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[184997_at]

Description F28A12.1 /REP_DB=WormBase Gene ID /WP=CE09735 /TR=Q22970 /GB=AAC47987.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 05      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 45.7
(0.5)
A 		27.5
(-3)
A 		34.6
(-1.6)
A 		58.9
(3)
P 		31
B 25.7
(2.3)
A 		1.9
(-3)
A 		24.6
(2)
A 		28.9
(3)
A 		27
C 34.6
(0.1)
A 		28.2
(-0.6)
A 		5.9
(-3)
A 		60.8
(3)
P 		55
avr 35.3
(0.2) 		19.2
(-3) 		21.7
(-2.5) 		49.5
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 4.8
(-3)
A 		24.4
(-0.3)
A 		48.8
(3)
A 		44
(2.3)
A 		5.2
(-2.9)
A 		31.1
(0.6)
A 		44
B 29
(1.2)
A 		28.4
(1.1)
P 		31
(1.8)
A 		17.2
(-1.9)
A 		35.6
(3)
A 		13
(-3)
A 		23
C 12.5
(-3)
A 		34.7
(3)
P 		26.8
(0.9)
A 		15.4
(-2.2)
A 		15.3
(-2.2)
A 		18.8
(-1.3)
A 		22
avr 15.4
(-3) 		29.2
(1.1) 		35.5
(3) 		25.5
(0) 		18.7
(-2) 		21
(-1.3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 16.6
(3)
A 		6
(-3)
A 		9
(-1.3)
A 		8.6
(-1.5)
P 		11
B 8.2
(3)
A 		0.5
(-3)
A 		5.7
(1.1)
A 		6.2
(1.4)
A 		8
C 11.4
(3)
A 		8.1
(0.9)
A 		2.1
(-3)
A 		10.3
(2.3)
P 		9
avr 12.1
(3) 		4.9
(-3) 		5.6
(-2.4) 		8.4
(-0.1) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 0.9
(-3)
A 		8.7
(1.5)
A 		10.6
(2.7)
A 		11.2
(3)
A 		1.6
(-2.6)
A 		7.6
(0.9)
A 		10
B 10.1
(0.4)
A 		9.4
(-0.1)
P 		7.5
(-1.2)
A 		4.6
(-3)
A 		14.4
(3)
A 		4.8
(-2.9)
A 		10
C 4.6
(-1.8)
A 		10.6
(3)
P 		6.8
(-0)
A 		3.1
(-3)
A 		5
(-1.5)
A 		6.3
(-0.4)
A 		8
avr 5.2
(-3) 		9.6
(3) 		8.3
(1.2) 		6.3
(-1.5) 		7
(-0.5) 		6.2
(-1.6) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184997_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11734
Sequence Type Exemplar sequence
Representative Public ID F28A12.1 NCBI
Target Description F28A12.1 /REP_DB=WormBase Gene ID /WP=CE09735 /TR=Q22970 /GB=AAC47987.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072829(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072829 NCBI Caenorhabditis elegans F28A12.1 (F28A12.1) mRNA, complete cds. 11/11 None
GENEFINDER00000012222 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8640842:8643173:1 11/11 None
F28A12.1 ENSEMBL cdna:known chromosome:CEL140:V:8640842:8643173:1 gene:F28A12.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000012217 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8640842-8643174(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28A12.1
Entrez Gene 185036 Entrez gene
SwissProt Q22970 EMBL-EBI
WormBase CE09735 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC47987 Hypothetical protein F28A12.1 [Caenorhabditis elegans] ref|NP_505230.1| F28A12.1 [Caenorhabditis elegans] 0.0
blast CAE58344 Hypothetical protein CBG01465 [Caenorhabditis briggsae] 0.0
blast AAB42340 Hypothetical protein C24G7.4 [Caenorhabditis elegans] ref|NP_491293.1| C24G7.4 [Caenorhabditis elegans] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505230.1 2 60-82,472-494
F28A12.1 2 60-82,472-494
GENEFINDER00000012222 2 60-82,472-494
Sequence
Target Sequence 
>C. ELEGANS:184997_AT
atacacatcttatggtgacggatttaattataattcaatgaaatggcttacaagagaaac
caatcgcagcgcgagttacattagacaaaatatagcaataataaatattcattttatgga
attgttctacacttcatactctcaagttaaagcaacaactattttgaatactttcaataa
aatatttggtttgaacggtttgtggtttggaatgtcagttgtttctttaactgagctaat
tctatacttcacgaaaatctcgtggatcgctgtatcaagtaagcgaaggcaatatctttt
tgagaagaaaatgtcggaaaagagaaaggaaagaaatatcgaagaagctgtacaagaagc
tgaaatatctagatcacgaagaagctcagctgcaaatttgaaatttctggatattgaaag
tttagatgatgaagattactggtacagaagctcgagccaactttcggatagccatttgga
caatgtgattcaactcgctattgactttgaaaaacccct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATACACATCTTATGGTGACGGATTT 180 25 1227 Antisense
ACAAGAGAAACCAATCGCAGCGCGA 446 113 1276 Antisense
ATCGCAGCGCGAGTTACATTAGACA 268 37 1289 Antisense
AATTGTTCTACACTTCATACTCTCA 298 179 1346 Antisense
GGTTTGGAATGTCAGTTGTTTCTTT 89 501 1430 Antisense
AACTGAGCTAATTCTATACTTCACG 194 143 1455 Antisense
AAATCTCGTGGATCGCTGTATCAAG 687 117 1481 Antisense
GGTACAGAAGCTCGAGCCAACTTTC 377 503 1667 Antisense
GAGCCAACTTTCGGATAGCCATTTG 301 385 1680 Antisense
GGACAATGTGATTCAACTCGCTATT 44 527 1704 Antisense
TCGCTATTGACTTTGAAAAACCCCT 322 417 1721 Antisense
Sequence Source Affymetrix Proprietary Database