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[184893_at] 7TM chemoreceptor, srj family

Description T28A11.9 /REP_DB=WormBase Gene ID /WP=CE14297 /TR=P91507 /GB=AAC48121.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 45      4grp_total > 19      6grp_cgc > 45      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 28.5
(-2.7)
A 		28.4
(-2.8)
A 		24.4
(-3)
A 		121.4
(3)
A 		97
B 35.6
(0.4)
A 		52
(2.3)
A 		7.4
(-3)
A 		57.6
(3)
A 		50
C 40.7
(-1.2)
A 		55.1
(0.1)
A 		21.8
(-3)
A 		86.1
(3)
P 		64
avr 34.9
(-1.6) 		45.2
(-0.7) 		17.9
(-3) 		88.4
(3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 29.6
(1)
A 		20.9
(-1.3)
A 		36.3
(2.8)
A 		33.3
(2)
A 		14.6
(-3)
A 		37.1
(3)
A 		23
B 45.3
(1.5)
A 		36.4
(0.4)
A 		58.1
(3)
A 		16.4
(-1.9)
A 		7.4
(-3)
A 		26.5
(-0.7)
A 		51
C 16.4
(-0.9)
A 		41.9
(3)
A 		18.3
(-0.6)
A 		2.6
(-3)
A 		4.3
(-2.7)
A 		21.2
(-0.2)
A 		39
avr 30.4
(1.5) 		33.1
(2.1) 		37.6
(3) 		17.4
(-1.2) 		8.8
(-3) 		28.3
(1.1) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 10.4
(-0.8)
A 		6.2
(-3)
A 		6.4
(-2.9)
A 		17.8
(3)
A 		12
B 11.4
(2.2)
A 		12.8
(3)
A 		1.7
(-3)
A 		12.3
(2.7)
A 		11
C 13.5
(1.3)
A 		15.8
(3)
A 		7.6
(-3)
A 		14.5
(2)
P 		8
avr 11.8
(1.1) 		11.6
(1) 		5.2
(-3) 		14.9
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 5.5
(-1.6)
A 		7.4
(0.9)
A 		7.9
(1.6)
A 		8.4
(2.2)
A 		4.4
(-3)
A 		9
(3)
A 		5
B 15.7
(3)
A 		12.1
(1.3)
A 		14.1
(2.2)
A 		4.3
(-2.4)
A 		3
(-3)
A 		9.9
(0.3)
A 		13
C 6.1
(-0.3)
A 		12.8
(3)
A 		4.7
(-1)
A 		0.5
(-3)
A 		1.4
(-2.6)
A 		7.1
(0.2)
A 		12
avr 9.1
(1.7) 		10.8
(3) 		8.9
(1.6) 		4.4
(-1.9) 		2.9
(-3) 		8.7
(1.4) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184893_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14984
Sequence Type Exemplar sequence
Representative Public ID T28A11.9 NCBI
Target Description T28A11.9 /REP_DB=WormBase Gene ID /WP=CE14297 /TR=P91507 /GB=AAC48121.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071490(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071490 NCBI Caenorhabditis elegans Serpentine Receptor, class J family member (srj-8) (srj-8) mRNA, complete cds. 11/11 None
SNAP00000028202 ENSEMBL cdna:SNAP chromosome:CEL140:V:3247753:3248967:1 11/11 None
GENEFINDER00000028224 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3245150:3248967:1 11/11 None
T28A11.9 ENSEMBL cdna:known chromosome:CEL140:V:3247642:3248967:1 gene:T28A11.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3247641-3248967(+) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srj family
Gene Symbol srj-8
Ensembl T28A11.9
Entrez Gene 189015 Entrez gene
SwissProt P91507 EMBL-EBI
WormBase CE14297 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503891 Serpentine Receptor, class J family member (srj-8) [Caenorhabditis elegans] gb|AAC48121.1| Serpentine receptor, class j protein 8 [Caenorhabditis elegans] 0.0
blast NP_503891 Serpentine Receptor, class J family member (srj-8) [Caenorhabditis elegans] gb|AAC48121.1| Serpentine receptor, class j protein 8 [Caenorhabditis elegans] 1.0E-166
blast NP_503890 Serpentine Receptor, class J family member (srj-7) [Caenorhabditis elegans] gb|AAC48131.1| Serpentine receptor, class j protein 7 [Caenorhabditis elegans] 1.0E-139
blast AAD14729 Serpentine receptor, class j protein 9 [Caenorhabditis elegans] ref|NP_503963.1| Serpentine Receptor, class J family member (srj-9) [Caenorhabditis elegans] 1.0E-134
blast NP_503890 Serpentine Receptor, class J family member (srj-7) [Caenorhabditis elegans] gb|AAC48131.1| Serpentine receptor, class j protein 7 [Caenorhabditis elegans] 1.0E-120
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 7.7E-9
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 3.7E-8
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 7.7E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503891.1 7 10-32,44-66,92-114,127-149,199-221,247-269,284-306
T28A11.9 7 10-32,44-66,92-114,127-149,199-221,247-269,284-306
SNAP00000028202 6 7-29,55-77,90-112,172-194,220-242,257-279
GENEFINDER00000028224 11 5-27,34-56,107-129,155-177,192-214,267-289,349-371,384-406,456-478,504-526,541-563
Sequence
Target Sequence 
>C. ELEGANS:184893_AT
catggtgcatcgtggtctgtgatctgccagcaatgccttggcggtactgatgagatccga
gagctgatccgggatgagtttatggcagaataccacgcagataccagggatgtaccaatg
ctggctgcactttattggaacgcaagtgacgctattcgtttccgtagttggctcggcatc
gtcttactgactgtgatctcattttatgcgatgacaatttattttgtgttgggctacaag
attatgagaaaaattcggtcaatgcaagcgaacagcagtatgagcaagaacagcattcgg
ctgcagaaaaaactatttttgacgctaattattcagacctgcataccaatattcgccagc
tttctacctacagtactctcgtggtatgctccaattttcggaattgatttgtcttggtgg
aacaccaacgtggctaccgtagccctttctgctttcccattcatcgatccgttggctgtg
atctacttggtgccaagctatcggaacgcgattttgagaaaaagagacccgacggtggct
agcagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATGGTGCATCGTGGTCTGTGATCT 466 209 439 Antisense
GATCTGCCAGCAATGCCTTGGCGGT 683 417 459 Antisense
GATGTACCAATGCTGGCTGCACTTT 593 409 547 Antisense
CGCAAGTGACGCTATTCGTTTCCGT 144 255 579 Antisense
TCGTTTCCGTAGTTGGCTCGGCATC 22 601 594 Antisense
CTCGGCATCGTCTTACTGACTGTGA 134 233 610 Antisense
GACCTGCATACCAATATTCGCCAGC 656 381 774 Antisense
TACAGTACTCTCGTGGTATGCTCCA 102 645 807 Antisense
CATTCATCGATCCGTTGGCTGTGAT 641 215 896 Antisense
GGCTGTGATCTACTTGGTGCCAAGC 310 541 912 Antisense
AGAGACCCGACGGTGGCTAGCAGTT 615 67 961 Antisense
Sequence Source Affymetrix Proprietary Database