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[184815_at]

Description F28E10.4 /REP_DB=WormBase Gene ID /WP=CE04461 /TR=Q19883 /GB=AAA81502.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 24      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 38.2
(-3)
A 		49.9
(-1.1)
A 		55.3
(-0.3)
A 		76
(3)
A 		38
B 24.8
(-3)
A 		55.9
(3)
A 		44.8
(0.9)
A 		39.7
(-0.1)
A 		31
C 45.8
(2.4)
A 		25.2
(-3)
A 		44.3
(2)
A 		48.2
(3)
A 		23
avr 36.3
(-3) 		43.7
(-0.6) 		48.1
(0.9) 		54.6
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 98.1
(0.5)
A 		34.1
(-3)
A 		33.5
(-3)
A 		55.1
(-1.8)
A 		121.4
(1.8)
A 		144.1
(3)
A 		111
B 25.6
(-3)
A 		37.3
(-1.8)
A 		41.2
(-1.5)
A 		58.8
(0.3)
A 		83.9
(2.8)
A 		86.3
(3)
A 		61
C 23.3
(-3)
A 		56.7
(0)
A 		72.9
(1.5)
A 		28.9
(-2.5)
A 		84.8
(2.6)
A 		89.6
(3)
A 		66
avr 49
(-2.4) 		42.7
(-3) 		49.2
(-2.4) 		47.6
(-2.5) 		96.7
(2.1) 		106.7
(3) 		64
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 13.9
(2.2)
A 		10.8
(-3)
A 		14.4
(3)
A 		11.1
(-2.5)
A 		4
B 7.9
(-3)
A 		13.8
(3)
A 		10.4
(-0.5)
A 		8.5
(-2.4)
A 		6
C 15.2
(2.9)
A 		7.3
(-3)
A 		15.4
(3)
A 		8.1
(-2.4)
A 		8
avr 12.3
(1.4) 		10.6
(-1) 		13.4
(3) 		9.2
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 18.1
(-0.8)
A 		12.1
(-2)
A 		7.3
(-3)
A 		14
(-1.6)
A 		36.6
(3)
A 		35.1
(2.7)
A 		29
B 8.9
(-3)
A 		12.4
(-2.2)
A 		10
(-2.7)
A 		15.6
(-1.4)
A 		34.1
(3)
A 		32.2
(2.5)
A 		25
C 8.6
(-2.3)
A 		17.3
(-0.1)
A 		18.6
(0.2)
A 		5.8
(-3)
A 		27.4
(2.4)
A 		30
(3)
A 		24
avr 11.9
(-3) 		13.9
(-2.4) 		12
(-2.9) 		11.8
(-3) 		32.7
(3) 		32.4
(2.9) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184815_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11021
Sequence Type Exemplar sequence
Representative Public ID F28E10.4 NCBI
Target Description F28E10.4 /REP_DB=WormBase Gene ID /WP=CE04461 /TR=Q19883 /GB=AAA81502.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068189(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068189 NCBI Caenorhabditis elegans F28E10.4 (F28E10.4) mRNA, complete cds. 11/11 None
SNAP00000006734 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4566011:4567317:-1 11/11 None
GENEFINDER00000006741 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4566011:4567317:-1 11/11 None
F28E10.4 ENSEMBL cdna:known chromosome:CEL140:IV:4566011:4566998:-1 gene:F28E10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4566016-4567004(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28E10.4
Entrez Gene 185067 Entrez gene
SwissProt Q19883 EMBL-EBI
WormBase CE04461 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_500590 F28E10.4 [Caenorhabditis elegans] gb|AAA81502.1| Hypothetical protein F28E10.4 [Caenorhabditis elegans] 1.0E-129
blast NP_500590 F28E10.4 [Caenorhabditis elegans] gb|AAA81502.1| Hypothetical protein F28E10.4 [Caenorhabditis elegans] 1.0E-125
blast CAE64422 Hypothetical protein CBG09116 [Caenorhabditis briggsae] 3.0E-96
blast CAE64422 Hypothetical protein CBG09116 [Caenorhabditis briggsae] 6.0E-77
blast AAF99885 Hypothetical protein C46H11.6 [Caenorhabditis elegans] ref|NP_491502.1| C46H11.6 [Caenorhabditis elegans] 2.0E-65
Sequence
Target Sequence 
>C. ELEGANS:184815_AT
cacgtcagtcggcctgaagcttggaatcatcaaaagacgcgcatttgtcaccaaagtcga
cccgaacactatcgcttcttcattttttggatgcggtgatagcattatggatatgagcgg
tgcaccgattccgatgactgataatccggataatttcattcgcgagcatctctcgagact
gtcaactggagcaaaagtgtcatttctggtggagagaccgatttcggtggctatggcgaa
gcagtatcaaaagttcatcgagtcgattacatatgatgattcggaagtggagatggccca
ggatgttatagagatcggaagggaagccagtaatatgcactttatgattctgaagaaact
agtcactccgagcatcttgtcctccgatgtcagaagaagaaagaagtcgaacaacaaaac
aagtgaatcgacggaggggtccatcactatttcgagtgcatcgactgagatgaagatcac
cagtgacgtcacagatttcgatgatttgaagccagttgagtcgaaaagtcacgcagctgg
atcctct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACGTCAGTCGGCCTGAAGCTTGGA 159 197 249 Antisense
GACGCGCATTTGTCACCAAAGTCGA 182 381 284 Antisense
CAAAGTCGACCCGAACACTATCGCT 218 191 300 Antisense
GAACACTATCGCTTCTTCATTTTTT 96 353 312 Antisense
GTGCACCGATTCCGATGACTGATAA 188 481 368 Antisense
ATAATTTCATTCGCGAGCATCTCTC 148 27 398 Antisense
GAGCATCTCTCGAGACTGTCAACTG 401 389 412 Antisense
GAAACTAGTCACTCCGAGCATCTTG 464 357 603 Antisense
CATCTTGTCCTCCGATGTCAGAAGA 418 213 621 Antisense
GAATCGACGGAGGGGTCCATCACTA 639 329 673 Antisense
GAAAAGTCACGCAGCTGGATCCTCT 568 353 771 Antisense
Sequence Source Affymetrix Proprietary Database