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[184873_at]

Description F46F5.14 /REP_DB=WormBase Gene ID /WP=CE19430 /TR=Q9TXX5 /GB=AAC78192.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 05      4grp_total > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 4.1
(-3)
A 		5
(-2.9)
A 		5.9
(-2.9)
A 		83.6
(3)
A 		80
B 4.7
(3)
A 		3.9
(0.5)
A 		2.8
(-3)
A 		4.3
(1.7)
A 		2
C 1.8
(-3)
A 		2.2
(-2.9)
A 		4.4
(-2.4)
A 		30.1
(3)
A 		28
avr 3.5
(-3) 		3.7
(-3) 		4.4
(-2.8) 		39.3
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 8.4
(-0.3)
A 		16.8
(3)
A 		5.5
(-1.5)
A 		6.6
(-1.1)
A 		1.7
(-3)
A 		6.4
(-1.1)
A 		15
B 31.6
(3)
A 		2.4
(-3)
A 		3.7
(-2.7)
A 		7.4
(-2)
A 		16.6
(-0.1)
A 		3
(-2.9)
A 		29
C 5.9
(-2.1)
A 		20.8
(3)
A 		6.3
(-2)
A 		4
(-2.8)
A 		4.3
(-2.7)
A 		3.4
(-3)
A 		17
avr 15.3
(3) 		13.3
(1.9) 		5.2
(-2.5) 		6
(-2.1) 		7.5
(-1.3) 		4.3
(-3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 1.5
(-2.8)
A 		1.1
(-3)
A 		1.5
(-2.8)
A 		12.2
(3)
A 		11
B 1.5
(3)
A 		1
(-0.8)
A 		0.7
(-3)
A 		0.9
(-1.5)
A 		1
C 0.6
(-3)
A 		0.6
(-3)
A 		1.5
(-1.8)
A 		5.1
(3)
A 		5
avr 1.2
(-2.7) 		0.9
(-3) 		1.2
(-2.7) 		6.1
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 1.6
(-1.8)
A 		6
(3)
A 		1.2
(-2.2)
A 		1.7
(-1.7)
A 		0.5
(-3)
A 		1.6
(-1.8)
A 		6
B 11
(3)
A 		0.8
(-3)
A 		0.9
(-2.9)
A 		2
(-2.3)
A 		6.7
(0.5)
A 		1.1
(-2.8)
A 		10
C 2.2
(-1.5)
A 		6.3
(3)
A 		1.6
(-2.1)
A 		0.8
(-3)
A 		1.4
(-2.3)
A 		1.1
(-2.7)
A 		6
avr 4.9
(3) 		4.4
(2.2) 		1.2
(-3) 		1.5
(-2.5) 		2.9
(-0.2) 		1.3
(-2.8) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184873_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5451
Sequence Type Exemplar sequence
Representative Public ID F46F5.14 NCBI
Target Description F46F5.14 /REP_DB=WormBase Gene ID /WP=CE19430 /TR=Q9TXX5 /GB=AAC78192.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061412(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061412 NCBI Caenorhabditis elegans F46F5.14 (F46F5.14) mRNA, complete cds. 10/11 None
GENEFINDER00000029529 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:800089:801460:1 11/11 None
F46F5.14 ENSEMBL cdna:known chromosome:CEL140:II:800089:801460:1 gene:F46F5.14 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000029516 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:800088-801460(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F46F5.14
Entrez Gene 185867 Entrez gene
SwissProt Q9TXX5 EMBL-EBI
WormBase CE35936 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78192 Hypothetical protein F46F5.14 [Caenorhabditis elegans] ref|NP_493813.2| F46F5.14 [Caenorhabditis elegans] 0.0
blast AAC19260 Hypothetical protein ZK1055.2 [Caenorhabditis elegans] ref|NP_504676.1| ZK1055.2 [Caenorhabditis elegans] 1.0E-124
blast AAC19260 Hypothetical protein ZK1055.2 [Caenorhabditis elegans] ref|NP_504676.1| ZK1055.2 [Caenorhabditis elegans] 1.0E-123
blast CAB04382 Hypothetical protein F41D3.11 [Caenorhabditis elegans] ref|NP_493100.1| F41D3.11 [Caenorhabditis elegans] 1.0E-117
blast CAB04382 Hypothetical protein F41D3.11 [Caenorhabditis elegans] ref|NP_493100.1| F41D3.11 [Caenorhabditis elegans] 1.0E-116
Protein Domains
Database ID Description E-Value
Pfam IPR004988 EMBL-EBI Protein of unknown function DUF273 1.0E-126
Pfam IPR004988 EMBL-EBI Protein of unknown function DUF273 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493813.2 1 12-31
F46F5.14 1 12-31
GENEFINDER00000029529 1 12-31
Sequence
Target Sequence 
>C. ELEGANS:184873_AT
tgcagctggctattaccttgtaagaaatacgacgttcgcagttgacttacttaatgaatt
tgctgattatgaatacaaactgccgaatagctttcacgggactgacaatggagcgcttca
tattcttcttgctgaaaaactattcccatacgcctcgatcgagactgagatgtgcagaag
tgcctataacaaatccaaaaattttaatgatttgttcacatatgaagcatgtatcaaagc
aatgatgggtgtcggaacggattttggaaaagttcgaattctgaaaaaagtcggaaccgg
ttgggctagagatggatggctgacttcaatgatgtggcatcctgaaatagattttatgat
tcacggttggaaatctaatcagttgaaaccaacaccaaacactcctctgaaaccaaaaga
aatgggaacaagtcaatggtacaacccgttgaacgggacgattgatcttgcaaagtgtca
ccctggtaactctacatggtcgttcgatcggcgccttcttggggacaaagagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCAGCTGGCTATTACCTTGTAAGA 141 577 459 Antisense
ATACGACGTTCGCAGTTGACTTACT 134 9 485 Antisense
AACTGCCGAATAGCTTTCACGGGAC 174 141 536 Antisense
CACGGGACTGACAATGGAGCGCTTC 599 199 553 Antisense
AATGGAGCGCTTCATATTCTTCTTG 178 163 565 Antisense
TGAAAAACTATTCCCATACGCCTCG 433 569 591 Antisense
AGTCGGAACCGGTTGGGCTAGAGAT 387 59 747 Antisense
TTGATCTTGCAAAGTGTCACCCTGG 198 707 920 Antisense
GTCACCCTGGTAACTCTACATGGTC 605 465 935 Antisense
ACTCTACATGGTCGTTCGATCGGCG 669 97 947 Antisense
CGGCGCCTTCTTGGGGACAAAGAGG 490 241 967 Antisense
Sequence Source Affymetrix Proprietary Database