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[184725_at]

Description F28E10.1 /REP_DB=WormBase Gene ID /WP=CE28224 /TR=Q19884 /GB=AAA81503.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 15      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 60      6grp_total > 15      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 269
1 2 3 4 range
A 515.1
(1.6)
P 		366.3
(-3)
P 		374.9
(-2.7)
P 		561.4
(3)
P 		195
B 578.2
(3)
P 		325.8
(-2.6)
P 		308.9
(-3)
P 		405.1
(-0.9)
P 		269
C 538.7
(3)
P 		369.9
(-1.4)
P 		311.1
(-3)
P 		408.1
(-0.4)
P 		228
avr 544
(3) 		354
(-2.4) 		331.6
(-3) 		458.2
(0.6) 		212

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 459
1 2 3 4 5 6 range
A 566.6
(2.4)
P 		597.9
(3)
P 		319.9
(-2.2)
P 		385.2
(-1)
P 		277.6
(-3)
P 		300.4
(-2.6)
P 		320
B 514.2
(3)
P 		438.3
(1.6)
P 		283.7
(-1.2)
P 		264.6
(-1.6)
P 		187.3
(-3)
P 		195.8
(-2.8)
P 		327
C 566.4
(3)
P 		394.7
(0.6)
P 		369.8
(0.2)
P 		265.6
(-1.2)
P 		306.8
(-0.6)
P 		138.7
(-3)
P 		428
avr 549.1
(3) 		477
(1.7) 		324.5
(-1) 		305.1
(-1.3) 		257.2
(-2.2) 		211.6
(-3) 		338
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 187.5
(3)
P 		79.5
(-3)
P 		98
(-2)
P 		82.2
(-2.9)
P 		108
B 184.4
(3)
P 		80.5
(-2.5)
P 		71.6
(-3)
P 		86.3
(-2.2)
P 		113
C 178.3
(3)
P 		106.4
(-0.9)
P 		108.4
(-0.8)
P 		68.8
(-3)
P 		110
avr 183.4
(3) 		88.8
(-2.4) 		92.7
(-2.2) 		79.1
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 166
1 2 3 4 5 6 range
A 104.4
(-1.5)
P 		212.6
(3)
P 		69.7
(-3)
P 		97.7
(-1.8)
P 		83.8
(-2.4)
P 		73.1
(-2.9)
P 		143
B 178.9
(3)
P 		145.9
(1.2)
P 		68.9
(-3)
P 		70.1
(-2.9)
P 		76.1
(-2.6)
P 		73
(-2.8)
P 		110
C 209.9
(3)
P 		120.4
(-0.3)
P 		94.1
(-1.2)
P 		53.4
(-2.7)
P 		99.3
(-1.1)
P 		46.4
(-3)
P 		164
avr 164.4
(3) 		159.6
(2.7) 		77.6
(-2.2) 		73.7
(-2.4) 		86.4
(-1.7) 		64.2
(-3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184725_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11192
Sequence Type Exemplar sequence
Representative Public ID F28E10.1 NCBI
Target Description F28E10.1 /REP_DB=WormBase Gene ID /WP=CE28224 /TR=Q19884 /GB=AAA81503.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027981(11), NM_001027984(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027981 NCBI Caenorhabditis elegans F28E10.1a (F28E10.1) mRNA, complete cds. 11/11 None
NM_001027984 NCBI Caenorhabditis elegans F28E10.1d (F28E10.1) mRNA, complete cds. 11/11 None
GENEFINDER00000006740 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4568834:4590062:1 11/11 None
F28E10.1a ENSEMBL cdna:known chromosome:CEL140:IV:4572236:4590281:1 gene:F28E10.1 11/11 None
F28E10.1d ENSEMBL cdna:known chromosome:CEL140:IV:4583137:4590062:1 gene:F28E10.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027983 3/11 Cross Hyb Matching Probes None
NM_001027982 3/11 Cross Hyb Matching Probes None
SNAP00000006737 5/11 Cross Hyb Matching Probes None
F28E10.1c 3/11 Cross Hyb Matching Probes None
F28E10.1b 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177223 4 2 NM_001027981 184725_at (11)
NM_001027984 184725_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4578312-4590068(+) 100.0 100.0
Public Domain and Genome References
Ensembl F28E10.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023152 F28E10.1a [Caenorhabditis elegans] gb|AAV34792.1| Hypothetical protein F28E10.1a [Caenorhabditis elegans] 0.0
blast T16203 hypothetical protein F28E10.1 - Caenorhabditis elegans 0.0
blast NP_001023154 F28E10.1c [Caenorhabditis elegans] gb|AAO91728.1| Hypothetical protein F28E10.1c [Caenorhabditis elegans] 0.0
blast NP_001023155 F28E10.1d [Caenorhabditis elegans] gb|AAO91729.1| Hypothetical protein F28E10.1d [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:184725_AT
ctcacaccgtcgtttttgcggaaggaaaagaagggccccaagaagtcactctacgcaaaa
accacatcatccggccgccaacacatttctgaaaagaaagaaaagaaggcccctctagcc
gaaggagaatccacagtggttgccaactctccgctcgtcgagaatcttccaattacattc
gaaaaatggagcaataagagcccacgtctgagtgtgaaacacttcaacgagaccatcatt
gaagagacactggatgcgaatgcaaattccatgtcgccacagttggagcatcgcgttatt
ccaacgtcgcatcatgtccaagacaacaccggactcattgacgatgacattctaaggagc
agcaatcatgcggcggctcaaaataatgcgaacaggaacacccaatacatcgaaacgtcg
ttggatcggcaaatcgacgctggccgttccgaagttcaggacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCACACCGTCGTTTTTGCGGAAGG 485 225 2527 Antisense
AAGAAGGGCCCCAAGAAGTCACTCT 122 151 2554 Antisense
GGCCGCCAACACATTTCTGAAAAGA 407 541 2599 Antisense
GAATCCACAGTGGTTGCCAACTCTC 335 329 2653 Antisense
TCCGCTCGTCGAGAATCTTCCAATT 548 595 2676 Antisense
GCAAATTCCATGTCGCCACAGTTGG 622 317 2788 Antisense
GTTATTCCAACGTCGCATCATGTCC 4 449 2821 Antisense
AGACAACACCGGACTCATTGACGAT 602 71 2847 Antisense
AAGGAGCAGCAATCATGCGGCGGCT 347 161 2880 Antisense
TCGGCAAATCGACGCTGGCCGTTCC 655 605 2952 Antisense
CTGGCCGTTCCGAAGTTCAGGACAT 565 241 2966 Antisense
Sequence Source Affymetrix Proprietary Database