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[185016_at]

Description T28F12.1 /REP_DB=WormBase Gene ID /WP=CE21218 /TR=Q9N5D4 /GB=AAF39973.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 17      4grp_total > 34      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 32.8
(-3)
P 		49.8
(0.8)
A 		59.6
(3)
A 		36.4
(-2.2)
A 		27
B 14
(-3)
A 		42.2
(3)
A 		36.5
(1.8)
A 		24.1
(-0.9)
A 		28
C 44.3
(-1)
P 		56.8
(0.9)
P 		31.3
(-3)
M 		71
(3)
P 		40
avr 30.4
(-3) 		49.6
(3) 		42.5
(0.8) 		43.8
(1.2) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 67.9
(3)
M 		43.2
(-0.5)
P 		27.7
(-2.7)
A 		25.8
(-3)
A 		37.5
(-1.3)
A 		30.1
(-2.4)
A 		42
B 41.4
(-2.2)
M 		55.8
(1.9)
P 		48.5
(-0.2)
A 		42.9
(-1.8)
A 		38.6
(-3)
A 		59.8
(3)
P 		21
C 44.3
(3)
A 		32.8
(-1.5)
A 		43.2
(2.6)
A 		41.9
(2.1)
A 		38.1
(0.6)
M 		29.1
(-3)
P 		15
avr 51.2
(3) 		43.9
(-0.1) 		39.8
(-1.8) 		36.9
(-3) 		38.1
(-2.5) 		39.7
(-1.8) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 12
(0.9)
P 		10.8
(0.2)
A 		15.6
(3)
A 		5.3
(-3)
A 		10
B 4.5
(-3)
A 		10.4
(3)
A 		8.5
(1.1)
A 		5.1
(-2.4)
A 		6
C 14.7
(1.2)
P 		16.3
(3)
P 		10.9
(-3)
M 		12
(-1.8)
P 		5
avr 10.4
(0.5) 		12.5
(3) 		11.7
(2) 		7.5
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 12.5
(1.1)
M 		15.4
(3)
P 		6
(-3)
A 		6.5
(-2.7)
A 		11.3
(0.4)
A 		7.3
(-2.2)
A 		9
B 14.4
(-1.3)
M 		18.6
(1)
P 		11.8
(-2.8)
A 		11.4
(-3)
A 		15.7
(-0.6)
A 		22.3
(3)
P 		11
C 16.4
(3)
A 		10
(-1.8)
A 		11
(-1.1)
A 		8.4
(-3)
A 		12.3
(-0.1)
M 		9.7
(-2)
P 		8
avr 14.4
(2.7) 		14.7
(3) 		9.6
(-2.2) 		8.8
(-3) 		13.1
(1.4) 		13.1
(1.4) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185016_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15946
Sequence Type Exemplar sequence
Representative Public ID T28F12.1 NCBI
Target Description T28F12.1 /REP_DB=WormBase Gene ID /WP=CE21218 /TR=Q9N5D4 /GB=AAF39973.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071833(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071833 NCBI Caenorhabditis elegans T28F12.1 (T28F12.1) mRNA, complete cds. 11/11 None
SNAP00000005052 ENSEMBL cdna:SNAP chromosome:CEL140:V:4511324:4516761:1 11/11 None
GENEFINDER00000005061 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4512966:4516761:1 11/11 None
T28F12.1 ENSEMBL cdna:known chromosome:CEL140:V:4511791:4516761:1 gene:T28F12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4512957-4516761(+) 93.83 93.9
Public Domain and Genome References
Gene Title
Ensembl T28F12.1
Entrez Gene 189060 Entrez gene
SwissProt Q9N5D4 EMBL-EBI
WormBase CE21218 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39973 Hypothetical protein T28F12.1 [Caenorhabditis elegans] ref|NP_504234.1| T28F12.1 [Caenorhabditis elegans] 0.0
blast AAF39973 Hypothetical protein T28F12.1 [Caenorhabditis elegans] ref|NP_504234.1| T28F12.1 [Caenorhabditis elegans] 1.0E-159
blast AAF39973 Hypothetical protein T28F12.1 [Caenorhabditis elegans] ref|NP_504234.1| T28F12.1 [Caenorhabditis elegans] 1.0E-111
blast AAK68558 Hypothetical protein Y69A2AR.14 [Caenorhabditis elegans] ref|NP_500220.1| Y69A2AR.14 [Caenorhabditis elegans] 1.0E-18
blast AAK68558 Hypothetical protein Y69A2AR.14 [Caenorhabditis elegans] ref|NP_500220.1| Y69A2AR.14 [Caenorhabditis elegans] 2.0E-18
blast AAF59585 Hypothetical protein Y54G2A.13 [Caenorhabditis elegans] ref|NP_500256.2| Y54G2A.13 [Caenorhabditis elegans] 1.0E-15
blast AAF59585 Hypothetical protein Y54G2A.13 [Caenorhabditis elegans] ref|NP_500256.2| Y54G2A.13 [Caenorhabditis elegans] 2.0E-15
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504234.1 2 47-66,158-180
T28F12.1 2 47-66,158-180
SNAP00000005052 1 19-41
GENEFINDER00000005061 2 20-37,107-129
Sequence
Target Sequence 
>C. ELEGANS:185016_AT
gatgcccagcaaaagtcggacactagaagtgacaagttcatcaagagagggaagatttat
attccgattgagacacatatcccaccagcgtccgtccttccgaacaacgtcattctggac
aaagtcacatataatccgagtgccaacgaaattttcgacattggatccgacattgacgac
atcgaaatggagccaagtacgatgcagggctcggatagtggtggtgatccaactgacaaa
tcgattgctgataaaactccgaaaaaggaagtaaaagttgaaacgccgaagaaggatgga
aatatggcaaaggcttccccgaaagtaaagaaaactccagtaaagactccagggaagcag
ccgggaagatctccagcaaaacccgggatggaagatgtaacgcagttgagccaggaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCCCAGCAAAAGTCGGACACTA 698 403 826 Antisense
CCGATTGAGACACATATCCCACCAG 156 265 889 Antisense
TAATCCGAGTGCCAACGAAATTTTC 492 635 957 Antisense
GACATTGGATCCGACATTGACGACA 212 371 982 Antisense
GATGCAGGGCTCGGATAGTGGTGGT 352 413 1026 Antisense
GGTGGTGATCCAACTGACAAATCGA 192 499 1045 Antisense
ATATGGCAAAGGCTTCCCCGAAAGT 510 21 1127 Antisense
AAAGACTCCAGGGAAGCAGCCGGGA 341 123 1167 Antisense
GGAAGCAGCCGGGAAGATCTCCAGC 93 531 1178 Antisense
AGATCTCCAGCAAAACCCGGGATGG 292 65 1192 Antisense
GATGTAACGCAGTTGAGCCAGGAGA 549 409 1219 Antisense
Sequence Source Affymetrix Proprietary Database