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[184906_at]

Description T20D4.10 /REP_DB=WormBase Gene ID /WP=CE13798 /TR=P91465 /GB=AAB37587.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 34      4grp_total > 04      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 25.4
(-0)
A 		9
(-3)
A 		36.6
(2)
A 		42.3
(3)
A 		33
B 45.9
(3)
A 		10.4
(-2.8)
A 		9.1
(-3)
A 		38.1
(1.7)
A 		37
C 26
(-1.7)
A 		44.3
(-0.2)
A 		9.3
(-3)
A 		84.6
(3)
A 		75
avr 32.4
(-0.7) 		21.2
(-2.5) 		18.3
(-3) 		55
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 52.4
(3)
A 		7.8
(-3)
A 		41.7
(1.6)
A 		7.9
(-3)
A 		8
(-3)
A 		12
(-2.4)
A 		45
B 19.7
(-1)
A 		45.7
(2.5)
A 		49.6
(3)
A 		9.6
(-2.4)
A 		5.1
(-3)
A 		16
(-1.5)
A 		45
C 4.7
(-3)
A 		41.1
(3)
A 		7.3
(-2.5)
A 		7.8
(-2.5)
A 		4.5
(-3)
A 		5.9
(-2.8)
A 		37
avr 25.6
(1.4) 		31.5
(2.7) 		32.9
(3) 		8.4
(-2.4) 		5.9
(-3) 		11.3
(-1.8) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 9.2
(2.7)
A 		2
(-3)
A 		9.6
(3)
A 		6.2
(0.3)
A 		8
B 14.6
(3)
A 		2.6
(-2.8)
A 		2.1
(-3)
A 		8.1
(-0.1)
A 		13
C 8.6
(-0.1)
A 		12.7
(2.1)
A 		3.2
(-3)
A 		14.3
(3)
A 		11
avr 10.8
(3) 		5.8
(-2.2) 		5
(-3) 		9.5
(1.7) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 9.7
(3)
A 		2.8
(-2.4)
A 		9.1
(2.5)
A 		2
(-3)
A 		2.4
(-2.7)
A 		2.9
(-2.3)
A 		8
B 6.9
(-0.8)
A 		15.2
(3)
A 		12
(1.5)
A 		2.6
(-2.8)
A 		2.1
(-3)
A 		6
(-1.2)
A 		13
C 1.7
(-2.9)
A 		12.5
(3)
A 		1.8
(-2.8)
A 		1.6
(-2.9)
A 		1.5
(-3)
A 		2
(-2.7)
A 		11
avr 6.1
(0) 		10.2
(3) 		7.6
(1.1) 		2.1
(-2.9) 		2
(-3) 		3.6
(-1.8) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184906_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14924
Sequence Type Exemplar sequence
Representative Public ID T20D4.10 NCBI
Target Description T20D4.10 /REP_DB=WormBase Gene ID /WP=CE13798 /TR=P91465 /GB=AAB37587.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071550(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071550 NCBI Caenorhabditis elegans T20D4.10 (T20D4.10) mRNA, complete cds. 11/11 None
SNAP00000008847 ENSEMBL cdna:SNAP chromosome:CEL140:V:3401354:3405021:1 10/11 None
GENEFINDER00000008863 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3401471:3405021:1 10/11 None
T20D4.10 ENSEMBL cdna:known chromosome:CEL140:V:3400081:3402182:1 gene:T20D4.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3400080-3402182(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D4.10
Entrez Gene 188646 Entrez gene
SwissProt P91465 EMBL-EBI
WormBase CE13798 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37587 Hypothetical protein T20D4.10 [Caenorhabditis elegans] ref|NP_503951.1| T20D4.10 [Caenorhabditis elegans] 0.0
blast AAF59425 Hypothetical protein Y19D10B.6 [Caenorhabditis elegans] ref|NP_503636.1| Y19D10B.6 [Caenorhabditis elegans] 0.0
blast AAB37588 Hypothetical protein T20D4.9 [Caenorhabditis elegans] ref|NP_503952.1| T20D4.9 [Caenorhabditis elegans] 0.0
blast AAB37589 Hypothetical protein T20D4.8 [Caenorhabditis elegans] ref|NP_503953.1| T20D4.8 [Caenorhabditis elegans] 0.0
blast AAC04403 Hypothetical protein C17B7.9 [Caenorhabditis elegans] ref|NP_503923.1| C17B7.9 [Caenorhabditis elegans] 4.0E-78
Protein Domains
Database ID Description E-Value
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 3.0E-83
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 5.9E-38
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 3.1E-88
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 9.3E-36
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 3.1E-88
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 9.3E-36
Sequence
Target Sequence 
>C. ELEGANS:184906_AT
ttgtcgacactacttctcattggaataatctgtttacagcgattaggagaattcagtgca
agattgttctttgtcgatttgaagcagaagagcctggagaaatttacaaaatcgtgcact
tccatatttacaaaatcgtgcacttccacacatgtcttgaccttgttgagtgtgaaaaag
agcaagacaactccacaggaaaacaagttagggagtcctgtgagatcatgaacttcttat
ccacagacttcaatccctgtcttgcaaagttgggaaacttgaaaccagttcccgagtgtt
tcaaagagtggaaaccattttatgatttggtattaagtggagaggacgaaaccacagacg
aaatgggggaactgtgcaaaaacatggttggaaaagaaaattgcgtgaagaagttggtca
gcgaaacgtgtggagagccggaatcccagcagttgtttaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTCGACACTACTTCTCATTGGAA 496 705 581 Antisense
AATCGTGCACTTCCATATTTACAAA 343 169 690 Antisense
TATTTACAAAATCGTGCACTTCCAC 84 661 705 Antisense
ACTTCCACACATGTCTTGACCTTGT 58 105 722 Antisense
ATGTCTTGACCTTGTTGAGTGTGAA 9 47 732 Antisense
GGAGTCCTGTGAGATCATGAACTTC 459 517 792 Antisense
GAACTTCTTATCCACAGACTTCAAT 205 351 810 Antisense
GACTTCAATCCCTGTCTTGCAAAGT 643 371 826 Antisense
ACCAGTTCCCGAGTGTTTCAAAGAG 602 85 864 Antisense
GTCAGCGAAACGTGTGGAGAGCCGG 388 455 997 Antisense
CCGGAATCCCAGCAGTTGTTTAATA 501 263 1018 Antisense
Sequence Source Affymetrix Proprietary Database