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[184744_at]

Description F28E10.2 /REP_DB=WormBase Gene ID /WP=CE04459 /TR=Q19882 /GB=AAA81501.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 27      4grp_highest_8_8 > 61      4grp_high_8_8 > 17      6grp_highest_nonorm > 40      6grp_total > 07      4grp_total > 31      6grp_highest_sub > De      6grp_high1_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 700
1 2 3 4 range
A 906.7
(3)
P 		463.1
(-1.4)
P 		305.7
(-3)
P 		661.6
(0.6)
P 		601
B 966
(3)
P 		420.9
(-2.6)
P 		381.2
(-3)
P 		717.8
(0.5)
P 		585
C 1006
(3)
P 		476.8
(-2.1)
P 		379.2
(-3)
P 		642.5
(-0.5)
P 		627
avr 959.6
(3) 		453.6
(-2) 		355.4
(-3) 		674
(0.2) 		604

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 619
1 2 3 4 5 6 range
A 1016.3
(3)
P 		796.2
(0.6)
P 		651.5
(-1)
P 		555.5
(-2)
P 		463.8
(-3)
P 		515.4
(-2.4)
P 		553
B 1006.3
(3)
P 		678.9
(-0.6)
P 		706.2
(-0.3)
P 		459
(-3)
P 		557.9
(-1.9)
P 		548.8
(-2)
P 		547
C 1001.9
(3)
P 		736.3
(0.4)
P 		618.8
(-0.8)
P 		538.7
(-1.6)
P 		397.7
(-3)
P 		622.8
(-0.8)
P 		604
avr 1008.2
(3) 		737.1
(-0) 		658.8
(-0.9) 		517.7
(-2.5) 		473.1
(-3) 		562.3
(-2) 		535
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 253
1 2 3 4 range
A 330.1
(3)
P 		100.5
(-2.5)
P 		79.9
(-3)
P 		96.8
(-2.6)
P 		250
B 308.1
(3)
P 		103.9
(-2.6)
P 		88.3
(-3)
P 		153
(-1.2)
P 		220
C 333
(3)
P 		137.1
(-2.2)
P 		132.2
(-2.4)
P 		108.3
(-3)
P 		225
avr 323.7
(3) 		113.8
(-2.6) 		100.1
(-3) 		119.4
(-2.5) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 263
1 2 3 4 5 6 range
A 187.3
(-0.6)
P 		283.2
(3)
P 		141.9
(-2.4)
P 		140.9
(-2.4)
P 		140
(-2.4)
P 		125.5
(-3)
P 		158
B 350.1
(3)
P 		226
(-0.3)
P 		171.5
(-1.7)
P 		121.6
(-3)
P 		226.7
(-0.2)
P 		204.6
(-0.8)
P 		229
C 371.4
(3)
P 		224.7
(-0.3)
P 		157.5
(-1.9)
P 		108.3
(-3)
P 		128.7
(-2.5)
P 		208.5
(-0.7)
P 		263
avr 302.9
(3) 		244.6
(1) 		157
(-1.9) 		123.6
(-3) 		165.1
(-1.6) 		179.5
(-1.1) 		179
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184744_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11119
Sequence Type Exemplar sequence
Representative Public ID F28E10.2 NCBI
Target Description F28E10.2 /REP_DB=WormBase Gene ID /WP=CE04459 /TR=Q19882 /GB=AAA81501.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068188(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068188 NCBI Caenorhabditis elegans F28E10.2 (F28E10.2) mRNA, complete cds. 11/11 None
SNAP00000006733 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4562658:4565405:1 11/11 None
GENEFINDER00000006743 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4562658:4564882:1 11/11 None
F28E10.2 ENSEMBL cdna:known chromosome:CEL140:IV:4562562:4564882:1 gene:F28E10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4562567-4564888(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28E10.2
Entrez Gene 185065 Entrez gene
SwissProt Q19882 EMBL-EBI
WormBase CE04459 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_500589 F28E10.2 [Caenorhabditis elegans] gb|AAA81501.1| Hypothetical protein F28E10.2 [Caenorhabditis elegans] 1.0E-141
blast NP_500589 F28E10.2 [Caenorhabditis elegans] gb|AAA81501.1| Hypothetical protein F28E10.2 [Caenorhabditis elegans] 1.0E-130
blast NP_500589 F28E10.2 [Caenorhabditis elegans] gb|AAA81501.1| Hypothetical protein F28E10.2 [Caenorhabditis elegans] 1.0E-127
blast CAE58670 Hypothetical protein CBG01840 [Caenorhabditis briggsae] 1.0E-105
blast CAE58670 Hypothetical protein CBG01840 [Caenorhabditis briggsae] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 1.1E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 9.9E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 1.1E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 9.9E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500589.1 1 186-208
F28E10.2 1 186-208
SNAP00000006733 1 178-200
GENEFINDER00000006743 1 178-200
Sequence
Target Sequence 
>C. ELEGANS:184744_AT
acgattaattcattttccctcagtcatacgggtatgtatgaatgtggggcgacaaggaag
aaggatggaaagtatcattcaagacaaatgagagtgaagtctaagagagaaataaacagc
gatctacccccttgctcatcggaatttgacgggatctgtggaaccaatggtatatgcctc
atggatggctccagacagatttgccactgtgacattggatatatgggcgagacatgcgac
aaagtgctcatgggtgcatatgatgtcaaactcctgaaagttgtcggtggaacaactaca
tctctgaaccttgtctgcatcatcatcgccatcctttttgcccttttatacttcaaagaa
cgaaaatcagtgagacggttgcgtcgagaattcgggaaagcagcagaggaatgtgagatg
gaacatgcgatttacaaagagacaacaaccactacaacatctaattcacatgagacaaca
aatgaggggaatacgccaggtttcgccaggaatagcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGATTAATTCATTTTCCCTCAGTC 546 93 289 Antisense
TTTCCCTCAGTCATACGGGTATGTA 602 669 302 Antisense
AGAAATAAACAGCGATCTACCCCCT 634 71 396 Antisense
CCCCTTGCTCATCGGAATTTGACGG 486 261 416 Antisense
GTATATGCCTCATGGATGGCTCCAG 439 453 458 Antisense
GGCTCCAGACAGATTTGCCACTGTG 350 539 475 Antisense
GGAACAACTACATCTCTGAACCTTG 118 529 577 Antisense
GACGGTTGCGTCGAGAATTCGGGAA 517 375 662 Antisense
ACAACCACTACAACATCTAATTCAC 685 111 733 Antisense
TGAGGGGAATACGCCAGGTTTCGCC 217 489 771 Antisense
GCCAGGTTTCGCCAGGAATAGCATT 416 277 783 Antisense
Sequence Source Affymetrix Proprietary Database