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[184899_s_at]

Description Y54H5A.4 /REP_DB=WormBase Gene ID /WP=CE24460 /TR=Q9N392 /GB=AAF60782.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 54      4grp_high_8_8 > 16      6grp_total > 54      4grp_total > 39      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 152.8
(3)
P 		82
(-3)
A 		91.1
(-2.2)
P 		110.5
(-0.6)
A 		71
B 175
(3)
P 		98.1
(-3)
P 		115.3
(-1.7)
P 		108.6
(-2.2)
P 		77
C 167.5
(3)
P 		104.6
(-3)
P 		131.8
(-0.4)
P 		148.5
(1.2)
P 		63
avr 165.1
(3) 		94.9
(-3) 		112.7
(-1.5) 		122.5
(-0.6) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 114.1
(-1.9)
P 		117.2
(-1.7)
P 		201.4
(3)
P 		124.9
(-1.3)
P 		94.3
(-3)
P 		96.7
(-2.9)
M 		107
B 166.8
(2.2)
P 		157.4
(0)
P 		170.1
(3)
P 		145.8
(-2.7)
P 		144.6
(-3)
P 		162.3
(1.2)
P 		26
C 122.5
(-2.6)
P 		166.3
(3)
P 		141.2
(-0.2)
P 		140.4
(-0.3)
A 		119.2
(-3)
P 		139.2
(-0.5)
P 		47
avr 134.5
(-1.2) 		147
(0.2) 		170.9
(3) 		137
(-0.9) 		119.4
(-3) 		132.7
(-1.5) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 55.6
(3)
P 		17.8
(-2.8)
A 		23.8
(-1.8)
P 		16.2
(-3)
A 		39
B 55.8
(3)
P 		24.2
(-2.8)
P 		26.7
(-2.3)
P 		23.1
(-3)
P 		33
C 55.4
(3)
P 		30.1
(-2)
P 		45.9
(1.1)
P 		25
(-3)
P 		30
avr 55.6
(3) 		24
(-2.5) 		32.1
(-1.1) 		21.4
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 21
(-3)
P 		41.7
(2.4)
P 		43.9
(3)
P 		31.7
(-0.2)
P 		28.5
(-1)
P 		23.5
(-2.3)
M 		23
B 58
(2.3)
P 		52.4
(0.8)
P 		41.3
(-2.3)
P 		38.6
(-3)
P 		58.8
(2.5)
P 		60.5
(3)
P 		22
C 45.4
(1.6)
P 		50.8
(3)
P 		35.9
(-1)
P 		28.2
(-3)
A 		38.6
(-0.2)
P 		46.6
(1.9)
P 		23
avr 41.5
(0.4) 		48.3
(3) 		40.4
(-0.1) 		32.8
(-3) 		42
(0.6) 		43.5
(1.1) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184899_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7564
Sequence Type Exemplar sequence
Representative Public ID Y54H5A.4 NCBI
Target Description Y54H5A.4 /REP_DB=WormBase Gene ID /WP=CE24460 /TR=Q9N392 /GB=AAF60782.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065691(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065691 NCBI Caenorhabditis elegans Y54H5A.4 (Y54H5A.4) mRNA, complete cds. 11/11 None
SNAP00000038485 ENSEMBL cdna:SNAP chromosome:CEL140:III:5155489:5158724:-1 11/11 None
GENEFINDER00000038490 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5155489:5159288:-1 11/11 None
Y54H5A.4.1 ENSEMBL cdna:known chromosome:CEL140:III:5155461:5158730:-1 gene:Y54H5A.4 11/11 None
Y54H5A.4.2 ENSEMBL cdna:known chromosome:CEL140:III:5155461:5158732:-1 gene:Y54H5A.4 11/11 None
Y54H5A.4.3 ENSEMBL cdna:known chromosome:CEL140:III:5155489:5158731:-1 gene:Y54H5A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5155487-5159227(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54H5A.4
Entrez Gene 175702 Entrez gene
SwissProt Q9N392 EMBL-EBI
WormBase CE29883 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60782 Hypothetical protein Y54H5A.4 [Caenorhabditis elegans] ref|NP_498092.4| Y54H5A.4 [Caenorhabditis elegans] 0.0
blast CAE73774 Hypothetical protein CBG21318 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004108 EMBL-EBI Hydrogenase large subunit, C-terminal 2.1E-32
Pfam IPR004108 EMBL-EBI Hydrogenase large subunit, C-terminal 8.9E-18
Pfam IPR004108 EMBL-EBI Hydrogenase large subunit, C-terminal 2.1E-32
Pfam IPR004108 EMBL-EBI Hydrogenase large subunit, C-terminal 8.9E-18
Sequence
Target Sequence 
>C. ELEGANS:184899_S_AT
caatggttatcggctctttctaaaggatctgttattggtgacgatggtggtgcttctgga
ggatatgctgatcgaattgttcgagatttcgtgctggaaaatggaggaggaattgtaaaa
acatcaaaactaaacaagaacatgttctccacgactgtagaatccgaagctggagaaatt
cttttaagagttgccaaggtttacgggtttcgaaatgtgcagaatctggttcgaaaaatg
aagacgaaaaaggagaagacagattacgtggaaattatggcttgtccgggtggatgtgca
aatggtggaggtcagattcggtacgagacaatggatgagagagaagagaaattgattaaa
gtcgaggcattatatgaagatttacccagacaagatgatgaagaaacctggataaaagtt
cgagaggaatgggagaaacttgataaaaactaccgtaatcttctgtttacggactatagg
c

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATGGTTATCGGCTCTTTCTAAAG 649 181 1204 Antisense
ATTGGTGACGATGGTGGTGCTTCTG 512 3 1237 Antisense
GGTGCTTCTGGAGGATATGCTGATC 99 501 1252 Antisense
GAATTGTTCGAGATTTCGTGCTGGA 383 323 1277 Antisense
AGAACATGTTCTCCACGACTGTAGA 555 73 1340 Antisense
CACGACTGTAGAATCCGAAGCTGGA 167 199 1353 Antisense
GGTTTACGGGTTTCGAAATGTGCAG 677 443 1401 Antisense
GAAATTATGGCTTGTCCGGGTGGAT 156 361 1474 Antisense
GTCCGGGTGGATGTGCAAATGGTGG 75 475 1487 Antisense
GGTGGAGGTCAGATTCGGTACGAGA 467 493 1507 Antisense
AATCTTCTGTTTACGGACTATAGGC 494 141 1660 Antisense
Sequence Source Affymetrix Proprietary Database