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[184907_at]

Description C34D4.14 /REP_DB=WormBase Gene ID /WP=CE17508 /TR=Q18447 /GB=AAB00699.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 58      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 83      6grp_total > 48      4grp_total > 13      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 505
1 2 3 4 range
A 537.2
(0.3)
P 		429.2
(-1.5)
P 		341.6
(-3)
P 		695.9
(3)
P 		354
B 660.3
(1.1)
P 		355.4
(-2.5)
P 		317.1
(-3)
P 		821.9
(3)
P 		505
C 548.7
(-0.7)
P 		379
(-3)
P 		420.2
(-2.4)
P 		816.3
(3)
P 		437
avr 582.1
(0.2) 		387.9
(-2.6) 		359.6
(-3) 		778
(3) 		418

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 449
1 2 3 4 5 6 range
A 623.6
(0.8)
P 		603.4
(0.5)
P 		419.1
(-2.2)
P 		363
(-3)
P 		503.3
(-0.9)
P 		769.9
(3)
P 		407
B 673.7
(3)
P 		558.8
(0.8)
P 		490
(-0.6)
P 		502.2
(-0.3)
P 		366.5
(-3)
P 		632
(2.2)
P 		307
C 557.5
(2.1)
P 		473.6
(0.3)
P 		440.9
(-0.4)
P 		321.3
(-3)
P 		551.3
(2)
P 		597.9
(3)
P 		277
avr 618.3
(1.9) 		545.3
(0.3) 		450
(-1.8) 		395.5
(-3) 		473.7
(-1.3) 		666.6
(3) 		271
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 195.6
(3)
P 		93.2
(-2.8)
P 		89.3
(-3)
P 		101.9
(-2.3)
P 		106
B 210.6
(3)
P 		87.8
(-2.4)
P 		73.5
(-3)
P 		175.2
(1.5)
P 		137
C 181.7
(3)
P 		109
(-3)
P 		146.4
(0.1)
P 		137.6
(-0.6)
P 		73
avr 196
(3) 		96.7
(-3) 		103.1
(-2.6) 		138.2
(-0.5) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 115
(-1.8)
P 		214.6
(3)
P 		91.3
(-3)
P 		92.1
(-3)
P 		151.9
(-0.1)
P 		187.4
(1.7)
P 		123
B 234.4
(2.9)
P 		186
(0.4)
P 		119
(-3)
P 		133
(-2.3)
P 		148.9
(-1.5)
P 		235.7
(3)
P 		117
C 206.7
(3)
P 		144.5
(0.4)
P 		112.2
(-1)
P 		64.6
(-3)
P 		178.4
(1.8)
P 		200.1
(2.7)
P 		142
avr 185.4
(1.8) 		181.7
(1.6) 		107.5
(-2.4) 		96.6
(-3) 		159.7
(0.4) 		207.7
(3) 		111
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184907_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9116
Sequence Type Exemplar sequence
Representative Public ID C34D4.14 NCBI
Target Description C34D4.14 /REP_DB=WormBase Gene ID /WP=CE17508 /TR=Q18447 /GB=AAB00699.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068719(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068719 NCBI Caenorhabditis elegans C34D4.14 (C34D4.14) mRNA, complete cds. 11/11 None
SNAP00000003802 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7134840:7144547:-1 11/11 None
GENEFINDER00000003816 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7134840:7144547:-1 11/11 None
C34D4.14 ENSEMBL cdna:known chromosome:CEL140:IV:7134330:7144547:-1 gene:C34D4.14 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7134845-7144553(-) 99.69 100.0
Public Domain and Genome References
Gene Title
Ensembl C34D4.14
Entrez Gene 177486 Entrez gene
SwissProt Q18447 EMBL-EBI
WormBase CE17508 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605993_S_AT similar to HECT domain containing protein 1 cfa
CANINE:1597064_AT similar to HECT domain containing protein 1 cfa
CANINE_2:CFA.2009.1.S1_S_AT similar to HECT domain containing protein 1 cfa
CANINE_2:CFAAFFX.20016.1.S1_S_AT similar to HECT domain containing protein 1 cfa
DROSOPHILA_2:1634156_AT dm
DROSGENOME1:146135_AT dm
CHICKEN:GGA.3240.1.S1_AT HECT domain containing 1 gga
CHICKEN:GGAAFFX.23079.1.S1_S_AT HECT domain containing 1 gga
HG-U133B:224481_S_AT HECT domain containing 1 hs
HG-U133_PLUS_2:224481_S_AT HECT domain containing 1 hs
HU35KSUBA:RC_AA441821_AT HECT domain containing 1 hs
HU35KSUBA:W58540_AT HECT domain containing 1 hs
U133_X3P:G13623274_3P_S_AT HECT domain containing 1 hs
U133_X3P:HS2.421481.1.S1_3P_X_AT HECT domain containing 1 hs
U133_X3P:HS.322848.0.A1_3P_AT HECT domain containing 1 hs
U133_X3P:HS.314346.0.S1_3P_AT HECT domain containing 1 hs
U133_X3P:HS.190223.0.S1_3P_AT HECT domain containing 1 hs
HG-U133_PLUS_2:1557100_S_AT HECT domain containing 1 hs
HG-U133_PLUS_2:241683_AT HECT domain containing 1 hs
HG-U133B:241683_AT HECT domain containing 1 hs
HG-U133_PLUS_2:241955_AT HECT domain containing 1 hs
HG-U133B:241955_AT HECT domain containing 1 hs
HG-U133_PLUS_2:242349_AT HECT domain containing 1 hs
HG-U133B:242349_AT HECT domain containing 1 hs
HG-U95B:45304_AT HECT domain containing 1 hs
HG-U95E:68210_AT HECT domain containing 1 hs
HG-U95D:72016_AT HECT domain containing 1 hs
HG-U95D:85047_AT HECT domain containing 1 hs
U133_X3P:HS2.407239.1.S1_3P_AT HECT domain containing 1 hs
HG-U133_PLUS_2:1570251_AT HECT domain containing 1 hs
HG-U95E:78021_AT HECT domain containing 1 hs
HG-U95D:81798_AT HECT domain containing 1 hs
HG-U95E:86985_I_AT HECT domain containing 1 hs
HG-U95E:86988_R_AT HECT domain containing 1 hs
MG-U74BV2:108507_AT HECT domain containing 1 mm
MG-U74BV2:116116_AT HECT domain containing 1 mm
MOE430A:1424141_AT HECT domain containing 1 mm
MOUSE430A_2:1424141_AT HECT domain containing 1 mm
MOUSE430_2:1424141_AT HECT domain containing 1 mm
MOE430B:1438039_AT HECT domain containing 1 mm
MOUSE430_2:1438039_AT HECT domain containing 1 mm
MU19KSUBB:TC31559_AT HECT domain containing 1, mRNA (cDNA clone IMAGE:3708271) mm
MU19KSUBB:TC31559_G_AT HECT domain containing 1, mRNA (cDNA clone IMAGE:3708271) mm
MU19KSUBC:TC36278_AT HECT domain containing 1, mRNA (cDNA clone IMAGE:3708271) mm
RG-U34B:RC_AI059295_AT similar to HECT domain containing 1 rn
RG-U34C:RC_AI228159_AT similar to HECT domain containing 1 rn
RG-U34C:RC_AI178224_AT similar to HECT domain containing 1 rn
RG-U34C:RC_AI175474_AT similar to HECT domain containing 1 rn
RAE230A:1372394_AT similar to HECT domain containing 1 rn
RAT230_2:1372394_AT similar to HECT domain containing 1 rn
RAE230B:1392259_AT similar to HECT domain containing 1 rn
RAT230_2:1392259_AT similar to HECT domain containing 1 rn
RAE230B:1383780_AT similar to HECT domain containing 1 rn
RAT230_2:1383780_AT similar to HECT domain containing 1 rn
RAE230B:1383615_A_AT similar to HECT domain containing 1 rn
RAT230_2:1383615_A_AT similar to HECT domain containing 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00699 Hypothetical protein C34D4.14 [Caenorhabditis elegans] ref|NP_501120.1| C34D4.14 [Caenorhabditis elegans] 0.0
blast CAE61812 Hypothetical protein CBG05779 [Caenorhabditis briggsae] 0.0
blast EAL41378 ENSANGP00000025754 [Anopheles gambiae str. PEST] ref|XP_559736.1| ENSANGP00000025754 [Anopheles gambiae str. PEST] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002110 EMBL-EBI Ankyrin 0.027
Pfam IPR002110 EMBL-EBI Ankyrin 44.0
Pfam IPR002110 EMBL-EBI Ankyrin 0.0024
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 9.2E-39
Pfam IPR010606 EMBL-EBI Mib_herc2 2.7E-32
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 9.2E-39
Pfam IPR010606 EMBL-EBI Mib_herc2 2.7E-32
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 9.2E-39
Sequence
Target Sequence 
>C. ELEGANS:184907_AT
gatcttgcacttaatttcactgtcaatccgccatcaaaagtgttccaatatgccgaaatg
gagttggttgacggtggatccgacatcgatgttacaatcgacaacgtggagcaatatgtg
gagaaatgtgaagaattctatttgaacacaggaatagcctaccagatgcgtgctttccgt
gatggattcgatcgcgtttttccacttagaactctcagagcatactctccagaagaagtt
cagcgtcttttgtccggtgaacagtgcccagaatggtctcgggacgatattctaaattat
acggagccaaaacttggatacactcgagaatctcctggtttcttgagatttgtcgatgta
atggaagcattgacagctcaagaacgaaagaatttccttcaattcgccacaggatgttcg
agcttgccaccaggtggtcttgctaatcttcatccaaggttgacaattgtgcgaaaagtg
gaaagtggagacggcagctatccatcggttaatacatgtgtgcattatctcaaactcccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTTGCACTTAATTTCACTGTCA 9 421 7684 Antisense
GTCAATCCGCCATCAAAAGTGTTCC 332 463 7705 Antisense
ATAGCCTACCAGATGCGTGCTTTCC 343 23 7837 Antisense
GATCGCGTTTTTCCACTTAGAACTC 192 417 7873 Antisense
GAACTCTCAGAGCATACTCTCCAGA 625 349 7892 Antisense
GTCTTTTGTCCGGTGAACAGTGCCC 184 469 7928 Antisense
ACTCGAGAATCTCCTGGTTTCTTGA 374 99 8005 Antisense
TCGCCACAGGATGTTCGAGCTTGCC 400 599 8087 Antisense
CAGGTGGTCTTGCTAATCTTCATCC 242 209 8114 Antisense
GGCAGCTATCCATCGGTTAATACAT 136 537 8176 Antisense
ATGTGTGCATTATCTCAAACTCCCG 660 49 8199 Antisense
Sequence Source Affymetrix Proprietary Database