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[184849_at]

Description T28A11.2 /REP_DB=WormBase Gene ID /WP=CE20109 /TR=P91523 /GB=AAC48113.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 66      4grp_total > 09      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 224
1 2 3 4 range
A 24.3
(-3)
A 		38.5
(-2.6)
A 		79.7
(-1.4)
P 		238.1
(3)
P 		214
B 27.3
(-3)
A 		71.5
(-1.7)
P 		44.6
(-2.5)
A 		238.6
(3)
P 		211
C 36.1
(-3)
A 		70.3
(-2)
P 		50.7
(-2.6)
P 		248.4
(3)
P 		212
avr 29.2
(-3) 		60.1
(-2.1) 		58.3
(-2.2) 		241.7
(3) 		213

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 41.4
(-2.9)
A 		47.4
(-2.6)
A 		80.3
(-0.9)
P 		43.8
(-2.8)
A 		38.9
(-3)
A 		159
(3)
P 		120
B 15.9
(-3)
A 		58.4
(-1.7)
A 		73.1
(-1.2)
P 		37.9
(-2.3)
A 		67.4
(-1.4)
P 		211.4
(3)
P 		196
C 40.9
(-3)
A 		95
(-1.4)
P 		76.4
(-2)
A 		66.7
(-2.2)
P 		52
(-2.7)
P 		247.2
(3)
P 		206
avr 32.7
(-3) 		66.9
(-1.8) 		76.6
(-1.5) 		49.5
(-2.4) 		52.8
(-2.3) 		205.9
(3) 		173
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 8.8
(-2.9)
A 		8.4
(-3)
A 		20.8
(-0.2)
P 		34.9
(3)
P 		27
B 8.7
(-3)
A 		17.7
(-1.7)
P 		10.3
(-2.8)
A 		50.8
(3)
P 		42
C 12
(-3)
A 		20.2
(-1.4)
P 		17.7
(-1.9)
P 		41.9
(3)
P 		30
avr 9.8
(-3) 		15.4
(-2) 		16.3
(-1.8) 		42.5
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 7.6
(-3)
A 		16.9
(-1.2)
A 		17.5
(-1.1)
P 		11.1
(-2.3)
A 		11.7
(-2.2)
A 		38.7
(3)
P 		31
B 5.5
(-3)
A 		19.5
(-1.9)
A 		17.8
(-2)
P 		10
(-2.6)
A 		27.4
(-1.2)
P 		78.8
(3)
P 		73
C 15.2
(-2.8)
A 		29
(-1.6)
P 		19.4
(-2.5)
A 		13.4
(-3)
P 		16.8
(-2.7)
P 		82.7
(3)
P 		69
avr 9.4
(-3) 		21.8
(-1.7) 		18.2
(-2.1) 		11.5
(-2.8) 		18.6
(-2) 		66.7
(3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184849_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15734
Sequence Type Exemplar sequence
Representative Public ID T28A11.2 NCBI
Target Description T28A11.2 /REP_DB=WormBase Gene ID /WP=CE20109 /TR=P91523 /GB=AAC48113.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071505(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071505 NCBI Caenorhabditis elegans T28A11.2 (T28A11.2) mRNA, complete cds. 11/11 A 
SNAP00000028219 ENSEMBL cdna:SNAP chromosome:CEL140:V:3281654:3282469:1 11/11 None
GENEFINDER00000028229 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3281654:3284474:1 9/11 None
T28A11.2 ENSEMBL cdna:known chromosome:CEL140:V:3280208:3282469:1 gene:T28A11.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3280207-3282469(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28A11.2
Entrez Gene 189009 Entrez gene
SwissProt P91523 EMBL-EBI
WormBase CE20109 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503906 T28A11.2 [Caenorhabditis elegans] gb|AAC48113.2| Hypothetical protein T28A11.2 [Caenorhabditis elegans] 0.0
blast AAC04396 Hypothetical protein C17B7.2 [Caenorhabditis elegans] ref|NP_503930.1| C17B7.2 [Caenorhabditis elegans] 1.0E-177
blast NP_503907 Seven TM Receptor family member (str-64) [Caenorhabditis elegans] gb|AAC48112.1| Seven tm receptor protein 64 [Caenorhabditis elegans] 1.0E-174
blast AAC04405 Seven tm receptor protein 63 [Caenorhabditis elegans] ref|NP_503931.1| Seven TM Receptor family member (str-63) [Caenorhabditis elegans] 1.0E-168
blast NP_503906 T28A11.2 [Caenorhabditis elegans] gb|AAC48113.2| Hypothetical protein T28A11.2 [Caenorhabditis elegans] 1.0E-116
blast NP_503906 T28A11.2 [Caenorhabditis elegans] gb|AAC48113.2| Hypothetical protein T28A11.2 [Caenorhabditis elegans] 1.0E-103
blast AAC04396 Hypothetical protein C17B7.2 [Caenorhabditis elegans] ref|NP_503930.1| C17B7.2 [Caenorhabditis elegans] 2.0E-79
blast NP_504867 F10G2.1 [Caenorhabditis elegans] gb|AAG24056.2| Hypothetical protein F10G2.1 [Caenorhabditis elegans] 1.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 1.6E-62
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 1.5E-52
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 1.5E-52
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 3.8E-47
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000028229 6 190-212,232-254,275-297,343-365,389-411,426-448
Sequence
Target Sequence 
>C. ELEGANS:184849_AT
gttatcctgcttgtcatggtgttcctgtgttctgtcgagtgtgcaagtcaaattgaacaa
ctgaaaggtacaccctgcactgaggctgatgcatttctggaatattcttgtcttttgagc
ttgaaagctttcctggggaagattgatgaagtggatgtaaggaataagaacgacctcaac
aaattcgacaaatcttgcacccaattcactccatgtttgagaacccttcaatgtggccca
gaacatcctgcaaggaaacaaaagcttaaattgatcagaacctattgtgagagtattgtc
tttattagcaaaaattttacggattgcgatgcgaagcttgagagcaagaattccacgtgc
tacactgcttgggacccgttcctggaaaattatgaagaagaactagatgagaagaagaaa
aaacaaatgcttcaagaaaactgcaaagactattttggaaaagacaactgtatgaagaag
gagattacggagacatgtggtgaagccgaatgggtgaaattccaagctaatttcatcgga
gtcggcaatatgctcagtaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTATCCTGCTTGTCATGGTGTTCC 474 447 622 Antisense
TGTCATGGTGTTCCTGTGTTCTGTC 295 559 633 Antisense
GTGTTCTGTCGAGTGTGCAAGTCAA 634 485 648 Antisense
ACAACTGAAAGGTACACCCTGCACT 204 111 678 Antisense
TACACCCTGCACTGAGGCTGATGCA 336 643 690 Antisense
GAGGCTGATGCATTTCTGGAATATT 91 407 703 Antisense
GGAATATTCTTGTCTTTTGAGCTTG 403 533 720 Antisense
GAGCTTGAAAGCTTTCCTGGGGAAG 171 383 738 Antisense
TACGGATTGCGATGCGAAGCTTGAG 592 647 939 Antisense
CCAAGCTAATTTCATCGGAGTCGGC 84 271 1143 Antisense
GGAGTCGGCAATATGCTCAGTAACT 465 517 1159 Antisense
Sequence Source Affymetrix Proprietary Database