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[184789_at]

Description Y57A10A.8 /REP_DB=WormBase Gene ID /WP=CE22613 /TR=Q9NA84 /GB=CAB55013.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 86      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 91      6grp_total > 88      4grp_total > 15      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 148.9
(2.1)
P 		62.3
(-3)
P 		108.1
(-0.3)
P 		163.7
(3)
P 		101
B 177.9
(3)
P 		68.1
(-3)
P 		109.4
(-0.7)
P 		152.9
(1.6)
P 		110
C 171.9
(3)
P 		123
(-0.8)
P 		94.1
(-3)
P 		171
(2.9)
P 		78
avr 166.2
(3) 		84.5
(-3) 		103.9
(-1.6) 		162.5
(2.7) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 172.8
(-0.8)
P 		131.2
(-3)
P 		181.2
(-0.4)
P 		165.3
(-1.2)
P 		205.1
(0.9)
P 		245.2
(3)
P 		114
B 157.6
(-3)
P 		179.1
(-1.9)
P 		177.4
(-2)
P 		186.4
(-1.5)
P 		214.2
(-0.1)
P 		273.3
(3)
P 		116
C 114
(-3)
P 		193.1
(-0.3)
P 		134.2
(-2.3)
P 		118.5
(-2.8)
P 		178.7
(-0.8)
P 		290.1
(3)
P 		176
avr 148.1
(-3) 		167.8
(-2) 		164.3
(-2.2) 		156.7
(-2.6) 		199.3
(-0.5) 		269.5
(3) 		121
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 54.2
(3)
P 		13.5
(-3)
P 		28.3
(-0.8)
P 		24
(-1.5)
P 		41
B 56.7
(3)
P 		16.8
(-3)
P 		25.4
(-1.7)
P 		32.6
(-0.6)
P 		40
C 56.9
(3)
P 		35.4
(-1.6)
P 		32.8
(-2.1)
P 		28.8
(-3)
P 		28
avr 55.9
(3) 		21.9
(-3) 		28.8
(-1.8) 		28.5
(-1.8) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 31.9
(-3)
P 		46.7
(-0)
P 		39.5
(-1.5)
P 		41.9
(-1)
P 		61.9
(3)
P 		59.7
(2.6)
P 		30
B 54.8
(-1.8)
P 		59.6
(-1.3)
P 		43.1
(-3)
P 		49.4
(-2.4)
P 		87
(1.5)
P 		101.9
(3)
P 		59
C 42.2
(-1.5)
P 		58.9
(-0.1)
P 		34.2
(-2.1)
P 		23.8
(-3)
P 		57.8
(-0.2)
P 		97.1
(3)
P 		73
avr 43
(-2.4) 		55.1
(-0.9) 		38.9
(-2.9) 		38.4
(-3) 		68.9
(0.8) 		86.2
(3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184789_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2720
Sequence Type Exemplar sequence
Representative Public ID Y57A10A.8 NCBI
Target Description Y57A10A.8 /REP_DB=WormBase Gene ID /WP=CE22613 /TR=Q9NA84 /GB=CAB55013.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064187(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064187 NCBI Caenorhabditis elegans Y57A10A.8 (Y57A10A.8) mRNA, complete cds. 11/11 None
SNAP00000026020 ENSEMBL cdna:SNAP chromosome:CEL140:II:12165193:12175909:1 10/11 None
GENEFINDER00000026044 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12164750:12170541:1 11/11 None
Y57A10A.8 ENSEMBL cdna:known chromosome:CEL140:II:12164742:12170622:1 gene:Y57A10A.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12164746-12170538(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y57A10A.8
Entrez Gene 190336 Entrez gene
SwissProt Q9NA84 EMBL-EBI
WormBase CE35687 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB55013 Hypothetical protein Y57A10A.8 [Caenorhabditis elegans] ref|NP_496588.2| Y57A10A.8 [Caenorhabditis elegans] 0.0
blast CAB55014 Hypothetical protein Y57A10A.11 [Caenorhabditis elegans] ref|NP_496589.1| ROLler: helically twisted, animals roll when moving family member (rol-1) [Caenorhabditis elegans] 1.0E-139
blast CAE73466 Hypothetical protein CBG20917 [Caenorhabditis briggsae] 1.0E-110
blast CAB07288 Hypothetical protein T28A8.4 [Caenorhabditis elegans] ref|NP_499794.1| T28A8.4 [Caenorhabditis elegans] 5.0E-90
blast CAB55010 Hypothetical protein Y57A10A.5 [Caenorhabditis elegans] ref|NP_496585.1| Y57A10A.5 [Caenorhabditis elegans] 7.0E-87
Protein Domains
Database ID Description E-Value
Pfam IPR006570 EMBL-EBI SPK 1.1E-66
Pfam IPR006570 EMBL-EBI SPK 1.9E-59
Pfam IPR006570 EMBL-EBI SPK 1.1E-35
Pfam IPR006570 EMBL-EBI SPK 1.9E-59
Pfam IPR002486 EMBL-EBI Nematode cuticle collagen, N-terminal 4.7E-7
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.2E-4
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.2E-8
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.0E-11
Pfam IPR006570 EMBL-EBI SPK 3.9E-54
Sequence
Target Sequence 
>C. ELEGANS:184789_AT
gaattcgttgaatcactgccaggaaaaccgaaacttgaacccattgattacgactacgac
caatattttaatagagatgtatcatataataattatgatcaagaagaagttgaaaatcga
aatgtcagattatcgattgctagctcaatgccttcactggagtcgaatgttattaaattg
aatgatttcctgaaagaagttcgcaaattcctgtgctttctcgaaatttcggaactcgac
gagctcaagcatcagatcaaagaatcggttgttcagactgctggcgctaatgaggtagca
aagattagaaatctggaaatcagcgatctttgtacattactcgatgcattcttcttctcg
atcaatcaaaaaaccagatcaactattccaaatgattccactatgccggtcaatgtattc
ctcatcaaattcaaatgcttcctccttggaatggaatctcctgaatttctagatcttcag
gagaaggttcaaaggaagcttgacgagtcggatatcacggaaaagattcttcaactgagc
gacatccaacac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCGTTGAATCACTGCCAGGAA 707 323 952 Antisense
TGAACCCATTGATTACGACTACGAC 345 573 987 Antisense
GTCAGATTATCGATTGCTAGCTCAA 299 467 1075 Antisense
CTAGCTCAATGCCTTCACTGGAGTC 662 223 1091 Antisense
AGTTCGCAAATTCCTGTGCTTTCTC 231 63 1149 Antisense
AACTCGACGAGCTCAAGCATCAGAT 324 141 1184 Antisense
GAATCGGTTGTTCAGACTGCTGGCG 511 329 1213 Antisense
GCGATCTTTGTACATTACTCGATGC 434 291 1274 Antisense
ATTCCACTATGCCGGTCAATGTATT 432 7 1346 Antisense
GCTTCCTCCTTGGAATGGAATCTCC 271 307 1388 Antisense
TCTTCAACTGAGCGACATCCAACAC 3 617 1479 Antisense
Sequence Source Affymetrix Proprietary Database