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[185038_at]

Description F09E5.13 /REP_DB=WormBase Gene ID /WP=CE02608 /TR=Q19263 /GB=AAA79353.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 96      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 91      6grp_total > 86      4grp_total > 31      6grp_highest_sub > In      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 162.9
(-0.5)
P 		175.8
(1.1)
P 		143.8
(-3)
P 		190.5
(3)
P 		47
B 162.2
(0.8)
P 		142.8
(-1.2)
P 		182.5
(3)
P 		126
(-3)
P 		57
C 232.9
(3)
P 		78.6
(-3)
P 		104.6
(-2)
P 		161.1
(0.2)
P 		154
avr 186
(3) 		132.4
(-3) 		143.6
(-1.7) 		159.2
(-0) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 185
1 2 3 4 5 6 range
A 96.1
(-3)
P 		110.9
(-2.4)
P 		118.9
(-2.1)
P 		130.7
(-1.6)
P 		207.3
(1.4)
P 		249.4
(3)
P 		153
B 156.5
(-0.7)
P 		93.5
(-3)
P 		179.9
(0.2)
P 		172.5
(-0.1)
P 		167.5
(-0.3)
P 		257.7
(3)
P 		164
C 128.3
(-1.9)
P 		95.6
(-3)
P 		148.9
(-1.3)
P 		182.4
(-0.2)
P 		172.3
(-0.5)
P 		278.5
(3)
P 		183
avr 127
(-2) 		100
(-3) 		149.2
(-1.2) 		161.9
(-0.7) 		182.4
(0.1) 		261.9
(3) 		162
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 59.3
(3)
P 		38.2
(-1)
P 		37.6
(-1.1)
P 		27.9
(-3)
P 		31
B 51.7
(3)
P 		35.3
(-1)
P 		42.3
(0.7)
P 		26.8
(-3)
P 		25
C 77.1
(3)
P 		22.6
(-3)
P 		36.4
(-1.5)
P 		27.2
(-2.5)
P 		55
avr 62.7
(3) 		32
(-2.2) 		38.8
(-1.1) 		27.3
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 17.7
(-3)
P 		39.4
(-0.1)
P 		25.9
(-1.9)
P 		33.1
(-0.9)
P 		62.6
(3)
P 		60.7
(2.7)
P 		45
B 54.5
(-0.8)
P 		31.1
(-3)
P 		43.7
(-1.8)
P 		45.7
(-1.7)
P 		68.1
(0.4)
P 		96.1
(3)
P 		65
C 47.6
(-1.3)
P 		29.2
(-3)
P 		37.9
(-2.2)
P 		36.7
(-2.3)
P 		55.7
(-0.5)
P 		93.2
(3)
P 		64
avr 39.9
(-2.2) 		33.2
(-3) 		35.8
(-2.7) 		38.5
(-2.4) 		62.1
(0.5) 		83.3
(3) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185038_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4977
Sequence Type Exemplar sequence
Representative Public ID F09E5.13 NCBI
Target Description F09E5.13 /REP_DB=WormBase Gene ID /WP=CE02608 /TR=Q19263 /GB=AAA79353.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062607(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062607 NCBI Caenorhabditis elegans AlkylGuanine DNA alkylTransferase family member (agt-2) (agt-2) mRNA, complete cds. 11/11 None
SNAP00000044018 ENSEMBL cdna:SNAP chromosome:CEL140:II:5372107:5373316:-1 11/11 None
GENEFINDER00000044032 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5372107:5373316:-1 11/11 None
F09E5.13 ENSEMBL cdna:known chromosome:CEL140:II:5372107:5373316:-1 gene:F09E5.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5372104-5373314(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol agt-2
Ensembl F09E5.13
Entrez Gene 184244 Entrez gene
SwissProt Q19263 EMBL-EBI
WormBase CE02608 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA79353 Alkylguanine dna alkyltransferase protein 2 [Caenorhabditis elegans] ref|NP_495008.1| AlkylGuanine DNA alkylTransferase family member (agt-2) [Caenorhabditis elegans] 1.0E-139
Sequence
Target Sequence 
>C. ELEGANS:185038_AT
tgtacaaccttgtcacccagtaagacaaccacgcgaaagcttttactcatctcatgaaca
tgatccaatggatgaagttttactacaagaagtgaaggaagaagctcattcactggcttt
caaaggaataccaattgaaaccaagggtcctacaccatcatcaactattatgaatcctga
agttacgcttagagacgttctgacgtttcacaaaaatattaaaagaagaccacaagagac
aaaaccggcaaaatttgatttctccgcaatgccatcaacttctcgacttccacctacatc
tccaaaacgctaccgccagtttgcacctcctccaatctctatgaaacagtctcccaaaat
ttgtgccaaaactgtttcgtgtccgagtacgtccgaagtaaagagcccacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTACAACCTTGTCACCCAGTAAGA 549 557 210 Antisense
TCACCCAGTAAGACAACCACGCGAA 271 625 222 Antisense
GCGAAAGCTTTTACTCATCTCATGA 526 289 242 Antisense
GAAGAAGCTCATTCACTGGCTTTCA 128 341 307 Antisense
TGAAACCAAGGGTCCTACACCATCA 247 573 345 Antisense
GTCCTACACCATCATCAACTATTAT 317 477 356 Antisense
GTTACGCTTAGAGACGTTCTGACGT 555 443 391 Antisense
AAATTTGATTTCTCCGCAATGCCAT 102 117 460 Antisense
CAATCTCTATGAAACAGTCTCCCAA 529 181 542 Antisense
TGTTTCGTGTCCGAGTACGTCCGAA 372 559 582 Antisense
TACGTCCGAAGTAAAGAGCCCACCA 424 569 597 Antisense
Sequence Source Affymetrix Proprietary Database