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[184863_s_at]

Description C32H11.12 /REP_DB=WormBase Gene ID /WP=CE19718 /TR=Q9XUH3 /GB=CAB05138.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 18      4grp_high_8_8 > 42      6grp_highest_nonorm > 60      6grp_total > 07      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8759
1 2 3 4 range
A 7881.7
(3)
P 		4905.6
(0.7)
P 		3332.7
(-0.5)
P 		97.5
(-3)
P 		7784
B 8856.1
(3)
P 		4072.7
(-0.3)
P 		3612.2
(-0.6)
P 		119.8
(-3)
P 		8736
C 6982.1
(3)
P 		4128.8
(0.5)
P 		3054.5
(-0.4)
P 		139.2
(-3)
A 		6843
avr 7906.6
(3) 		4369
(0.3) 		3333.1
(-0.5) 		118.8
(-3) 		7788

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 620
1 2 3 4 5 6 range
A 830.5
(3)
P 		269.9
(-2.4)
P 		210.1
(-3)
P 		227.9
(-2.8)
P 		311.5
(-2)
P 		234.7
(-2.8)
P 		620
B 794.9
(3)
P 		397.4
(-1.2)
P 		252
(-2.7)
P 		222.4
(-3)
P 		253.2
(-2.7)
P 		242.3
(-2.8)
P 		573
C 710.8
(3)
P 		403.6
(-0.7)
P 		217.8
(-3)
P 		214
(-3)
P 		249.1
(-2.6)
P 		217.9
(-3)
P 		497
avr 778.7
(3) 		357
(-1.5) 		226.6
(-2.9) 		221.4
(-3) 		271.3
(-2.5) 		231.6
(-2.9) 		557
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2855
1 2 3 4 range
A 2869.7
(3)
P 		1064.9
(-0.8)
P 		871.2
(-1.2)
P 		14.3
(-3)
P 		2855
B 2824.1
(3)
P 		1005.9
(-0.9)
P 		837
(-1.3)
P 		25.5
(-3)
P 		2799
C 2311.3
(3)
P 		1187.1
(0.1)
P 		1064.6
(-0.3)
P 		23.5
(-3)
A 		2288
avr 2668.4
(3) 		1086
(-0.6) 		924.3
(-1) 		21.1
(-3) 		2647

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 153.1
(3)
P 		96
(-0.2)
P 		45.8
(-3)
P 		57.8
(-2.3)
P 		94
(-0.3)
P 		57.1
(-2.4)
P 		107
B 276.6
(3)
P 		132.3
(-1)
P 		61.2
(-2.9)
P 		58.9
(-3)
P 		102.9
(-1.8)
P 		90.3
(-2.1)
P 		218
C 263.5
(3)
P 		123.1
(-0.8)
P 		55.4
(-2.7)
P 		43
(-3)
P 		80.6
(-2)
P 		72.9
(-2.2)
P 		221
avr 231.1
(3) 		117.1
(-0.8) 		54.1
(-3) 		53.2
(-3) 		92.5
(-1.7) 		73.4
(-2.3) 		178
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184863_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10272
Sequence Type Exemplar sequence
Representative Public ID C32H11.12 NCBI
Target Description C32H11.12 /REP_DB=WormBase Gene ID /WP=CE19718 /TR=Q9XUH3 /GB=CAB05138.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070073(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070073 NCBI Caenorhabditis elegans Downstream Of DAF-16 (regulated by DAF-16) family member (dod-24) (dod-24) mRNA, complete cds. 11/11 None
SNAP00000038562 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12940145:12941322:-1 11/11 None
GENEFINDER00000038572 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12940145:12941322:-1 11/11 None
C32H11.12 ENSEMBL cdna:known chromosome:CEL140:IV:12940145:12941336:-1 gene:C32H11.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12940150-12941328(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dod-24
Ensembl C32H11.12
Entrez Gene 178245 Entrez gene
SwissProt Q9XUH3 EMBL-EBI
WormBase CE19718 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05138 Hypothetical protein C32H11.12 [Caenorhabditis elegans] ref|NP_502474.1| Downstream Of DAF-16 (regulated by DAF-16) family member (dod-24) [Caenorhabditis elegans] 0.0
blast CAB05136 Hypothetical protein C32H11.10 [Caenorhabditis elegans] ref|NP_502472.1| Downstream Of DAF-16 (regulated by DAF-16) family member (dod-21) [Caenorhabditis elegans] 1.0E-109
blast CAB05135 Hypothetical protein C32H11.9 [Caenorhabditis elegans] ref|NP_502471.1| C32H11.9 [Caenorhabditis elegans] 1.0E-109
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.0E-41
Sequence
Target Sequence 
>C. ELEGANS:184863_S_AT
gggattcacaatggccgataattccaagaactatcttcttcgtcaaaccgcaatttttgg
aggagactcaacggattctacctacatcggaagtctctcacaaattttgaactctcagca
aatactcaccacctacggaaataagatttcggtatacacatttggtttggataagctcat
cgattatccactgttcatgggtcagaatcaggtggatacaatgggtcttttgaagtacaa
tggagcaaattgcccatcaactggaaattgcaatgtgacattgaatggaaattggggtgc
acatgtcgtggttgtaacaagttacgatgggcctgaaactatcaagagcttcgattcatt
cccagatactggtgtccttaatgtctacgaaaacaatgtgtccaacacaacctgcattgc
aactttgaataaattcaactacttgcaacaactgccactccaggtgaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGATTCACAATGGCCGATAATTCC 260 497 537 Antisense
TCTTCTTCGTCAAACCGCAATTTTT 405 615 570 Antisense
GAGACTCAACGGATTCTACCTACAT 101 393 599 Antisense
ACCTACATCGGAAGTCTCTCACAAA 75 87 616 Antisense
GCAAATACTCACCACCTACGGAAAT 459 317 654 Antisense
AGCTCATCGATTATCCACTGTTCAT 528 79 710 Antisense
GGGGTGCACATGTCGTGGTTGTAAC 73 499 830 Antisense
TCAAGAGCTTCGATTCATTCCCAGA 599 631 878 Antisense
GATACTGGTGTCCTTAATGTCTACG 23 423 901 Antisense
GTCCAACACAACCTGCATTGCAACT 334 477 936 Antisense
CAACAACTGCCACTCCAGGTGAATG 709 187 982 Antisense
Sequence Source Affymetrix Proprietary Database