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[184803_s_at]

Description F35G12.12 /REP_DB=WormBase Gene ID /WP=CE20752 /TR=Q20058 /GB=CAA86334.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 76      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 92      6grp_total > 67      4grp_total > 03      6grp_highest_sub > In      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 268
1 2 3 4 range
A 183.9
(-3)
P 		203.3
(-2.4)
P 		186.4
(-2.9)
P 		376.4
(3)
P 		193
B 191.8
(-2)
P 		177.6
(-2.7)
P 		170.7
(-3)
P 		294.6
(3)
P 		124
C 215.3
(0)
P 		108.3
(-3)
P 		195.8
(-0.5)
P 		321.4
(3)
P 		213
avr 197
(-1.8) 		163.1
(-3) 		184.3
(-2.2) 		330.8
(3) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 404
1 2 3 4 5 6 range
A 149.9
(-3)
P 		176.2
(-2.3)
P 		287.3
(0.6)
P 		239.9
(-0.6)
P 		243.5
(-0.5)
P 		376.3
(3)
P 		226
B 288.1
(-1.8)
P 		243.7
(-2.6)
P 		337.6
(-0.9)
P 		223.9
(-3)
P 		301.3
(-1.6)
P 		553.5
(3)
P 		330
C 242.5
(-2.8)
P 		309.1
(-1)
P 		236.5
(-3)
P 		303.8
(-1.1)
P 		246.3
(-2.7)
P 		453.6
(3)
P 		217
avr 226.8
(-3) 		243
(-2.6) 		287.1
(-1.5) 		255.9
(-2.3) 		263.7
(-2.1) 		461.1
(3) 		234
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 67
(3)
P 		44.1
(-3)
P 		48.7
(-1.8)
P 		55.1
(-0.1)
P 		23
B 61.2
(2.6)
P 		43.9
(-1.9)
P 		39.6
(-3)
P 		62.8
(3)
P 		23
C 71.3
(3)
P 		31.1
(-3)
P 		68.2
(2.5)
P 		54.2
(0.4)
P 		40
avr 66.5
(3) 		39.7
(-3) 		52.2
(-0.2) 		57.4
(1) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 179
1 2 3 4 5 6 range
A 27.6
(-3)
P 		62.7
(0.3)
P 		62.6
(0.3)
P 		60.8
(0.1)
P 		73.5
(1.3)
P 		91.6
(3)
P 		64
B 100.2
(-1.3)
P 		81.1
(-2.1)
P 		82
(-2.1)
P 		59.3
(-3)
P 		122.4
(-0.4)
P 		206.4
(3)
P 		147
C 89.9
(-1.1)
P 		94.3
(-0.8)
P 		60.2
(-3)
P 		61.1
(-2.9)
P 		79.7
(-1.7)
P 		151.9
(3)
P 		92
avr 72.6
(-2.2) 		79.4
(-1.7) 		68.3
(-2.5) 		60.4
(-3) 		91.9
(-0.9) 		150
(3) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184803_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7460
Sequence Type Exemplar sequence
Representative Public ID F35G12.12 NCBI
Target Description F35G12.12 /REP_DB=WormBase Gene ID /WP=CE20752 /TR=Q20058 /GB=CAA86334.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065531(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065531 NCBI Caenorhabditis elegans F35G12.12 (F35G12.12) mRNA, complete cds. 11/11 A 
SNAP00000042239 ENSEMBL cdna:SNAP chromosome:CEL140:III:4581760:4583705:1 11/11 None
GENEFINDER00000042253 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4581760:4583705:1 11/11 None
F35G12.12.1 ENSEMBL cdna:known chromosome:CEL140:III:4581758:4583850:1 gene:F35G12.12 11/11 None
F35G12.12.2 ENSEMBL cdna:known chromosome:CEL140:III:4581760:4583850:1 gene:F35G12.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4581759-4583705(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35G12.12
Entrez Gene 175601 Entrez gene
SwissProt Q20058 EMBL-EBI
WormBase CE31499 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86334 Hypothetical protein F35G12.12 [Caenorhabditis elegans] ref|NP_497932.2| F35G12.12 [Caenorhabditis elegans] 0.0
blast CAE71180 Hypothetical protein CBG18037 [Caenorhabditis briggsae] 1.0E-173
Sequence
Target Sequence 
>C. ELEGANS:184803_S_AT
gattcattgactgggcattggacgataaatcagaagataatccagaatacgaaattcgca
aacgtcaaattattcatcgaatcattgaattgtcggagaatacaaagagcgccgaagtac
tcaaagctgatccagaactgattggccgtctcaagaaatatctgcaaccggctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCATTGACTGGGCATTGGACGA 341 431 1382 Antisense
TTGACTGGGCATTGGACGATAAATC 109 703 1388 Antisense
ATACGAAATTCGCAAACGTCAAATT 465 1 1428 Antisense
AAACGTCAAATTATTCATCGAATCA 234 127 1441 Antisense
GAATCATTGAATTGTCGGAGAATAC 657 327 1460 Antisense
GGAGAATACAAAGAGCGCCGAAGTA 687 519 1476 Antisense
GCGCCGAAGTACTCAAAGCTGATCC 267 287 1490 Antisense
GCTGATCCAGAACTGATTGGCCGTC 529 295 1507 Antisense
CCAGAACTGATTGGCCGTCTCAAGA 710 271 1513 Antisense
GGCCGTCTCAAGAAATATCTGCAAC 473 543 1525 Antisense
CTCAAGAAATATCTGCAACCGGCTG 194 227 1531 Antisense
Sequence Source Affymetrix Proprietary Database