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[184945_at]

Description Y54H5A.1 /REP_DB=WormBase Gene ID /WP=CE27292 /TR=Q9N393 /GB=AAF60780.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 95      4grp_highest_8_8 > 30      4grp_high_8_8 > 07      6grp_highest_nonorm > 78      6grp_total > 86      4grp_total > 01      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2839
1 2 3 4 range
A 1976.4
(-1.7)
P 		1496.8
(-3)
P 		1863
(-2)
P 		3645.5
(3)
P 		2149
B 2119.9
(-2)
P 		1687.9
(-3)
P 		1965
(-2.4)
P 		4336
(3)
P 		2648
C 2048.4
(-1.7)
P 		1542.6
(-3)
P 		1976.3
(-1.9)
P 		3912.2
(3)
P 		2370
avr 2048.2
(-1.8) 		1575.8
(-3) 		1934.8
(-2.1) 		3964.6
(3) 		2389

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1490
1 2 3 4 5 6 range
A 916.7
(-3)
P 		1158.8
(-1.3)
P 		1405.7
(0.5)
P 		1429.1
(0.6)
P 		1677.5
(2.4)
P 		1763.7
(3)
P 		847
B 1021.1
(-3)
P 		1382.2
(-1.4)
P 		1678.6
(-0.1)
P 		1618.1
(-0.4)
P 		1861.2
(0.6)
P 		2402.1
(3)
P 		1381
C 1062.3
(-3)
P 		1374.2
(-1.6)
P 		1139.9
(-2.7)
P 		1819.1
(0.4)
P 		1268.8
(-2.1)
P 		2406.5
(3)
P 		1344
avr 1000
(-3) 		1305.1
(-1.5) 		1408.1
(-0.9) 		1622.1
(0.1) 		1602.5
(0) 		2190.8
(3) 		1191
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 599
1 2 3 4 range
A 719.6
(3)
P 		324.9
(-3)
P 		487
(-0.5)
P 		533.6
(0.2)
P 		395
B 676
(0.1)
P 		416.9
(-3)
P 		455.3
(-2.5)
P 		924
(3)
P 		507
C 678.1
(2.7)
P 		443.5
(-3)
P 		688.8
(3)
P 		659.7
(2.3)
P 		245
avr 691.2
(2.7) 		395.1
(-3) 		543.7
(-0.1) 		705.8
(3) 		311

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 727
1 2 3 4 5 6 range
A 169
(-3)
P 		412.1
(1.3)
P 		306.1
(-0.6)
P 		362.5
(0.4)
P 		506.2
(3)
P 		429.4
(1.6)
P 		337
B 355.3
(-3)
P 		460.1
(-1.8)
P 		407.6
(-2.4)
P 		428.6
(-2.2)
P 		756.2
(1.5)
P 		895.7
(3)
P 		540
C 393.7
(-1.8)
P 		419.3
(-1.5)
P 		290.1
(-3)
P 		365.8
(-2.1)
P 		410.6
(-1.6)
P 		805.6
(3)
P 		516
avr 306
(-3) 		430.5
(-1.2) 		334.6
(-2.6) 		385.6
(-1.8) 		557.7
(0.7) 		710.2
(3) 		404
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184945_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7463
Sequence Type Exemplar sequence
Representative Public ID Y54H5A.1 NCBI
Target Description Y54H5A.1 /REP_DB=WormBase Gene ID /WP=CE27292 /TR=Q9N393 /GB=AAF60780.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065690(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065690 NCBI Caenorhabditis elegans Y54H5A.1 (Y54H5A.1) mRNA, complete cds. 11/11 None
SNAP00000038484 ENSEMBL cdna:SNAP chromosome:CEL140:III:5153503:5155273:1 11/11 None
GENEFINDER00000038487 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5153503:5155273:1 11/11 None
Y54H5A.1.2 ENSEMBL cdna:known chromosome:CEL140:III:5153421:5155381:1 gene:Y54H5A.1 11/11 None
Y54H5A.1.1 ENSEMBL cdna:known chromosome:CEL140:III:5153500:5155381:1 gene:Y54H5A.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5153501-5155272(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54H5A.1
Entrez Gene 175701 Entrez gene
SwissProt Q9N393 EMBL-EBI
WormBase CE27292 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13630_AT transducin family protein / WD-40 repeat family protein at
ATH1-121501:265947_AT transducin family protein / WD-40 repeat family protein at
CANINE_2:CFAAFFX.6922.1.S1_S_AT similar to glutamate-rich WD repeat containing 1 (predicted) cfa
DROSOPHILA_2:1639392_AT dm
DROSGENOME1:146653_AT dm
HG-U133_PLUS_2:221549_AT glutamate-rich WD repeat containing 1 hs
HG-U133A:221549_AT glutamate-rich WD repeat containing 1 hs
HG-U133A_2:221549_AT glutamate-rich WD repeat containing 1 hs
HU35KSUBB:RC_Z39055_AT glutamate-rich WD repeat containing 1 hs
U133_X3P:G13274610_3P_AT glutamate-rich WD repeat containing 1 hs
HG-U95C:61622_I_AT glutamate-rich WD repeat containing 1 hs
HG-U95C:61623_R_AT glutamate-rich WD repeat containing 1 hs
HG-U95E:71517_AT glutamate-rich WD repeat containing 1 hs
MU11KSUBA:AA198324_AT glutamate-rich WD repeat containing 1 mm
MG-U74AV2:103853_AT glutamate-rich WD repeat containing 1 mm
MG-U74AV2:161421_R_AT glutamate-rich WD repeat containing 1 mm
MG-U74CV2:171390_I_AT glutamate-rich WD repeat containing 1 mm
MG-U74CV2:171003_R_AT glutamate-rich WD repeat containing 1 mm
MOE430A:1452171_AT glutamate-rich WD repeat containing 1 mm
MOUSE430A_2:1452171_AT glutamate-rich WD repeat containing 1 mm
MOUSE430_2:1452171_AT glutamate-rich WD repeat containing 1 mm
MOE430A:1436887_X_AT glutamate-rich WD repeat containing 1 mm
MOUSE430A_2:1436887_X_AT glutamate-rich WD repeat containing 1 mm
MOUSE430_2:1436887_X_AT glutamate-rich WD repeat containing 1 mm
MOE430A:1455841_S_AT glutamate-rich WD repeat containing 1 mm
MOUSE430A_2:1455841_S_AT glutamate-rich WD repeat containing 1 mm
MOUSE430_2:1455841_S_AT glutamate-rich WD repeat containing 1 mm
MU19KSUBA:TC14896_AT Glutamate-rich WD repeat containing 1 (Grwd1), mRNA mm
RAE230A:1374569_AT glutamate-rich WD repeat containing 1 (predicted) rn
RAT230_2:1374569_AT glutamate-rich WD repeat containing 1 (predicted) rn
YEAST_2:1769890_AT Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Sc
Protein Similarities
Method ID Description E-Value
blast AAF60780 Hypothetical protein Y54H5A.1 [Caenorhabditis elegans] ref|NP_498091.1| Y54H5A.1 [Caenorhabditis elegans] 0.0
blast CAE73768 Hypothetical protein CBG21312 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.1E-11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.8E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 7.7E-7
Sequence
Target Sequence 
>C. ELEGANS:184945_AT
gaagatcttgcatggtcaccaactgaaactggacttcttgcttcatgttccgcggatgga
agtatcaaactatgggatacacgttctgctccaaaagatgcctgcgtctgtacagtgcaa
aaagctcatgaatccgatgttaatgttatttcgtggaatcgtcacgaaaacctcattgtt
tctggtggtgatgacggagagctgaaaatttggtctttgaaaacaatccaattcggtcaa
ccagtggcactcttcaagtatcacaattcaccaatcacttcagtcgactggcatccacat
gaaacaacgacatttatggcttccggtgaagacgatcagacaacaatttgggatattgca
actgaagcagatggacaaacgaatatcgaaggagttccaccacagctgatgttcgtccat
atggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGATCTTGCATGGTCACCAACTG 458 337 895 Antisense
CTTGCTTCATGTTCCGCGGATGGAA 702 217 931 Antisense
ACTATGGGATACACGTTCTGCTCCA 7 101 963 Antisense
AAAGATGCCTGCGTCTGTACAGTGC 113 123 988 Antisense
GAATCGTCACGAAAACCTCATTGTT 487 329 1050 Antisense
AACCAGTGGCACTCTTCAAGTATCA 243 147 1133 Antisense
CAATTCACCAATCACTTCAGTCGAC 573 179 1158 Antisense
TTCAGTCGACTGGCATCCACATGAA 506 687 1173 Antisense
ACAACGACATTTATGGCTTCCGGTG 609 111 1198 Antisense
TCGAAGGAGTTCCACCACAGCTGAT 382 599 1280 Antisense
CACAGCTGATGTTCGTCCATATGGG 125 195 1295 Antisense
Sequence Source Affymetrix Proprietary Database