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[184759_at]

Description Y53F4B.12 /REP_DB=WormBase Gene ID /WP=CE22401 /TR=Q9NAB9 /GB=CAB61082.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 25      4grp_high_8_8 > 09      6grp_total > 27      4grp_total > 22      6grp_high1_sub > 01_02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 282
1 2 3 4 range
A 232.3
(3)
P 		89
(-2.3)
P 		70.9
(-3)
A 		231.8
(3)
P 		161
B 288.9
(3)
P 		91.3
(-1.2)
P 		6.6
(-3)
A 		199.8
(1.1)
P 		282
C 233.5
(2)
P 		80.3
(-3)
A 		83.7
(-2.9)
P 		265.5
(3)
P 		185
avr 251.6
(3) 		86.9
(-2) 		53.7
(-3) 		232.4
(2.4) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 176.3
(3)
P 		163.4
(2.4)
P 		64.4
(-2.4)
P 		62.9
(-2.5)
A 		51.6
(-3)
P 		80.5
(-1.6)
P 		125
B 191.1
(3)
P 		158.9
(1.6)
P 		54.4
(-3)
P 		128.3
(0.2)
P 		81.1
(-1.8)
P 		115.4
(-0.3)
P 		137
C 145.2
(1.7)
P 		168.4
(3)
P 		72.4
(-2.3)
P 		60.7
(-3)
A 		98.7
(-0.9)
P 		136.9
(1.2)
M 		108
avr 170.9
(3) 		163.6
(2.6) 		63.7
(-3) 		84
(-1.9) 		77.1
(-2.3) 		110.9
(-0.4) 		107
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 84.6
(3)
P 		19.3
(-2.9)
P 		18.5
(-3)
A 		33.9
(-1.6)
P 		66
B 92.1
(3)
P 		22.6
(-1.6)
P 		1.5
(-3)
A 		42.6
(-0.3)
P 		91
C 77.3
(3)
P 		23.1
(-3)
A 		29.2
(-2.3)
P 		44.8
(-0.6)
P 		54
avr 84.7
(3) 		21.7
(-2.5) 		16.4
(-3) 		40.4
(-0.9) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 32.5
(-0.5)
P 		58.1
(3)
P 		14
(-3)
P 		16
(-2.7)
A 		15.6
(-2.8)
P 		19.6
(-2.2)
P 		44
B 66.5
(3)
P 		52.9
(1.5)
P 		13.2
(-3)
P 		34
(-0.7)
P 		33
(-0.8)
P 		43
(0.4)
P 		53
C 53.8
(3)
P 		51.4
(2.7)
P 		18.4
(-2.1)
P 		12.2
(-3)
A 		31.9
(-0.2)
P 		45.8
(1.8)
M 		42
avr 50.9
(2.5) 		54.1
(3) 		15.2
(-3) 		20.7
(-2.2) 		26.8
(-1.2) 		36.1
(0.2) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184759_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3011
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.12 NCBI
Target Description Y53F4B.12 /REP_DB=WormBase Gene ID /WP=CE22401 /TR=Q9NAB9 /GB=CAB61082.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064696(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064696 NCBI Caenorhabditis elegans Y53F4B.12 (Y53F4B.12) mRNA, complete cds. 11/11 None
SNAP00000043047 ENSEMBL cdna:SNAP chromosome:CEL140:II:15019441:15025585:1 11/11 None
GENEFINDER00000043142 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15019114:15025585:1 11/11 None
Y53F4B.12 ENSEMBL cdna:known chromosome:CEL140:II:15019113:15025658:1 gene:Y53F4B.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15019110-15025582(+) 99.95 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.12
Entrez Gene 190214 Entrez gene
SwissProt Q9NAB9 EMBL-EBI
WormBase CE22401 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB61082 Hypothetical protein Y53F4B.12 [Caenorhabditis elegans] ref|NP_497097.1| Y53F4B.12 [Caenorhabditis elegans] 0.0
blast CAE73540 Hypothetical protein CBG21007 [Caenorhabditis briggsae] 0.0
blast XP_792498 PREDICTED: similar to solute carrier family 7 (cationic amino acid transporter, y+ system), member 4 [Strongylocentrotus purpuratus] 2.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.2E-7
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.2E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497097.1 14 28-50,54-76,96-115,155-177,184-206,221-240,260-282,308-330,351-370,374-396,577-596,601-623,636-658,663-682
Y53F4B.12 14 28-50,54-76,96-115,155-177,184-206,221-240,260-282,308-330,351-370,374-396,577-596,601-623,636-658,663-682
SNAP00000043047 12 7-29,69-91,98-120,135-154,174-196,222-244,265-284,288-310,491-510,515-537,550-572,577-596
GENEFINDER00000043142 14 28-50,54-76,96-115,155-177,184-206,221-240,260-282,308-330,351-370,374-396,577-596,601-623,636-658,663-682
Sequence
Target Sequence 
>C. ELEGANS:184759_AT
agaagcacgcgcacaactgtgtggccgagcagtgtggagctgagaatgatgatgacttca
actcgcaagaggtgcacctattcgcaaacgaggaacccgaattgccattcttctcaacat
tttcctcatcggcaccccgtccaccttcgccagacgtcagtttcgccacgtttcaccgtt
cccggcgtctgctattcctgttttgcgtggtgacttgcgcattttcggcgtttttacgga
actatgcggtgcctacggtgacatgttggatgttaagcatcggatttgtgctggttattg
tggtattcactgtgaatattaaccggctacagactaatgattatttgtacaaaaaagaat
ccaaagtaaccctattcccaaccacctctctcttcacattattcatgctccttggctcat
ttgctggcagtgtgccaacaaaaactttggctcatttggtagtgatacctttaattggaa
tcttggcatatttcctgtatggttaccggcatagcaagcacaggaagactgcttgtttag
tgatagagaatcgatatttggagggtgccgacgagcagtattgtccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGCACGCGCACAACTGTGTGGC 78 75 1553 Antisense
GTGTGGCCGAGCAGTGTGGAGCTGA 547 487 1571 Antisense
GAATTGCCATTCTTCTCAACATTTT 664 323 1651 Antisense
TGCTATTCCTGTTTTGCGTGGTGAC 681 577 1742 Antisense
GCGTGGTGACTTGCGCATTTTCGGC 64 293 1757 Antisense
TTACGGAACTATGCGGTGCCTACGG 107 657 1786 Antisense
GGTGCCTACGGTGACATGTTGGATG 416 501 1800 Antisense
TTCACATTATTCATGCTCCTTGGCT 350 687 1945 Antisense
CCTTGGCTCATTTGCTGGCAGTGTG 412 269 1962 Antisense
GGTTACCGGCATAGCAAGCACAGGA 1 505 2053 Antisense
GGTGCCGACGAGCAGTATTGTCCGA 240 501 2116 Antisense
Sequence Source Affymetrix Proprietary Database