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[184773_s_at]

Description Y66H1B.3 /REP_DB=WormBase Gene ID /WP=CE20347 /TR=Q9TYW4 /GB=AAC69000.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 13      4grp_highest_8_8 > 54      4grp_high_8_8 > 18      6grp_highest_nonorm > 70      6grp_total > 12      4grp_total > 22      6grp_highest_sub > 02      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 253
1 2 3 4 range
A 363.4
(2.3)
P 		147.1
(-3)
P 		216.9
(-1.3)
P 		390
(3)
P 		243
B 345.6
(3)
P 		245.5
(0.1)
P 		137.4
(-3)
P 		325.7
(2.4)
P 		208
C 304.7
(3)
P 		280.7
(2)
P 		167.4
(-3)
P 		282.2
(2)
P 		137
avr 337.9
(3) 		224.4
(-1.2) 		173.9
(-3) 		332.6
(2.8) 		164

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 297
1 2 3 4 5 6 range
A 422.7
(3)
P 		380.4
(1.8)
P 		277.6
(-1)
P 		252.1
(-1.6)
P 		244.5
(-1.9)
P 		202.3
(-3)
P 		220
B 244
(-0.1)
P 		367.7
(3)
P 		350.4
(2.6)
P 		205.6
(-1)
P 		126.1
(-3)
P 		172.6
(-1.8)
P 		242
C 263.9
(0)
P 		377.4
(3)
P 		147.9
(-3)
P 		247
(-0.4)
P 		183.7
(-2.1)
P 		194.6
(-1.8)
P 		230
avr 310.2
(1) 		375.2
(3) 		258.6
(-0.7) 		234.9
(-1.4) 		184.8
(-3) 		189.8
(-2.8) 		190
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 132.3
(3)
P 		31.9
(-3)
P 		56.7
(-1.5)
P 		57.1
(-1.5)
P 		100
B 110.2
(3)
P 		60.6
(-0.8)
P 		31.8
(-3)
P 		69.4
(-0.1)
P 		78
C 100.9
(3)
P 		80.7
(0.7)
P 		58.3
(-1.8)
P 		47.6
(-3)
P 		53
avr 114.5
(3) 		57.7
(-2.2) 		48.9
(-3) 		58
(-2.2) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 77.9
(-1)
P 		135.3
(3)
P 		60.5
(-2.2)
P 		63.9
(-2)
P 		73.8
(-1.3)
P 		49.3
(-3)
P 		86
B 84.9
(-0.2)
P 		122.4
(3)
P 		85.1
(-0.1)
P 		54.5
(-2.7)
P 		51.2
(-3)
P 		64.4
(-1.9)
P 		71
C 97.8
(1.7)
P 		115.1
(3)
P 		37.6
(-3)
P 		49.7
(-2.1)
P 		59.4
(-1.3)
P 		65.1
(-0.9)
P 		78
avr 86.9
(-0.3) 		124.3
(3) 		61.1
(-2.6) 		56
(-3) 		61.5
(-2.5) 		59.6
(-2.7) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184773_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10688
Sequence Type Exemplar sequence
Representative Public ID Y66H1B.3 NCBI
Target Description Y66H1B.3 /REP_DB=WormBase Gene ID /WP=CE20347 /TR=Q9TYW4 /GB=AAC69000.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067511(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067511 NCBI Caenorhabditis elegans Y66H1B.3 (Y66H1B.3) mRNA, complete cds. 11/11 None
SNAP00000025523 ENSEMBL cdna:SNAP chromosome:CEL140:IV:370363:376012:-1 11/11 None
GENEFINDER00000025526 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:358809:376012:-1 11/11 None
Y66H1B.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:370293:376064:-1 gene:Y66H1B.3 11/11 None
Y66H1B.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:370293:376062:-1 gene:Y66H1B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:370362-376012(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y66H1B.3
Entrez Gene 176856 Entrez gene
SwissProt Q9TYW4 EMBL-EBI
WormBase CE20347 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HG-U133_PLUS_2:207876_S_AT filamin C, gamma (actin binding protein 280) hs
HG-U133A:207876_S_AT filamin C, gamma (actin binding protein 280) hs
HG-FOCUS:207876_S_AT filamin C, gamma (actin binding protein 280) hs
HG-U133A_2:207876_S_AT filamin C, gamma (actin binding protein 280) hs
HG-U95AV2:35330_AT filamin C, gamma (actin binding protein 280) hs
HU35KSUBC:RC_AA404397_AT filamin C, gamma (actin binding protein 280) hs
HUGENEFL:X70083_AT filamin C, gamma (actin binding protein 280) hs
U133_X3P:G4557596_3P_A_AT filamin C, gamma (actin binding protein 280) hs
MU11KSUBB:MSA.36954.0_S_AT filamin C, gamma (actin binding protein 280) mm
MOUSE430_2:1449073_AT filamin C, gamma (actin binding protein 280) mm
MOE430A:1449073_AT filamin C, gamma (actin binding protein 280) mm
MOUSE430A_2:1449073_AT filamin C, gamma (actin binding protein 280) mm
MOUSE430_2:1447812_X_AT filamin C, gamma (actin binding protein 280) mm
MOE430B:1447812_X_AT filamin C, gamma (actin binding protein 280) mm
MU11KSUBB:MSA.14580.0_S_AT filamin C, gamma (actin binding protein 280) mm
MU11KSUBB:MSA.38029.0_S_AT filamin C, gamma (actin binding protein 280) mm
MU11KSUBB:MSA.4759.0_S_AT filamin C, gamma (actin binding protein 280) mm
MU11KSUBB:MSA.8926.0_S_AT filamin C, gamma (actin binding protein 280) mm
MG-U74BV2:106757_F_AT filamin C, gamma (actin binding protein 280) mm
MG-U74CV2:167035_R_AT filamin C, gamma (actin binding protein 280) mm
MU19KSUBC:TC40726_AT Filamin C, gamma (actin binding protein 280), mRNA (cDNA clone IMAGE:6824907) mm
RG-U34B:RC_AI008162_AT filamin C, gamma (actin binding protein 280) (predicted) rn
RAE230B:1396085_AT filamin C, gamma (actin binding protein 280) (predicted) rn
RAT230_2:1396085_AT filamin C, gamma (actin binding protein 280) (predicted) rn
RAE230A:1388496_AT Filamin C, gamma (actin binding protein 280) (predicted) rn
RAT230_2:1388496_AT Filamin C, gamma (actin binding protein 280) (predicted) rn
RG-U34A:RC_AA799773_AT Filamin C, gamma (actin binding protein 280) (predicted) rn
RG-U34A:RC_AA799773_G_AT Filamin C, gamma (actin binding protein 280) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAC69000 Hypothetical protein Y66H1B.3 [Caenorhabditis elegans] ref|NP_499912.1| Y66H1B.3 [Caenorhabditis elegans] 0.0
blast CAE65554 Hypothetical protein CBG10542 [Caenorhabditis briggsae] 0.0
blast CAE65553 Hypothetical protein CBG10541 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.2E-28
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.8E-27
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 1.5E-19
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 1.9E-24
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 2.4E-9
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 1.9E-20
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 2.4E-9
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 1.9E-20
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 1.9E-26
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 4.1E-5
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 7.0E-30
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 1.3E-6
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 6.7E-26
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 3.1E-8
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 1.6E-29
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 6.8E-10
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 5.2E-23
Pfam IPR001298 EMBL-EBI Filamin/ABP280 repeat 2.0E-12
Sequence
Target Sequence 
>C. ELEGANS:184773_S_AT
gactccgactggattccgtgttaggttcactccgtcagaagttggaaactacacaattga
cgtcacttatcaggatattcctattgagagatcgccatttacactgcaatctgtggaagg
acctaaaaatcaagatgacagtgaaatcttagatggggaaaatcaagaaaatcccggaga
cagaacactcgccgaagcagagtatttagttttcggttcggggccaccttgtgctgatat
ggtcacggtgtctgggcctggattagggcctcttgtagctcaacgatcaacatatgtatc
aattgacactacaaacgcaggattcggagatatcgatgtttatgttgatggtcctactcg
tacaccgttgcattgtgtggacaatcaagatggaatactgaaaatgagcttcacaccaaa
acagccaggactatattatcttcgtgtaatgtttgataatgaacatgtaccgggaagtcc
atttcaaattgtggcagtggctgcattacttggaaatccattggatcaacgtagtaaaac
ggagagcccatactcatctatttcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTCCGACTGGATTCCGTGTTAGG 540 365 2655 Antisense
CGTGTTAGGTTCACTCCGTCAGAAG 214 101 2671 Antisense
GAAACTACACAATTGACGTCACTTA 284 357 2699 Antisense
GATCGCCATTTACACTGCAATCTGT 336 417 2744 Antisense
AATCCCGGAGACAGAACACTCGCCG 327 151 2824 Antisense
GGCCACCTTGTGCTGATATGGTCAC 170 543 2876 Antisense
ACGGTGTCTGGGCCTGGATTAGGGC 632 95 2899 Antisense
GGATTAGGGCCTCTTGTAGCTCAAC 20 509 2914 Antisense
GTTTATGTTGATGGTCCTACTCGTA 320 449 2992 Antisense
CCTACTCGTACACCGTTGCATTGTG 645 267 3007 Antisense
GGAGAGCCCATACTCATCTATTTCC 438 519 3195 Antisense
Sequence Source Affymetrix Proprietary Database