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[184751_at]

Description Y53F4B.19 /REP_DB=WormBase Gene ID /WP=CE25398 /TR=Q9NAA1 /GB=CAB70108.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 10      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 1.6
(-3)
A 		2.3
(2.3)
A 		2.2
(1.5)
A 		2.4
(3)
A 		1
B 2
(-1.7)
A 		2.6
(2.3)
A 		2.7
(3)
A 		1.8
(-3)
A 		1
C 1.8
(-2.8)
A 		2.2
(-2.7)
A 		1.4
(-3)
A 		15.6
(3)
A 		14
avr 1.8
(-3) 		2.4
(-2.3) 		2.1
(-2.6) 		6.6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 2.8
(-2.9)
A 		2.3
(-3)
A 		2
(-3)
A 		3.1
(-2.9)
A 		58.1
(3)
A 		35
(0.5)
A 		56
B 1.9
(-2.9)
A 		1.6
(-3)
A 		3.2
(-2.7)
A 		5.9
(-2.3)
A 		12.7
(-1.1)
A 		37
(3)
A 		35
C 3.6
(-2.8)
A 		1.9
(-3)
A 		2.2
(-3)
A 		14.4
(-1.6)
A 		6.9
(-2.4)
A 		53.7
(3)
A 		52
avr 2.8
(-2.9) 		1.9
(-3) 		2.5
(-2.9) 		7.8
(-2.1) 		25.9
(0.6) 		41.9
(3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 0.6
(3)
A 		0.5
(1)
A 		0.6
(3)
A 		0.3
(-3)
A 		0
B 0.6
(3)
A 		0.6
(3)
A 		0.6
(3)
A 		0.4
(-3)
A 		0
C 0.6
(-2.7)
A 		0.6
(-2.7)
A 		0.5
(-3)
A 		2.6
(3)
A 		2
avr 0.6
(-3) 		0.6
(-3) 		0.6
(-3) 		1.1
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 0.5
(-3)
A 		0.8
(-2.9)
A 		0.4
(-3)
A 		0.8
(-2.9)
A 		17.5
(3)
A 		8.5
(-0.2)
A 		17
B 0.6
(-3)
A 		0.5
(-3)
A 		0.8
(-2.9)
A 		1.6
(-2.5)
A 		5.2
(-0.9)
A 		13.8
(3)
A 		13
C 1.3
(-2.8)
A 		0.6
(-3)
A 		0.6
(-3)
A 		2.9
(-2.2)
A 		2.2
(-2.4)
A 		18
(3)
A 		17
avr 0.8
(-2.9) 		0.6
(-3) 		0.6
(-3) 		1.8
(-2.4) 		8.3
(0.6) 		13.4
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184751_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2828
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.19 NCBI
Target Description Y53F4B.19 /REP_DB=WormBase Gene ID /WP=CE25398 /TR=Q9NAA1 /GB=CAB70108.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064703(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064703 NCBI Caenorhabditis elegans Y53F4B.19 (Y53F4B.19) mRNA, complete cds. 11/11 None
GENEFINDER00000043134 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15077163:15079331:1 11/11 None
Y53F4B.19 ENSEMBL cdna:known chromosome:CEL140:II:15077163:15079331:1 gene:Y53F4B.19 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043053 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15077159-15079328(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.19
Entrez Gene 190218 Entrez gene
SwissProt Q9NAA1 EMBL-EBI
WormBase CE22409 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB70108 Hypothetical protein Y53F4B.19 [Caenorhabditis elegans] ref|NP_497104.2| Y53F4B.19 [Caenorhabditis elegans] 1.0E-171
blast CAB03295 Hypothetical protein T05D4.5 [Caenorhabditis elegans] ref|NP_499807.1| T05D4.5 [Caenorhabditis elegans] 4.0E-9
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 1.6E-9
Sequence
Target Sequence 
>C. ELEGANS:184751_AT
gatgctccgtcgctagtatttatgggccgtcttggcgaagagcccatctctgctcacttc
acacttaccaatcgtacgtgcaagccattctggttcaaaatcaaggtctcgagccgcgaa
cttttcaaagttcgcccaacaaacggaaaattggagaatagtgaaagagtcgtcattaga
gtgattttttacccgaatgtcagagttccgaagccattctgcgagtacttgcacgtttat
gcgttgtttgccgggttgggagatcggcgcgtcctggatagaatgctcaaagcaaacgtt
gttgaaaaggagaaattgttatacatgccaactccaaatctcaacctctacatgcagaac
cttcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCTCCGTCGCTAGTATTTATGG 361 413 193 Antisense
TTATGGGCCGTCTTGGCGAAGAGCC 100 555 212 Antisense
CACTTCACACTTACCAATCGTACGT 499 197 247 Antisense
TCGTACGTGCAAGCCATTCTGGTTC 589 603 264 Antisense
TCTCGAGCCGCGAACTTTTCAAAGT 69 611 299 Antisense
GTCAGAGTTCCGAAGCCATTCTGCG 39 465 391 Antisense
GCCATTCTGCGAGTACTTGCACGTT 589 279 405 Antisense
TTATGCGTTGTTTGCCGGGTTGGGA 135 679 429 Antisense
GATCGGCGCGTCCTGGATAGAATGC 499 415 454 Antisense
ATACATGCCAACTCCAAATCTCAAC 138 25 513 Antisense
CAACCTCTACATGCAGAACCTTCGA 303 187 534 Antisense
Sequence Source Affymetrix Proprietary Database