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[184727_s_at]

Description Y53F4B.15 /REP_DB=WormBase Gene ID /WP=CE28135 /TR=Q9NAA3 /GB=CAB70106.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 65      6grp_total > 56      4grp_total > 03      6grp_cgc > 37      6grp_cgc_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 853
1 2 3 4 range
A 167.3
(-1.7)
M 		112.2
(-2.2)
A 		14.9
(-3)
A 		740.8
(3)
P 		726
B 165.7
(-2.3)
A 		72.4
(-3)
A 		86.6
(-2.9)
A 		867.8
(3)
P 		795
C 111.2
(-2.8)
P 		87.9
(-3)
A 		117.3
(-2.8)
A 		861.6
(3)
P 		774
avr 148.1
(-2.4) 		90.8
(-2.9) 		72.9
(-3) 		823.4
(3) 		751

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 177
1 2 3 4 5 6 range
A 133.3
(-2.1)
A 		156.4
(-1.1)
P 		154.4
(-1.2)
P 		146.2
(-1.6)
P 		110.4
(-3)
P 		259.3
(3)
P 		149
B 160.4
(-1.8)
P 		187.1
(-0.8)
P 		225.6
(0.6)
P 		134.7
(-2.8)
P 		130.3
(-3)
P 		287.1
(3)
P 		157
C 133.2
(-2.3)
P 		208
(3)
P 		123.5
(-3)
M 		199.8
(2.4)
P 		151.5
(-1)
P 		146.6
(-1.4)
P 		85
avr 142.3
(-2.3) 		183.8
(0.2) 		167.8
(-0.8) 		160.2
(-1.2) 		130.7
(-3) 		231
(3) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 60.9
(0.3)
M 		24.3
(-1.8)
A 		3.9
(-3)
A 		108.4
(3)
P 		105
B 52.9
(-1.7)
A 		17.9
(-3)
A 		20.1
(-2.9)
A 		184.9
(3)
P 		167
C 36.8
(-2.4)
P 		25.3
(-3)
A 		40.9
(-2.2)
A 		145.3
(3)
P 		120
avr 50.2
(-1.6) 		22.5
(-3) 		21.6
(-3) 		146.2
(3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 24.6
(-3)
A 		55.6
(1.8)
P 		33.6
(-1.6)
P 		37.1
(-1.1)
P 		33.3
(-1.6)
P 		63.1
(3)
P 		39
B 55.8
(-1.3)
P 		62.3
(-0.8)
P 		54.8
(-1.4)
P 		35.7
(-3)
P 		52.9
(-1.6)
P 		107.1
(3)
P 		71
C 49.4
(0.4)
P 		63.5
(3)
P 		31.4
(-3)
M 		40.2
(-1.4)
P 		49
(0.3)
P 		49.1
(0.3)
P 		32
avr 43.3
(-2.1) 		60.5
(0.9) 		39.9
(-2.6) 		37.7
(-3) 		45.1
(-1.7) 		73.1
(3) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184727_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3429
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.15 NCBI
Target Description Y53F4B.15 /REP_DB=WormBase Gene ID /WP=CE28135 /TR=Q9NAA3 /GB=CAB70106.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064699(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064699 NCBI Caenorhabditis elegans human Activating Signal Cointegrator homolog family member (asc-1) (asc-1) mRNA, complete cds. 11/11 None
SNAP00000043050 ENSEMBL cdna:SNAP chromosome:CEL140:II:15043882:15048745:1 11/11 None
GENEFINDER00000043153 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15039306:15051165:1 10/11 None
Y53F4B.15 ENSEMBL cdna:known chromosome:CEL140:II:15039293:15048794:1 gene:Y53F4B.15 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15039302-15051162(+) 98.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol asc-1
Ensembl Y53F4B.15
Entrez Gene 175159 Entrez gene
SwissProt Q9NAA3 EMBL-EBI
WormBase CE28135 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.19738.1.S1_S_AT similar to thyroid hormone receptor interactor 4 cfa
CANINE_2:CFAAFFX.26150.1.S1_AT similar to thyroid hormone receptor interactor 4 cfa
CANINE_2:CFAAFFX.26151.1.S1_S_AT similar to thyroid hormone receptor interactor 4 cfa
DROSOPHILA_2:1640187_A_AT dm
DROSGENOME1:145428_AT dm
CHICKEN:GGA.16312.1.S1_AT thyroid hormone receptor interactor 4 gga
HG-U133_PLUS_2:203732_AT thyroid hormone receptor interactor 4 hs
HG-U133A:203732_AT thyroid hormone receptor interactor 4 hs
HG-FOCUS:203732_AT thyroid hormone receptor interactor 4 hs
HG-U133A_2:203732_AT thyroid hormone receptor interactor 4 hs
HUGENEFL:L40371_AT thyroid hormone receptor interactor 4 hs
U133_X3P:G7706430_3P_AT thyroid hormone receptor interactor 4 hs
HG-U95AV2:41611_AT thyroid hormone receptor interactor 4 hs
HG-U95B:43599_AT Thyroid hormone receptor interactor 4 hs
HU35KSUBC:RC_T65972_AT Thyroid hormone receptor interactor 4 hs
MOE430A:1450785_AT thyroid hormone receptor interactor 4 mm
MOUSE430A_2:1450785_AT thyroid hormone receptor interactor 4 mm
MOUSE430_2:1450785_AT thyroid hormone receptor interactor 4 mm
MG-U74BV2:107589_AT thyroid hormone receptor interactor 4 mm
MOE430A:1422858_AT thyroid hormone receptor interactor 4 mm
MOUSE430A_2:1422858_AT thyroid hormone receptor interactor 4 mm
MOUSE430_2:1422858_AT thyroid hormone receptor interactor 4 mm
MOE430A:1422857_AT thyroid hormone receptor interactor 4 mm
MOUSE430A_2:1422857_AT thyroid hormone receptor interactor 4 mm
MOUSE430_2:1422857_AT thyroid hormone receptor interactor 4 mm
RG-U34C:RC_AI172553_AT thyroid hormone receptor interactor 4 (predicted) rn
RAE230B:1382288_AT thyroid hormone receptor interactor 4 (predicted) rn
RAT230_2:1382288_AT thyroid hormone receptor interactor 4 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
40024 dauer larval development inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16563 transcriptional activator activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB70106 Hypothetical protein Y53F4B.15 [Caenorhabditis elegans] ref|NP_497100.1| human Activating Signal Cointegrator homolog family member (asc-1) [Caenorhabditis elegans] gb|AAF18441.1| ASC-1 [Caenorhabditis elegans] 0.0
blast CAE73459 Hypothetical protein CBG20909 [Caenorhabditis briggsae] 0.0
blast CAE73459 Hypothetical protein CBG20909 [Caenorhabditis briggsae] 1.0E-180
blast CAB70106 Hypothetical protein Y53F4B.15 [Caenorhabditis elegans] ref|NP_497100.1| human Activating Signal Cointegrator homolog family member (asc-1) [Caenorhabditis elegans] gb|AAF18441.1| ASC-1 [Caenorhabditis elegans] 1.0E-101
blast CAE73459 Hypothetical protein CBG20909 [Caenorhabditis briggsae] 7.0E-82
blast XP_413717 PREDICTED: similar to thyroid hormone receptor interactor 4; thyroid receptor interacting protein 4; activating signal cointegrator 1 [Gallus gallus] 1.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR009349 EMBL-EBI Putative zinc finger, C2HC5-type 2.8E-35
Sequence
Target Sequence 
>C. ELEGANS:184727_S_AT
gacagaagacggcgatcatccacaacaagaatgcagtgacggagatattatagacgcaga
tagtcttatgattgttagtgatgagctcgctgcacaaatcaccgaagaaaagggatacgt
acttgcacttgctcagccgacagcgagttttcttgtcaatggactgtgcagatttattcg
atggccgactgatttgaatctcaagggaccactatttattactgccaaggcgggcggtac
gaacgagaaaatgattcgcgaatttgcgaagaaatatgttcgagacgtgaataagatcag
cgagctggatttctccgacggagccgtgctgggaagagcatttctacaggaatgtatgat
gctcgaggatttcctcgacaagtatggtagcaagtcggcgattcccggcggcgacggaca
atttgttttgtgtttctccgcgttcgagccgctggtttcaacgattccacacatttccac
cgctgattcgtatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAGAAGACGGCGATCATCCACAA 705 367 777 Antisense
AGCTCGCTGCACAAATCACCGAAGA 689 79 860 Antisense
AAGGGATACGTACTTGCACTTGCTC 264 159 886 Antisense
TGCTCAGCCGACAGCGAGTTTTCTT 262 579 906 Antisense
ATTACTGCCAAGGCGGGCGGTACGA 481 13 994 Antisense
GATCAGCGAGCTGGATTTCTCCGAC 705 419 1071 Antisense
TTCTCCGACGGAGCCGTGCTGGGAA 8 681 1087 Antisense
TGGTAGCAAGTCGGCGATTCCCGGC 51 553 1161 Antisense
TTCCCGGCGGCGACGGACAATTTGT 334 697 1178 Antisense
TTTCAACGATTCCACACATTTCCAC 1 671 1232 Antisense
ACATTTCCACCGCTGATTCGTATAT 167 105 1247 Antisense
Sequence Source Affymetrix Proprietary Database