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[184735_s_at]

Description C32E12.4 /REP_DB=WormBase Gene ID /WP=CE16888 /TR=P91121 /GB=AAB53880.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 18      6grp_high1_10_10 > 81      4grp_high_8_8 > 29      6grp_highest_nonorm > 81      6grp_total > 81      4grp_total > 26      6grp_highest_sub > 04_05      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 297
1 2 3 4 range
A 193.5
(-3)
P 		311.9
(-0.3)
P 		241.6
(-1.9)
P 		455.5
(3)
P 		262
B 158.7
(-3)
A 		263.7
(-0.1)
P 		179
(-2.4)
P 		377.3
(3)
P 		219
C 186.3
(-3)
P 		386.4
(3)
P 		204
(-2.5)
P 		275.7
(-0.3)
P 		200
avr 179.5
(-3) 		320.7
(1.5) 		208.2
(-2.1) 		369.5
(3) 		190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 269
1 2 3 4 5 6 range
A 322.6
(1.2)
P 		366.5
(2.4)
P 		156.5
(-3)
P 		341.3
(1.7)
P 		391.1
(3)
P 		284.9
(0.3)
P 		235
B 210.6
(-3)
P 		250.7
(-1.2)
P 		320.9
(1.8)
P 		347.3
(3)
P 		304.4
(1.1)
P 		211.9
(-2.9)
P 		137
C 253.5
(-1.2)
P 		241.9
(-1.5)
P 		425.8
(3)
P 		375.5
(1.8)
P 		302.2
(0)
P 		178
(-3)
P 		248
avr 262.2
(-1.3) 		286.4
(-0.2) 		301.1
(0.5) 		354.7
(3) 		332.6
(2) 		224.9
(-3) 		130
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 70.5
(3)
P 		67.7
(0.7)
P 		63.1
(-3)
P 		66.7
(-0.1)
P 		7
B 50.6
(-1.6)
A 		65.1
(0.6)
P 		41.5
(-3)
P 		80.4
(3)
P 		39
C 61.7
(-1.6)
P 		111.1
(3)
P 		71.1
(-0.7)
P 		46.5
(-3)
P 		65
avr 60.9
(-2.4) 		81.3
(3) 		58.6
(-3) 		64.5
(-1.4) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 59.5
(-1.4)
P 		130.4
(3)
P 		34.1
(-3)
P 		86.6
(0.3)
P 		118
(2.2)
P 		69.4
(-0.8)
P 		96
B 73.3
(-3)
P 		83.5
(-1.8)
P 		77.9
(-2.5)
P 		92
(-0.8)
P 		123.7
(3)
P 		79
(-2.3)
P 		50
C 94
(1.2)
P 		73.8
(-1.3)
P 		108.4
(3)
P 		75.5
(-1)
P 		97.8
(1.7)
P 		59.6
(-3)
P 		49
avr 75.6
(-2.1) 		95.9
(0.6) 		73.5
(-2.4) 		84.7
(-0.9) 		113.2
(3) 		69.3
(-3) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184735_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.410
Sequence Type Exemplar sequence
Representative Public ID C32E12.4 NCBI
Target Description C32E12.4 /REP_DB=WormBase Gene ID /WP=CE16888 /TR=P91121 /GB=AAB53880.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059145(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059145 NCBI Caenorhabditis elegans C32E12.4 (C32E12.4) mRNA, complete cds. 11/11 A 
GENEFINDER00000039925 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5230631:5232606:-1 11/11 None
C32E12.4 ENSEMBL cdna:known chromosome:CEL140:I:5230566:5241917:-1 gene:C32E12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5230630-5242486(-) 95.3 100.0
Public Domain and Genome References
Gene Title
Ensembl C32E12.4
Entrez Gene 172163 Entrez gene
SwissProt Q95Q77 EMBL-EBI
WormBase CE30496 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL16309 Hypothetical protein C32E12.4 [Caenorhabditis elegans] ref|NP_491546.2| C32E12.4 [Caenorhabditis elegans] 0.0
blast T25592 hypothetical protein C32E12.4 - Caenorhabditis elegans 0.0
blast CAE67371 Hypothetical protein CBG12847 [Caenorhabditis briggsae] 0.0
blast CAE67371 Hypothetical protein CBG12847 [Caenorhabditis briggsae] 1.0E-102
blast AAL16309 Hypothetical protein C32E12.4 [Caenorhabditis elegans] ref|NP_491546.2| C32E12.4 [Caenorhabditis elegans] 1.0E-91
blast T25592 hypothetical protein C32E12.4 - Caenorhabditis elegans 5.0E-87
Sequence
Target Sequence 
>C. ELEGANS:184735_S_AT
ccggcaagtcttcaaaaccatccacaccgacagcaagaagaaaatctttggcgtcaccaa
ttaccgtcccaaaggatccatcagtgatgtcgacaccggcagctcccggaggaatttcag
ttgttccaaaaagtgaaggagcagctgcaactagaatcagaaatcaggctgctgcttctg
ctggaacgacggtctcatcagttacaacacctgcatcaactgctcttccatcaagtgcaa
ccccatctgtaaccgattcaccaaaagcatcccgtcgttataatgcacgaagaaagactc
aagagattgttaatgagtcgctagtgaaaccacagacaaaaagacgaaagaggaatccga
gaagaagacgatttgtacgagatgatcgagatgctgatctacttctgggatatgagaaaa
atgctacttttgaacagctcgaaaaactgtttgaccaatcatctcgaaatcgtcgcccac
ctgcttcaaaattcgcaaaaattcgtcgattggccccatcaaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGGCAAGTCTTCAAAACCATCCAC 674 265 4187 Antisense
TCTTTGGCGTCACCAATTACCGTCC 400 613 4231 Antisense
ATTACCGTCCCAAAGGATCCATCAG 315 11 4246 Antisense
CATCAGTGATGTCGACACCGGCAGC 63 211 4265 Antisense
GGAACGACGGTCTCATCAGTTACAA 701 529 4369 Antisense
TACAACACCTGCATCAACTGCTCTT 140 643 4389 Antisense
CAACTGCTCTTCCATCAAGTGCAAC 550 187 4403 Antisense
AACCGATTCACCAAAAGCATCCCGT 434 147 4437 Antisense
ACCAATCATCTCGAAATCGTCGCCC 290 85 4640 Antisense
GCCCACCTGCTTCAAAATTCGCAAA 173 287 4661 Antisense
ATTCGTCGATTGGCCCCATCAAAAA 148 3 4687 Antisense
Sequence Source Affymetrix Proprietary Database