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[184975_at]

Description C34B2.10 /REP_DB=WormBase Gene ID /WP=CE16898 /TR=O44953 /GB=AAB97532.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 45      6grp_total > 81      4grp_total > 49      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1548
1 2 3 4 range
A 1721
(-3)
P 		3268.7
(3)
P 		2762.1
(1)
P 		2250.3
(-0.9)
P 		1548
B 1736.3
(-3)
P 		2925.5
(2.4)
P 		3055
(3)
P 		1943.8
(-2.1)
P 		1319
C 1774.9
(-3)
P 		2717.8
(2.8)
P 		2755.3
(3)
P 		1840.8
(-2.6)
P 		980
avr 1744.1
(-3) 		2970.7
(3) 		2857.5
(2.4) 		2011.6
(-1.7) 		1227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1860
1 2 3 4 5 6 range
A 2571.2
(-0.3)
P 		2336.2
(-1)
P 		2763.5
(0.4)
P 		3580.8
(3)
P 		2748.4
(0.3)
P 		1720.7
(-3)
P 		1860
B 2288.4
(-0.9)
P 		2602.5
(0.6)
P 		2785.7
(1.4)
P 		3144.8
(3)
P 		2825.1
(1.6)
P 		1813.9
(-3)
P 		1331
C 2313
(-1.3)
P 		2732.5
(0.3)
P 		2269.2
(-1.5)
P 		3472.6
(3)
P 		2525.5
(-0.5)
P 		1857
(-3)
P 		1616
avr 2390.9
(-0.8) 		2557.1
(-0.2) 		2606.1
(0) 		3399.4
(3) 		2699.7
(0.4) 		1797.2
(-3) 		1602
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 650
1 2 3 4 range
A 626.6
(1.5)
P 		709.5
(2.8)
P 		722.1
(3)
P 		329.4
(-3)
P 		393
B 553.7
(-0.3)
P 		722.5
(3)
P 		707.9
(2.7)
P 		414.2
(-3)
P 		308
C 587.5
(-0.4)
P 		781.4
(1.3)
P 		960.3
(3)
P 		310.4
(-3)
P 		650
avr 589.3
(0.2) 		737.8
(2.2) 		796.8
(3) 		351.3
(-3) 		446

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 729
1 2 3 4 5 6 range
A 474
(-2.3)
P 		830.9
(2.1)
P 		601.8
(-0.8)
P 		908.2
(3)
P 		829.4
(2)
P 		418.9
(-3)
P 		489
B 796.3
(-1.5)
P 		866.3
(-0.6)
P 		676.5
(-3)
P 		833
(-1)
P 		1147.8
(3)
P 		676.4
(-3)
P 		471
C 857.3
(3)
P 		833.8
(2.5)
P 		577.6
(-3)
P 		698.3
(-0.4)
P 		817.2
(2.1)
P 		621.7
(-2.1)
P 		280
avr 709.2
(-0.7) 		843.7
(1.5) 		618.6
(-2.2) 		813.2
(1) 		931.5
(3) 		572.3
(-3) 		359
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184975_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2451
Sequence Type Exemplar sequence
Representative Public ID C34B2.10 NCBI
Target Description C34B2.10 /REP_DB=WormBase Gene ID /WP=CE16898 /TR=O44953 /GB=AAB97532.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060396(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060396 NCBI Caenorhabditis elegans C34B2.10 (C34B2.10) mRNA, complete cds. 11/11 None
SNAP00000007246 ENSEMBL cdna:SNAP chromosome:CEL140:I:10690743:10691161:-1 11/11 None
GENEFINDER00000007257 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10690743:10691161:-1 11/11 None
C34B2.10.2 ENSEMBL cdna:known chromosome:CEL140:I:10690596:10691169:-1 gene:C34B2.10 11/11 None
C34B2.10.1 ENSEMBL cdna:known chromosome:CEL140:I:10690621:10691243:-1 gene:C34B2.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10690673-10691092(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C34B2.10
Entrez Gene 172967 Entrez gene
SwissProt O44953 EMBL-EBI
WormBase CE16898 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB97532 Hypothetical protein C34B2.10 [Caenorhabditis elegans] ref|NP_492797.1| C34B2.10 [Caenorhabditis elegans] sp|O44953|SPCS1_CAEEL Probable signal peptidase complex subunit 1 (Microsomal signal peptidase 12 kDa subunit) (SPase 12 kDa subunit) 3.0E-50
blast Q61CQ8 Probable signal peptidase complex subunit 1 (Microsomal signal peptidase 12 kDa subunit) (SPase 12 kDa subunit) emb|CAE67341.1| Hypothetical protein CBG12804 [Caenorhabditis briggsae] 1.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR009542 EMBL-EBI Microsomal signal peptidase 12 kDa subunit 1.8E-51
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492797.1 2 33-52,56-78
C34B2.10.2 2 33-52,56-78
C34B2.10.1 2 33-52,56-78
SNAP00000007246 2 33-52,56-78
GENEFINDER00000007257 2 33-52,56-78
Sequence
Target Sequence 
>C. ELEGANS:184975_AT
cggaatgattgcaatgctccctgctccactgcagaaactatcgtcgcatattgattttca
aggacagaaagtcgccgagagaacttaccaggtaatcctcactattgctggaattattgg
cttcctcgttggattctggactcaacagctttcatatgccatgttcacagtgctgggagc
atctgcgttcacagctctcatcattcttccaccatggccattcctcttcagaaagaaccc
gattgtgtggcacac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGAATGATTGCAATGCTCCCTGCT 322 243 18 Antisense
GCTCCACTGCAGAAACTATCGTCGC 10 299 40 Antisense
GTCGCCGAGAGAACTTACCAGGTAA 185 475 88 Antisense
TACCAGGTAATCCTCACTATTGCTG 139 651 103 Antisense
GCTGGAATTATTGGCTTCCTCGTTG 421 293 124 Antisense
CTTCCTCGTTGGATTCTGGACTCAA 632 219 138 Antisense
GGACTCAACAGCTTTCATATGCCAT 247 525 155 Antisense
ATGCCATGTTCACAGTGCTGGGAGC 185 41 173 Antisense
GCTGGGAGCATCTGCGTTCACAGCT 519 295 189 Antisense
CATGGCCATTCCTCTTCAGAAAGAA 348 209 230 Antisense
GAAAGAACCCGATTGTGTGGCACAC 100 361 248 Antisense
Sequence Source Affymetrix Proprietary Database