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[184943_s_at]

Description T02G5.11 /REP_DB=WormBase Gene ID /WP=CE04863 /TR=Q22105 /GB=AAA82402.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 10      6grp_cgc > 49      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 3.1
(-3)
A 		3.1
(-3)
A 		11.5
(-2.4)
A 		91.3
(3)
A 		88
B 8.5
(-3)
A 		28.8
(0)
A 		26.5
(-0.3)
A 		49.1
(3)
A 		41
C 25.4
(1.5)
A 		22.3
(0.8)
A 		7.3
(-3)
A 		31.3
(3)
A 		24
avr 12.3
(-3) 		18.1
(-2.2) 		15.1
(-2.6) 		57.2
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 937
1 2 3 4 5 6 range
A 8.6
(-3)
A 		2.7
(-3)
A 		26.8
(-2.8)
A 		11.5
(-2.9)
A 		327.1
(-0.6)
P 		806.1
(3)
P 		803
B 4.8
(-3)
A 		5.6
(-3)
A 		9.5
(-3)
A 		13.8
(-2.9)
A 		314.2
(-1)
P 		927.9
(3)
P 		923
C 4.8
(-3)
A 		6.7
(-3)
A 		3
(-3)
A 		42.2
(-2.7)
A 		354.2
(-0.8)
P 		939.7
(3)
P 		937
avr 6.1
(-3) 		5
(-3) 		13.1
(-2.9) 		22.5
(-2.9) 		331.8
(-0.8) 		891.2
(3) 		886
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 1.1
(-2.8)
A 		0.7
(-3)
A 		3
(-1.9)
A 		13.4
(3)
A 		13
B 2.7
(-3)
A 		7.1
(0.4)
A 		6.1
(-0.4)
A 		10.5
(3)
A 		8
C 8.4
(3)
A 		6.4
(0.9)
A 		2.6
(-3)
A 		5.3
(-0.2)
A 		6
avr 4.1
(-2.8) 		4.7
(-2.2) 		3.9
(-3) 		9.7
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 345
1 2 3 4 5 6 range
A 1.6
(-3)
A 		1
(-3)
A 		5.8
(-2.9)
A 		2.9
(-2.9)
A 		98.7
(0)
P 		196.3
(3)
P 		195
B 1.7
(-3)
A 		1.9
(-3)
A 		2.3
(-3)
A 		3.7
(-3)
A 		127.7
(-0.8)
P 		346
(3)
P 		344
C 1.8
(-3)
A 		2
(-3)
A 		0.8
(-3)
A 		8.5
(-2.9)
A 		114.6
(-0.8)
P 		314.6
(3)
P 		314
avr 1.7
(-3) 		1.6
(-3) 		3
(-3) 		5
(-2.9) 		113.7
(-0.6) 		285.6
(3) 		284
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184943_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4313
Sequence Type Exemplar sequence
Representative Public ID T02G5.11 NCBI
Target Description T02G5.11 /REP_DB=WormBase Gene ID /WP=CE04863 /TR=Q22105 /GB=AAA82402.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063051(11), NM_063059(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063051 NCBI Caenorhabditis elegans NanOS related family member (nos-2) (nos-2) mRNA, complete cds. 11/11 A 
NM_063059 NCBI Caenorhabditis elegans T02G5.11 (T02G5.11) mRNA, complete cds. 11/11 None
SNAP00000014287 ENSEMBL cdna:SNAP chromosome:CEL140:II:7080799:7081853:-1 11/11 None
SNAP00000033597 ENSEMBL cdna:SNAP chromosome:CEL140:II:7065604:7066575:1 11/11 None
GENEFINDER00000033603 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7065604:7066575:1 11/11 None
ZK1127.1 ENSEMBL cdna:known chromosome:CEL140:II:7065604:7066831:1 gene:ZK1127.1 11/11 A 
T02G5.11 ENSEMBL cdna:known chromosome:CEL140:II:7078509:7081853:-1 gene:T02G5.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000014300 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7081034-7081849(-) 87.94 100.0
Public Domain and Genome References
Gene Title

Gene Symbol nos-2
Ensembl ZK1127.1
T02G5.11
Entrez Gene 174158 Entrez gene
187994 Entrez gene
SwissProt Q22105 EMBL-EBI
Q23406 EMBL-EBI
WormBase CE37039 Wormbase
CE05121 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6445 regulation of translation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB93424 Nanos related protein 2 [Caenorhabditis elegans] ref|NP_495452.1| NanOS related family member (nos-2) [Caenorhabditis elegans] 1.0E-136
blast NP_495460 T02G5.11 [Caenorhabditis elegans] gb|AAA82402.2| Hypothetical protein T02G5.11 [Caenorhabditis elegans] 1.0E-107
blast AAB93424 Nanos related protein 2 [Caenorhabditis elegans] ref|NP_495452.1| NanOS related family member (nos-2) [Caenorhabditis elegans] 5.0E-99
blast NP_495460 T02G5.11 [Caenorhabditis elegans] gb|AAA82402.2| Hypothetical protein T02G5.11 [Caenorhabditis elegans] 1.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR008705 EMBL-EBI Nanos RNA-binding 4.3E-16
Pfam IPR008705 EMBL-EBI Nanos RNA-binding 2.5E-21
Pfam IPR008705 EMBL-EBI Nanos RNA-binding 2.5E-21
Sequence
Target Sequence 
>C. ELEGANS:184943_S_AT
acttccgacattcctgtcaacgaataattccggatttttcgattcagttgtcgcttcgga
tttcaatttgtacgggaagttcaatgattggttgaatgacagcaagcctcgcagtggatc
gtttgcgattgaatctgctgaagagctcgcgaggaatccaagagaaactcgctccgagcc
cgaagtgccatcattgttcaaaaggcgtgaatatggatgtggatactgtcgttccgttgg
atatatgcgatgggaaacgcatacacgtaagaaatgcgacaagctcagctcattggctcc
atgtaaaatttgtggagctcgcggtga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTCCGACATTCCTGTCAACGAAT 531 103 69 Antisense
ATAATTCCGGATTTTTCGATTCAGT 574 25 92 Antisense
GATTCAGTTGTCGCTTCGGATTTCA 123 429 109 Antisense
GGTTGAATGACAGCAAGCCTCGCAG 683 505 158 Antisense
GCAAGCCTCGCAGTGGATCGTTTGC 143 321 170 Antisense
GAATCTGCTGAAGAGCTCGCGAGGA 344 329 199 Antisense
TCCAAGAGAAACTCGCTCCGAGCCC 108 585 225 Antisense
GCCCGAAGTGCCATCATTGTTCAAA 87 287 246 Antisense
GTGGATACTGTCGTTCCGTTGGATA 74 491 287 Antisense
TGCGACAAGCTCAGCTCATTGGCTC 13 585 343 Antisense
GTAAAATTTGTGGAGCTCGCGGTGA 664 461 371 Antisense
Sequence Source Affymetrix Proprietary Database