
(3)
P 		30.3
(-3)
P 		136
avr 120.6
(2.1) 		119.8
(2.1) 		136.6
(3) 		29.9
(-3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 60.4
(-1.1)
P 		93.9
(3)
P 		46
(-2.8)
P 		56.7
(-1.5)
P 		64
(-0.6)
P 		44.7
(-3)
P 		49
B 80.9
(1.2)
P 		90.8
(3)
P 		71.3
(-0.6)
P 		58
(-3)
P 		78.1
(0.7)
P 		60.8
(-2.5)
P 		33
C 97.1
(3)
P 		89.7
(2.3)
P 		70.2
(0.4)
P 		35.7
(-3)
P 		80.1
(1.3)
P 		57.1
(-0.9)
P 		61
avr 79.5
(1.3) 		91.5
(3) 		62.5
(-1.2) 		50.1
(-3) 		74.1
(0.5) 		54.2
(-2.4) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185039_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17603
Sequence Type Exemplar sequence
Representative Public ID T25G12.6 NCBI
Target Description T25G12.6 /REP_DB=WormBase Gene ID /WP=CE07543 /TR=Q22783 /GB=AAC69024.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078390(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078390 NCBI Caenorhabditis elegans T25G12.6 (T25G12.6) mRNA, complete cds. 11/11 None
SNAP00000027870 ENSEMBL cdna:SNAP chromosome:CEL140:X:17225147:17229960:-1 10/11 None
GENEFINDER00000027882 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17225147:17229960:-1 11/11 None
T25G12.6 ENSEMBL cdna:known chromosome:CEL140:X:17225147:17229960:-1 gene:T25G12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17225146-17229960(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25G12.6
Entrez Gene 181760 Entrez gene
SwissProt Q22783 EMBL-EBI
WormBase CE07543 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69024 Hypothetical protein T25G12.6 [Caenorhabditis elegans] ref|NP_510791.1| T25G12.6 [Caenorhabditis elegans] 0.0
blast CAE69688 Hypothetical protein CBG15943 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002035 EMBL-EBI von Willebrand factor, type A 9.2E-10
Pfam IPR002035 EMBL-EBI von Willebrand factor, type A 1.4E-11
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000027870 1 35-57
GENEFINDER00000027882 1 35-57
Sequence
Target Sequence 
>C. ELEGANS:185039_S_AT
caactagtggtgccacgtcatcagcagctcaaacaactgtagcggttacaactactcaga
ttatgccaagtactacttcgatgccaaatccatgccacgattgttctccaaaaacatcca
atatcctcctcttgttcgaagcatatgggacagctctttcaaatcagaaaaaattagctg
aaactgatcttatcgccacgtggaatcatttcgaaagaacatcagtaatgggatttaata
cactggtgaagaacctggaccccatcaattttggtgacttgcaaaccaaagatgagtttg
tcagcattgttgatagcttcacaccgttcaaggatcagccgagtattgtttcggcattca
atatggctgtcaaccgcgccaacccgctaaaatcctttggaaagatgaactccgtaatct
tcacttctggggcaacacctgctgaaatcactgcatcactgatgaattcacaaactttgc
gacagaatggaaaagtgatcattgtggggcttcaacttccggacgctacacacctggaca
cgctttgtgatgttctcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACTAGTGGTGCCACGTCATCAGC 467 187 1703 Antisense
GCCAAATCCATGCCACGATTGTTCT 323 279 1785 Antisense
AAAAACATCCAATATCCTCCTCTTG 102 131 1812 Antisense
TATCCTCCTCTTGTTCGAAGCATAT 593 657 1824 Antisense
GCTGAAACTGATCTTATCGCCACGT 43 297 1879 Antisense
ACTGGTGAAGAACCTGGACCCCATC 7 97 1944 Antisense
GATAGCTTCACACCGTTCAAGGATC 266 423 2014 Antisense
TGAACTCCGTAATCTTCACTTCTGG 308 571 2108 Antisense
ACCTGCTGAAATCACTGCATCACTG 535 87 2139 Antisense
TGATCATTGTGGGGCTTCAACTTCC 442 565 2198 Antisense
TGGACACGCTTTGTGATGTTCTCCT 103 549 2237 Antisense
Sequence Source Affymetrix Proprietary Database