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[184767_s_at]

Description C27A12.9 /REP_DB=WormBase Gene ID /WP=CE08418 /TR=O01966 /GB=AAB93646.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      4grp_high_8_8 > 03      6grp_highest_nonorm > 91      6grp_total > 77      4grp_total > 06      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 123.5
(0.2)
P 		23.4
(-3)
A 		124.6
(0.3)
P 		208.7
(3)
A 		185
B 104.5
(1)
P 		73.1
(-1)
A 		42.2
(-3)
P 		136.1
(3)
P 		94
C 148.7
(0.6)
P 		94.9
(-3)
P 		116.8
(-1.5)
P 		184.1
(3)
P 		89
avr 125.6
(0.3) 		63.8
(-3) 		94.5
(-1.4) 		176.3
(3) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 88.9
(-3)
P 		185.7
(-0.6)
P 		131.3
(-2)
P 		176.7
(-0.8)
P 		156.3
(-1.3)
P 		333.5
(3)
P 		245
B 138.9
(-1.4)
P 		124.9
(-2)
P 		97.7
(-3)
P 		164.2
(-0.5)
P 		228
(1.9)
P 		255.8
(3)
P 		158
C 99.4
(-3)
P 		156.1
(-1.3)
P 		155.3
(-1.3)
P 		154.6
(-1.3)
P 		139.6
(-1.8)
P 		298
(3)
P 		199
avr 109.1
(-3) 		155.6
(-1.5) 		128.1
(-2.4) 		165.2
(-1.2) 		174.6
(-0.9) 		295.8
(3) 		187
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 45
(3)
P 		5.1
(-3)
A 		32.6
(1.1)
P 		30.5
(0.8)
A 		40
B 33.3
(3)
P 		18
(-0.9)
A 		9.8
(-3)
P 		29
(1.9)
P 		24
C 49.2
(3)
P 		27.3
(-3)
P 		40.7
(0.7)
P 		31
(-2)
P 		22
avr 42.5
(3) 		16.8
(-3) 		27.7
(-0.5) 		30.2
(0.1) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 16.4
(-3)
P 		66
(1.6)
P 		28.6
(-1.9)
P 		44.8
(-0.4)
P 		47.2
(-0.1)
P 		81.2
(3)
P 		65
B 48.3
(-0.9)
P 		41.6
(-1.5)
P 		23.7
(-3)
P 		43.5
(-1.3)
P 		92.6
(2.8)
P 		95.4
(3)
P 		72
C 36.8
(-2.5)
P 		47.6
(-1.6)
P 		39.5
(-2.3)
P 		31.1
(-3)
P 		45.2
(-1.8)
P 		99.7
(3)
P 		69
avr 33.8
(-2.7) 		51.7
(-0.9) 		30.6
(-3) 		39.8
(-2.1) 		61.7
(0) 		92.1
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184767_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.396
Sequence Type Exemplar sequence
Representative Public ID C27A12.9 NCBI
Target Description C27A12.9 /REP_DB=WormBase Gene ID /WP=CE08418 /TR=O01966 /GB=AAB93646.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059349(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059349 NCBI Caenorhabditis elegans C27A12.9 (C27A12.9) mRNA, complete cds. 11/11 None
SNAP00000016774 ENSEMBL cdna:SNAP chromosome:CEL140:I:6070011:6071726:-1 11/11 None
GENEFINDER00000016780 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6070011:6071774:-1 11/11 None
C27A12.9 ENSEMBL cdna:known chromosome:CEL140:I:6069915:6076167:-1 gene:C27A12.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6070010-6076167(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27A12.9
Entrez Gene 172285 Entrez gene
SwissProt O01966 EMBL-EBI
WormBase CE36272 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250085_AT phosphatidylinositolglycan class O (PIG-O) family protein at
CANINE_2:CFAAFFX.3922.1.S1_AT similar to phosphatidylinositol glycan, class O isoform 2 cfa
CANINE_2:CFAAFFX.3922.1.S1_S_AT similar to phosphatidylinositol glycan, class O isoform 2 cfa
DROSOPHILA_2:1632403_AT dm
DROSGENOME1:141723_AT dm
HG-U133_PLUS_2:209998_AT phosphatidylinositol glycan, class O hs
HG-U133A:209998_AT phosphatidylinositol glycan, class O hs
HG-U133A_2:209998_AT phosphatidylinositol glycan, class O hs
HG-U133_PLUS_2:214991_S_AT phosphatidylinositol glycan, class O hs
HG-U133A_2:214991_S_AT phosphatidylinositol glycan, class O hs
HG-U133A:214991_S_AT phosphatidylinositol glycan, class O hs
HG-U95C:62953_AT phosphatidylinositol glycan, class O hs
HU35KSUBB:RC_T65159_AT phosphatidylinositol glycan, class O hs
HG-U95AV2:32409_AT phosphatidylinositol glycan, class O hs
U133_X3P:G12654408_3P_AT phosphatidylinositol glycan, class O hs
U133_X3P:HS.85044.0.S1_3P_S_AT phosphatidylinositol glycan, class O hs
HG-U133A_2:214990_AT Phosphatidylinositol glycan, class O hs
HG-U133_PLUS_2:214990_AT Phosphatidylinositol glycan, class O hs
HG-U133A:214990_AT Phosphatidylinositol glycan, class O hs
HG-U95C:59632_AT Phosphatidylinositol glycan, class O hs
MOUSE430A_2:1438155_X_AT phosphatidylinositol glycan, class O mm
MOE430A:1438155_X_AT phosphatidylinositol glycan, class O mm
MOUSE430_2:1438155_X_AT phosphatidylinositol glycan, class O mm
MG-U74BV2:114076_AT phosphatidylinositol glycan, class O mm
MOE430A:1437142_A_AT phosphatidylinositol glycan, class O mm
MOUSE430A_2:1437142_A_AT phosphatidylinositol glycan, class O mm
MOUSE430_2:1437142_A_AT phosphatidylinositol glycan, class O mm
MOE430A:1415992_AT phosphatidylinositol glycan, class O mm
MOUSE430A_2:1415992_AT phosphatidylinositol glycan, class O mm
MOUSE430_2:1415992_AT phosphatidylinositol glycan, class O mm
MU19KSUBB:TC29271_AT Phosphatidylinositol glycan, class O (Pigo), mRNA mm
RAE230A:1375600_AT Phosphatidylinositol glycan, class O (predicted) rn
RAT230_2:1375600_AT Phosphatidylinositol glycan, class O (predicted) rn
RAE230B:1382724_AT Phosphatidylinositol glycan, class O (predicted) rn
RAT230_2:1382724_AT Phosphatidylinositol glycan, class O (predicted) rn
RAE230B:1384090_AT Phosphatidylinositol glycan, class O (predicted) rn
RAT230_2:1384090_AT Phosphatidylinositol glycan, class O (predicted) rn
RG-U34C:RC_AI176774_AT Phosphatidylinositol glycan, class O (predicted) rn
YEAST_2:1777749_AT ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9117 nucleotide metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB93646 Hypothetical protein C27A12.9 [Caenorhabditis elegans] ref|NP_491750.3| C27A12.9 [Caenorhabditis elegans] 0.0
blast CAE72926 Hypothetical protein CBG20246 [Caenorhabditis briggsae] 1.0E-174
blast AAB93646 Hypothetical protein C27A12.9 [Caenorhabditis elegans] ref|NP_491750.3| C27A12.9 [Caenorhabditis elegans] 1.0E-153
blast BAD90232.1 mFLJ00350 protein [Mus musculus] 1.0E-80
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491750.3 8 7-26,591-613,634-656,698-720,727-749,769-791,812-829,844-863
C27A12.9 8 7-26,591-613,634-656,698-720,727-749,769-791,812-829,844-863
SNAP00000016774 7 31-53,73-95,137-159,166-188,208-230,251-268,283-302
Sequence
Target Sequence 
>C. ELEGANS:184767_S_AT
gtgacgggctgattccggcgataattatattcatagcagtttcagtattaattgctgaat
ttgtgtcaagagaattcattgcatctacaatcgctttgctcatcccactaggattctatc
atcttggacattcaccagttatctcttcaattccatggaatgctgcgtttgttggaattc
ctggtggaacagctcttcgtgtcctccctgcacttttcgtactctttcatctcaactttt
cggcgatctcatcgatttttgtgatctcaagtgaaagtgaatctagtcagaaaatgtctg
cacccaacacagcttggacgttaacagaaactcttgtattgatgacaatacgggcaacgc
tcacttgtttggtctcttcaatacatcggcgtcatctgatggtttggaaaatattcgctc
caaagttcattttcgaatgcattctcaccatcgtcttcattttctcagccaatttctttc
ttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGACGGGCTGATTCCGGCGATAAT 534 481 2183 Antisense
TGCATCTACAATCGCTTTGCTCATC 352 579 2262 Antisense
TGCTCATCCCACTAGGATTCTATCA 262 581 2279 Antisense
GATTCTATCATCTTGGACATTCACC 147 431 2294 Antisense
ATTCCTGGTGGAACAGCTCTTCGTG 197 9 2359 Antisense
TTTCATCTCAACTTTTCGGCGATCT 671 671 2407 Antisense
TGCACCCAACACAGCTTGGACGTTA 367 577 2481 Antisense
GACAATACGGGCAACGCTCACTTGT 165 365 2526 Antisense
GCTCACTTGTTTGGTCTCTTCAATA 690 301 2541 Antisense
TCTCTTCAATACATCGGCGTCATCT 334 607 2555 Antisense
TTTTCTCAGCCAATTTCTTTCTTAT 413 677 2642 Antisense
Sequence Source Affymetrix Proprietary Database