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[184986_at]

Description T20D4.15 /REP_DB=WormBase Gene ID /WP=CE13806 /TR=P91460 /GB=AAB37582.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 75      4grp_total > 16      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 63.1
(-0.7)
A 		57.9
(-3)
A 		71.3
(3)
A 		61.5
(-1.4)
A 		13
B 18.7
(-3)
A 		39.2
(-2.1)
A 		134.7
(2.2)
A 		151.3
(3)
A 		133
C 47.7
(-3)
A 		82.5
(-1.4)
A 		66.3
(-2.1)
A 		178.2
(3)
P 		131
avr 43.2
(-3) 		59.9
(-1.8) 		90.8
(0.3) 		130.3
(3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 21.9
(-2.2)
A 		46.9
(1.2)
A 		17.9
(-2.7)
A 		57.4
(2.6)
A 		15.7
(-3)
A 		60.1
(3)
A 		44
B 16.5
(-3)
A 		18.5
(-2.7)
A 		16.8
(-3)
A 		36.5
(-0.2)
A 		18.3
(-2.8)
A 		59.7
(3)
A 		43
C 23.3
(-2.5)
A 		19.7
(-2.8)
A 		18.3
(-2.9)
A 		97.9
(3)
A 		16.4
(-3)
A 		74.9
(1.3)
A 		82
avr 20.6
(-2.5) 		28.4
(-1.6) 		17.7
(-2.9) 		63.9
(2.9) 		16.8
(-3) 		64.9
(3) 		48
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 23
(3)
A 		12.6
(-1.5)
A 		18.6
(1.1)
A 		9
(-3)
A 		14
B 6
(-3)
A 		9.7
(-2.2)
A 		31.2
(2.8)
A 		32.2
(3)
A 		26
C 15.8
(-3)
A 		23.7
(0.3)
A 		23.1
(0.1)
A 		30.1
(3)
P 		14
avr 14.9
(-3) 		15.3
(-2.7) 		24.3
(3) 		23.8
(2.7) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 4
(-3)
A 		16.7
(3)
A 		3.9
(-3)
A 		14.5
(2)
A 		4.7
(-2.6)
A 		14.6
(2)
A 		13
B 5.7
(-2.5)
A 		6.1
(-2.3)
A 		4.1
(-3)
A 		9.7
(-1.2)
A 		7.4
(-1.9)
A 		22.3
(3)
A 		18
C 8.6
(-1.8)
A 		6
(-2.6)
A 		4.6
(-3)
A 		19.7
(1.4)
A 		5.3
(-2.8)
A 		25.1
(3)
A 		21
avr 6.1
(-2.3) 		9.6
(-1) 		4.2
(-3) 		14.6
(0.8) 		5.8
(-2.4) 		20.7
(3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184986_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15303
Sequence Type Exemplar sequence
Representative Public ID T20D4.15 NCBI
Target Description T20D4.15 /REP_DB=WormBase Gene ID /WP=CE13806 /TR=P91460 /GB=AAB37582.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071545(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071545 NCBI Caenorhabditis elegans T20D4.15 (T20D4.15) mRNA, complete cds. 11/11 None
SNAP00000008842 ENSEMBL cdna:SNAP chromosome:CEL140:V:3393412:3393795:-1 9/11 None
GENEFINDER00000008856 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3393325:3394514:-1 11/11 None
T20D4.15 ENSEMBL cdna:known chromosome:CEL140:V:3393302:3393831:-1 gene:T20D4.15 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3393301-3393831(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D4.15
Entrez Gene 188650 Entrez gene
SwissProt P91460 EMBL-EBI
WormBase CE13806 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99928 Hypothetical protein F35F10.13 [Caenorhabditis elegans] ref|NP_503919.1| F35F10.13 [Caenorhabditis elegans] 3.0E-95
blast AAB37582 Hypothetical protein T20D4.15 [Caenorhabditis elegans] ref|NP_503946.1| T20D4.15 [Caenorhabditis elegans] 1.0E-87
blast AAB37582 Hypothetical protein T20D4.15 [Caenorhabditis elegans] ref|NP_503946.1| T20D4.15 [Caenorhabditis elegans] 3.0E-78
blast AAB37582 Hypothetical protein T20D4.15 [Caenorhabditis elegans] ref|NP_503946.1| T20D4.15 [Caenorhabditis elegans] 7.0E-71
blast AAF99928 Hypothetical protein F35F10.13 [Caenorhabditis elegans] ref|NP_503919.1| F35F10.13 [Caenorhabditis elegans] 2.0E-65
blast AAF99928 Hypothetical protein F35F10.13 [Caenorhabditis elegans] ref|NP_503919.1| F35F10.13 [Caenorhabditis elegans] 2.0E-59
blast NP_503904 T28A11.3 [Caenorhabditis elegans] gb|AAC48115.1| Hypothetical protein T28A11.3 [Caenorhabditis elegans] 1.0E-36
blast T25496 hypothetical protein C03G6.5 - Caenorhabditis elegans 1.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 2.5E-33
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 4.6E-31
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 3.8E-39
Sequence
Target Sequence 
>C. ELEGANS:184986_AT
atgcacgatttacccttgaaaatgaacgaactcaacatgaatgacataaatgagatgagg
gaattcaagaaatatgtggacaatgcgatcaactgtatgactgctctcacatgcccgcca
tacaccaaggatgcgaaaaaacaggggattgagctaactggaaagtatcgccaaactctt
gtgtattttgccgatgagttcccccagtgtagtgaaaagttgaatgctagaaaatcaaag
tgtttcgaccactgggacctcaataaaattcgcgctacagatgaggaaaaggaaaatcag
cacaaaagaagtgtgacttgtgagaaatatttcggaaaaggtgactgtttgaagaacgaa
attacggaggcgtgtggcaagaaagaatggaataagtttcgtgagtacatgcgtattcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCACGATTTACCCTTGAAAATGA 274 41 13 Antisense
CGATCAACTGTATGACTGCTCTCAC 329 249 98 Antisense
TGCCCGCCATACACCAAGGATGCGA 177 585 124 Antisense
AGGGGATTGAGCTAACTGGAAAGTA 504 55 155 Antisense
GGAAAGTATCGCCAAACTCTTGTGT 649 527 172 Antisense
GCCAAACTCTTGTGTATTTTGCCGA 370 277 182 Antisense
ATGAGTTCCCCCAGTGTAGTGAAAA 487 43 206 Antisense
GTTTCGACCACTGGGACCTCAATAA 302 451 254 Antisense
GGGACCTCAATAAAATTCGCGCTAC 650 493 266 Antisense
GAAATTACGGAGGCGTGTGGCAAGA 473 361 370 Antisense
GTTTCGTGAGTACATGCGTATTCAA 105 451 408 Antisense
Sequence Source Affymetrix Proprietary Database