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[184728_at]

Description F45E12.1 /REP_DB=WormBase Gene ID /WP=CE02737 /TR=Q20432 /GB=AAA68794.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 0      4grp_high_8_8 > 58      6grp_highest_nonorm > 61      6grp_total > 13      4grp_total > 62      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 263
1 2 3 4 range
A 468.8
(3)
P 		343.5
(-0.4)
P 		386.1
(0.7)
P 		250.1
(-3)
P 		219
B 336.7
(3)
P 		308
(0.9)
P 		301.1
(0.4)
A 		253.8
(-3)
P 		83
C 410.4
(3)
P 		291.9
(-0.5)
M 		366.3
(1.7)
P 		205.4
(-3)
P 		205
avr 405.3
(3) 		314.5
(-0.2) 		351.2
(1.1) 		236.4
(-3) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 343
1 2 3 4 5 6 range
A 459.4
(3)
P 		360.7
(0.3)
P 		399.6
(1.4)
P 		342.8
(-0.1)
P 		384.3
(1)
P 		237
(-3)
P 		222
B 527.9
(3)
P 		464.1
(1.2)
P 		386.3
(-1)
P 		488.8
(1.9)
P 		385.9
(-1)
P 		316.8
(-3)
P 		211
C 452.6
(2.9)
P 		439.1
(2.6)
P 		384
(1.4)
P 		456.5
(3)
P 		293.8
(-0.6)
P 		184.9
(-3)
P 		272
avr 480
(3) 		421.3
(1.5) 		390
(0.7) 		429.4
(1.7) 		354.7
(-0.2) 		246.2
(-3) 		234
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 170.7
(3)
P 		74.6
(-1.3)
P 		100.9
(-0.1)
P 		36.6
(-3)
P 		134
B 107.4
(3)
P 		76.1
(-0.5)
P 		69.8
(-1.2)
A 		54.1
(-3)
P 		53
C 135.9
(3)
P 		83.9
(-0.1)
M 		127.7
(2.5)
P 		34.6
(-3)
P 		101
avr 138
(3) 		78.2
(-0.7) 		99.5
(0.6) 		41.8
(-3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 84.7
(-0.7)
P 		128.3
(3)
P 		87
(-0.5)
P 		86.9
(-0.5)
P 		116
(2)
P 		57.7
(-3)
P 		71
B 183.7
(3)
P 		154.5
(1.1)
P 		93.8
(-3)
P 		129.5
(-0.6)
P 		156.8
(1.2)
P 		118.1
(-1.4)
P 		90
C 167.8
(3)
P 		134
(1.1)
P 		97.7
(-1)
P 		91.8
(-1.3)
P 		95.1
(-1.1)
P 		61.9
(-3)
P 		106
avr 145.4
(3) 		138.9
(2.4) 		92.8
(-1.8) 		102.7
(-0.9) 		122.6
(0.9) 		79.2
(-3) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184728_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4596
Sequence Type Exemplar sequence
Representative Public ID F45E12.1 NCBI
Target Description F45E12.1 /REP_DB=WormBase Gene ID /WP=CE02737 /TR=Q20432 /GB=AAA68794.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063128(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063128 NCBI Caenorhabditis elegans F45E12.1 (F45E12.1) mRNA, complete cds. 11/11 None
SNAP00000037600 ENSEMBL cdna:SNAP chromosome:CEL140:II:7311447:7313187:1 11/11 None
GENEFINDER00000037602 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7311447:7313187:1 11/11 None
F45E12.1 ENSEMBL cdna:known chromosome:CEL140:II:7311447:7313718:1 gene:F45E12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7311443-7313184(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45E12.1
Entrez Gene 185802 Entrez gene
SwissProt Q20432 EMBL-EBI
WormBase CE02737 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1585613_AT similar to dullard homolog cfa
CANINE_2:CFA.11086.1.A1_AT similar to dullard homolog cfa
CANINE_2:CFAAFFX.24712.1.S1_AT similar to dullard homolog cfa
DROSOPHILA_2:1630094_AT dm
DROSGENOME1:145473_AT dm
HG-U133B:200035_AT dullard homolog (Xenopus laevis) hs
PLASMODIUM_ANOPHELES:200035_AT dullard homolog (Xenopus laevis) hs
HG-U133_PLUS_2:200035_AT dullard homolog (Xenopus laevis) hs
HG-U133A:200035_AT dullard homolog (Xenopus laevis) hs
HG-FOCUS:200035_AT dullard homolog (Xenopus laevis) hs
HG-U133A_2:200035_AT dullard homolog (Xenopus laevis) hs
U133_X3P:200035_AT dullard homolog (Xenopus laevis) hs
HU35KSUBD:RC_N70036_AT dullard homolog (Xenopus laevis) hs
HU35KSUBA:C01852_AT Dullard homolog (Xenopus laevis) hs
U133_X3P:G7661721_3P_AT Dullard homolog (Xenopus laevis) hs
HG-U95AV2:38483_AT Dullard homolog (Xenopus laevis) hs
MG-U74BV2:113195_AT Dullard homolog (Xenopus laevis) mm
MOE430A:1452100_AT Dullard homolog (Xenopus laevis) mm
MOUSE430A_2:1452100_AT Dullard homolog (Xenopus laevis) mm
MOUSE430_2:1452100_AT Dullard homolog (Xenopus laevis) mm
MU19KSUBA:TC15621_AT Dullard homolog (Xenopus laevis) (Dullard), mRNA mm
Protein Similarities
Method ID Description E-Value
blast AAA68794 Hypothetical protein F45E12.1 [Caenorhabditis elegans] ref|NP_495529.1| F45E12.1 [Caenorhabditis elegans] 1.0E-130
blast CAE67594 Hypothetical protein CBG13136 [Caenorhabditis briggsae] 1.0E-127
blast AAH85649 Hypothetical protein LOC492343 [Danio rerio] ref|XP_709337.1| PREDICTED: similar to Dullard homolog isoform 2 [Danio rerio] ref|XP_689691.1| PREDICTED: similar to Dullard homolog isoform 1 [Danio rerio] ref|NP_001007310.1| hypothetical protein LOC492343 [Danio rerio] 6.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR004274 EMBL-EBI NLI interacting factor 2.9E-121
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495529.1 1 4-23
F45E12.1 1 4-23
SNAP00000037600 1 4-23
GENEFINDER00000037602 1 4-23
Sequence
Target Sequence 
>C. ELEGANS:184728_AT
tccaatgtctcctcttacaacacatagactattgactgtgaaacggaaaatcctggtttt
ggatcttgacgaaacactcattcattcacatcatgacggtgttcttcgacaaacagtcaa
acctggaactccgtctgattttacgatacgggtggttatcgatcgacatcctgtgaaatt
ctcggttcacgaacgtccacatgtcgactattttttgtcagttgtcagtcaatggtatga
attggtggttttcacagcgagtatggaagtttatggaacatcagtagctgatcggttaga
tcgtggacgaggaattctcaagaggcgttatttccgtcaacattgcacaatggaagtcgg
aggctatacgaaggatttgtctgcaattcatcccgatctttcaagcatctgtattcttga
caactctcctggagcctatcgaaaattcccacacaatgccattccaattccatcatggtt
ttctgatccaaacgacacgtgtcttctcaatcttcttccattcctcgacgcacttcgatt
tacgtcagatgttcgaagtgtgctctcacggaacatgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAATGTCTCCTCTTACAACACAT 198 587 138 Antisense
GACGAAACACTCATTCATTCACATC 657 379 205 Antisense
GTCAAACCTGGAACTCCGTCTGATT 264 465 253 Antisense
GGGTGGTTATCGATCGACATCCTGT 454 497 287 Antisense
GTTCACGAACGTCCACATGTCGACT 431 445 322 Antisense
GGCGTTATTTCCGTCAACATTGCAC 121 541 461 Antisense
TTCATCCCGATCTTTCAAGCATCTG 199 689 524 Antisense
TGACAACTCTCCTGGAGCCTATCGA 547 571 555 Antisense
AAACGACACGTGTCTTCTCAATCTT 435 125 627 Antisense
TTCCTCGACGCACTTCGATTTACGT 141 697 658 Antisense
GAAGTGTGCTCTCACGGAACATGCA 660 335 692 Antisense
Sequence Source Affymetrix Proprietary Database