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[184928_at]

Description C32B5.14 /REP_DB=WormBase Gene ID /WP=CE08522 /TR=P91112 /GB=AAB37969.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 68      4grp_total > 24      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 10.1
(-3)
A 		9.7
(-3)
A 		16.2
(-2.3)
A 		69.5
(3)
A 		60
B 11.9
(-3)
A 		67.2
(1)
A 		94.2
(3)
A 		26.4
(-1.9)
A 		82
C 10
(-3)
A 		11.5
(-2.9)
A 		33.3
(-1.2)
A 		88.9
(3)
A 		79
avr 10.7
(-3) 		29.5
(-0.8) 		47.9
(1.4) 		61.6
(3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 29.5
(-0.6)
A 		11.2
(-2.8)
A 		9.6
(-3)
A 		9.6
(-3)
A 		44.1
(1.2)
A 		59.3
(3)
A 		50
B 10.1
(-2.9)
A 		24.9
(-2)
A 		15.4
(-2.6)
A 		9.5
(-2.9)
A 		8.5
(-3)
A 		110.9
(3)
A 		102
C 25.2
(-2.1)
A 		25.4
(-2.1)
A 		7.9
(-2.9)
A 		9.6
(-2.8)
A 		6.1
(-3)
A 		135.6
(3)
A 		130
avr 21.6
(-2.2) 		20.5
(-2.3) 		11
(-2.9) 		9.6
(-3) 		19.6
(-2.3) 		101.9
(3) 		92
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 3.7
(-1.8)
A 		2.1
(-3)
A 		4.2
(-1.4)
A 		10.2
(3)
A 		8
B 3.8
(-3)
A 		16.6
(1.3)
A 		21.8
(3)
A 		5.6
(-2.4)
A 		18
C 3.3
(-3)
A 		3.3
(-3)
A 		11.6
(1.3)
A 		15
(3)
A 		12
avr 3.6
(-3) 		7.3
(-0.5) 		12.5
(3) 		10.3
(1.5) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 5.4
(-1.4)
A 		4
(-2.1)
A 		2.1
(-3)
A 		2.4
(-2.9)
A 		13.3
(2.5)
A 		14.4
(3)
A 		12
B 3.5
(-2.8)
A 		8.3
(-2.1)
A 		3.7
(-2.8)
A 		2.5
(-3)
A 		3.4
(-2.9)
A 		41.3
(3)
A 		39
C 9.3
(-2)
A 		7.8
(-2.2)
A 		2
(-3)
A 		1.9
(-3)
A 		2
(-3)
A 		45.4
(3)
A 		44
avr 6.1
(-2.3) 		6.7
(-2.2) 		2.6
(-2.9) 		2.3
(-3) 		6.2
(-2.3) 		33.7
(3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184928_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5689
Sequence Type Exemplar sequence
Representative Public ID C32B5.14 NCBI
Target Description C32B5.14 /REP_DB=WormBase Gene ID /WP=CE08522 /TR=P91112 /GB=AAB37969.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061467(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061467 NCBI Caenorhabditis elegans C32B5.14 (C32B5.14) mRNA, complete cds. 11/11 None
SNAP00000042178 ENSEMBL cdna:SNAP chromosome:CEL140:II:965676:966927:-1 11/11 None
GENEFINDER00000042194 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:965676:966965:-1 10/11 None
C32B5.14 ENSEMBL cdna:known chromosome:CEL140:II:965676:966965:-1 gene:C32B5.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:965674-966964(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32B5.14
Entrez Gene 183117 Entrez gene
SwissProt P91112 EMBL-EBI
WormBase CE08522 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_493868 C32B5.14 [Caenorhabditis elegans] gb|AAB37969.1| Hypothetical protein C32B5.14 [Caenorhabditis elegans] 0.0
blast NP_493865 C32B5.16 [Caenorhabditis elegans] gb|AAB37971.1| Hypothetical protein C32B5.16 [Caenorhabditis elegans] 1.0E-55
blast NP_493865 C32B5.16 [Caenorhabditis elegans] gb|AAB37971.1| Hypothetical protein C32B5.16 [Caenorhabditis elegans] 7.0E-54
blast NP_493865 C32B5.16 [Caenorhabditis elegans] gb|AAB37971.1| Hypothetical protein C32B5.16 [Caenorhabditis elegans] 3.0E-48
blast NP_493872 C32B5.4 [Caenorhabditis elegans] gb|AAB37960.1| Hypothetical protein C32B5.4 [Caenorhabditis elegans] 2.0E-39
Sequence
Target Sequence 
>C. ELEGANS:184928_AT
ggaagcgaaccaattctccattggatgtgacacagttcttcgggttccttcaacattgaa
gtttcgaattgaaaacctgtatactccgagttttaaaattgctaacacattggatgtagt
taatcaaatagttgacaactctagtctaccattaagctcactgaagtatagctttgaaaa
tcacatttatcatcatcctcattcacttgttagaaccgtaaagatgctgaaactcgaggt
cgaaatggtgcctgattatatctcaggaattgtaagcaacctacaaatggtggacgagaa
aagagctcacatagtatttctgggtgattgcacaagcagcaacttcttgaaaattctggc
tcactggattttggagtttcatagagacattggaacctatcacacttaccaattgagtga
agccgtcgttgatgaagtgatgatatttgttagaacaaattacggagtcatgattgaagc
tggactcccacaaaccaccgaccagataactctcaatataaacgacacttcgagccttgt
catc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAGCGAACCAATTCTCCATTGGA 674 529 594 Antisense
TGGATGTGACACAGTTCTTCGGGTT 53 551 615 Antisense
TTCTTCGGGTTCCTTCAACATTGAA 67 689 629 Antisense
AGTTGACAACTCTAGTCTACCATTA 538 63 723 Antisense
TATCATCATCCTCATTCACTTGTTA 684 659 781 Antisense
GCAACTTCTTGAAAATTCTGGCTCA 697 319 932 Antisense
GACATTGGAACCTATCACACTTACC 84 371 979 Antisense
GAGTGAAGCCGTCGTTGATGAAGTG 414 401 1008 Antisense
GATTGAAGCTGGACTCCCACAAACC 81 433 1065 Antisense
AACCACCGACCAGATAACTCTCAAT 592 147 1086 Antisense
AAACGACACTTCGAGCCTTGTCATC 596 125 1113 Antisense
Sequence Source Affymetrix Proprietary Database