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[184868_at]

Description F12A10.1 /REP_DB=WormBase Gene ID /WP=CE01904 /TR=Q09943 /GB=AAA68294.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 77      6grp_highest_nonorm > 94      6grp_total > 67      4grp_total > 02      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1135
1 2 3 4 range
A 105.5
(-2.9)
P 		123
(-2.8)
M 		83.2
(-3)
A 		1209.4
(3)
P 		1126
B 96.8
(-2.9)
P 		77.5
(-3)
A 		82.3
(-3)
P 		852.4
(3)
P 		775
C 102.5
(-2.9)
P 		94.7
(-2.9)
A 		74.8
(-3)
A 		1188.3
(3)
P 		1114
avr 101.6
(-2.9) 		98.4
(-2.9) 		80.1
(-3) 		1083.4
(3) 		1003

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1977
1 2 3 4 5 6 range
A 303.3
(-2.2)
P 		159.8
(-3)
P 		368.9
(-1.9)
P 		273
(-2.4)
P 		318.1
(-2.2)
P 		1278.6
(3)
P 		1119
B 355.5
(-2.6)
P 		241.3
(-3)
P 		302.2
(-2.8)
P 		249.3
(-3)
P 		472.2
(-2.2)
P 		1987.5
(3)
P 		1746
C 278.6
(-2.8)
P 		223.8
(-3)
P 		233.9
(-3)
P 		332.7
(-2.7)
P 		370.4
(-2.5)
P 		2137.2
(3)
P 		1913
avr 312.5
(-2.6) 		208.3
(-3) 		301.7
(-2.6) 		285
(-2.7) 		386.9
(-2.3) 		1801.1
(3) 		1593
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 38.4
(-2.4)
P 		26.7
(-2.8)
M 		21.8
(-3)
A 		177
(3)
P 		155
B 30.9
(-2.6)
P 		19.1
(-3)
A 		19.1
(-3)
P 		181.6
(3)
P 		163
C 33.9
(-2.7)
P 		27.2
(-3)
A 		26.1
(-3)
A 		200.4
(3)
P 		174
avr 34.4
(-2.6) 		24.3
(-2.9) 		22.3
(-3) 		186.3
(3) 		164

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 685
1 2 3 4 5 6 range
A 55.9
(-3)
P 		56.8
(-3)
P 		80.3
(-2.4)
P 		69.2
(-2.7)
P 		96
(-2.1)
P 		311.3
(3)
P 		255
B 123.7
(-2.5)
P 		80.3
(-2.9)
P 		73.4
(-2.9)
P 		66
(-3)
P 		191.9
(-1.9)
P 		741.1
(3)
P 		675
C 103.3
(-2.6)
P 		68.3
(-2.9)
P 		59.5
(-3)
P 		66.9
(-2.9)
P 		119.9
(-2.4)
P 		715.4
(3)
P 		656
avr 94.3
(-2.7) 		68.5
(-3) 		71.1
(-3) 		67.4
(-3) 		135.9
(-2.2) 		589.3
(3) 		522
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184868_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5093
Sequence Type Exemplar sequence
Representative Public ID F12A10.1 NCBI
Target Description F12A10.1 /REP_DB=WormBase Gene ID /WP=CE01904 /TR=Q09943 /GB=AAA68294.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062647(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062647 NCBI Caenorhabditis elegans F12A10.1 (F12A10.1) mRNA, complete cds. 11/11 None
F12A10.1 ENSEMBL cdna:known chromosome:CEL140:II:5496617:5499055:1 gene:F12A10.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000033524 4/11 Cross Hyb Matching Probes None
SNAP00000033526 6/11 Cross Hyb Matching Probes None
GENEFINDER00000033530 6/11 Cross Hyb Matching Probes None
GENEFINDER00000033533 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5496614-5499053(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F12A10.1
Entrez Gene 184370 Entrez gene
SwissProt Q09943 EMBL-EBI
WormBase CE01904 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68294 Hypothetical protein F12A10.1 [Caenorhabditis elegans] ref|NP_495048.1| F12A10.1 [Caenorhabditis elegans] 4.0E-56
blast CAE59117 Hypothetical protein CBG02412 [Caenorhabditis briggsae] 4.0E-17
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495048.1 2 5-22,70-92
F12A10.1 2 5-22,70-92
Sequence
Target Sequence 
>C. ELEGANS:184868_AT
aaacgccaatggggattcggatatccgttatttggagggctcggaaactcctgggaggtg
ccaagtaatcttccaacttattggggatacaatgcaatggctggacacaatcagtctttc
ctgtttttaaatttgattatcatgcattcaactactctattcttcgttgtttttggtatc
ttggtggctgtatcgttggcgtttgatgacttgaatcatccgaagaacttacaatggaga
tcttcagaaggaagccgtgttaaacgatggggtggctggggaggcggatacccaggaggt
tacggaggtggttatccaggtggatacagtggctatggtggtggatatcctggaatgtat
ggtggtggaatgggtggaagctatgggagcagctcatggggaagctactcgagctcaaga
tctggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACGCCAATGGGGATTCGGATATC 461 125 103 Antisense
GGATATCCGTTATTTGGAGGGCTCG 268 509 121 Antisense
GGCTCGGAAACTCCTGGGAGGTGCC 79 539 140 Antisense
GGAGGTGCCAAGTAATCTTCCAACT 191 517 156 Antisense
GGCTGGACACAATCAGTCTTTCCTG 356 541 201 Antisense
TGCATTCAACTACTCTATTCTTCGT 464 579 245 Antisense
TTTGGTATCTTGGTGGCTGTATCGT 196 667 274 Antisense
GGCTGTATCGTTGGCGTTTGATGAC 155 541 288 Antisense
GTGGCTATGGTGGTGGATATCCTGG 630 491 431 Antisense
GGAGCAGCTCATGGGGAAGCTACTC 593 519 488 Antisense
AGCTACTCGAGCTCAAGATCTGGAG 589 79 505 Antisense
Sequence Source Affymetrix Proprietary Database