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[184714_s_at]

Description Y53G8B.2 /REP_DB=WormBase Gene ID /WP=CE25428 /TR=Q9N4S3 /GB=AAF60755.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 15      4grp_high_8_8 > 06      6grp_highest_nonorm > 99      6grp_total > 67      4grp_total > 02      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4380
1 2 3 4 range
A 1014
(-3)
P 		1173
(-2.8)
P 		1153.2
(-2.8)
P 		5393.5
(3)
P 		4380
B 1071.7
(-3)
P 		1194
(-2.8)
P 		1227
(-2.7)
P 		4243.7
(3)
P 		3172
C 1056.1
(-2.9)
P 		1442.4
(-2.3)
P 		1019.2
(-3)
P 		4863.4
(3)
P 		3844
avr 1047.3
(-3) 		1269.8
(-2.6) 		1133.1
(-2.9) 		4833.5
(3) 		3786

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4542
1 2 3 4 5 6 range
A 1058.1
(-2.1)
P 		385.1
(-3)
P 		960.1
(-2.2)
P 		959.1
(-2.2)
P 		859.2
(-2.4)
P 		4927.4
(3)
P 		4542
B 784.1
(-2.5)
P 		477.3
(-3)
P 		818.2
(-2.4)
P 		1188.9
(-1.8)
P 		850.4
(-2.4)
P 		3962.1
(3)
P 		3485
C 746
(-2.6)
P 		528.1
(-3)
P 		935.4
(-2.3)
P 		1103
(-2)
P 		789
(-2.6)
P 		4053.2
(3)
P 		3525
avr 862.7
(-2.4) 		463.5
(-3) 		904.6
(-2.3) 		1083.7
(-2) 		832.9
(-2.4) 		4314.2
(3) 		3851
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 650
1 2 3 4 range
A 369.2
(-1.7)
P 		254.6
(-3)
P 		301.5
(-2.5)
P 		789.5
(3)
P 		535
B 341.8
(-2.4)
P 		294.9
(-2.9)
P 		284.3
(-3)
P 		904.3
(3)
P 		620
C 349.6
(-3)
P 		414.7
(-2.2)
P 		355.2
(-2.9)
P 		820
(3)
P 		470
avr 353.5
(-2.5) 		321.4
(-2.9) 		313.7
(-3) 		837.9
(3) 		524

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1340
1 2 3 4 5 6 range
A 195
(-2.7)
P 		137
(-3)
P 		209.1
(-2.6)
P 		243.2
(-2.4)
P 		259.3
(-2.3)
P 		1199.7
(3)
P 		1063
B 272.8
(-2.5)
P 		158.9
(-3)
P 		198.7
(-2.8)
P 		314.9
(-2.3)
P 		345.5
(-2.2)
P 		1477.4
(3)
P 		1319
C 276.5
(-2.4)
P 		161.1
(-3)
P 		238.1
(-2.6)
P 		221.8
(-2.7)
P 		255.3
(-2.5)
P 		1356.8
(3)
P 		1196
avr 248.1
(-2.5) 		152.3
(-3) 		215.3
(-2.7) 		260
(-2.5) 		286.7
(-2.3) 		1344.6
(3) 		1192
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184714_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7183
Sequence Type Exemplar sequence
Representative Public ID Y53G8B.2 NCBI
Target Description Y53G8B.2 /REP_DB=WormBase Gene ID /WP=CE25428 /TR=Q9N4S3 /GB=AAF60755.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065258(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065258 NCBI Caenorhabditis elegans Y53G8B.2 (Y53G8B.2) mRNA, complete cds. 11/11 None
SNAP00000030865 ENSEMBL cdna:SNAP chromosome:CEL140:III:3101392:3102585:-1 11/11 None
GENEFINDER00000030872 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3101392:3103928:-1 11/11 None
Y53G8B.2 ENSEMBL cdna:known chromosome:CEL140:III:3101283:3104237:-1 gene:Y53G8B.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3101391-3103928(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8B.2
Entrez Gene 175417 Entrez gene
SwissProt Q9N4S3 EMBL-EBI
WormBase CE25428 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1592973_AT similar to diacylglycerol O-acyltransferase 2 cfa
CANINE_2:CFA.14378.1.S1_AT similar to diacylglycerol O-acyltransferase 2 cfa
CANINE_2:CFA.21170.1.S1_AT similar to diacylglycerol O-acyltransferase 2 cfa
CANINE_2:CFA.21170.1.S1_S_AT similar to diacylglycerol O-acyltransferase 2 cfa
CANINE_2:CFAAFFX.8977.1.S1_S_AT similar to diacylglycerol O-acyltransferase 2 cfa
CHICKEN:GGAAFFX.22808.1.S1_AT similar to diacylglycerol O-acyltransferase homolog 2; GS1999full gga
HG-U133_PLUS_2:224327_S_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
HG-U133B:224327_S_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
HU35KSUBA:RC_AA621131_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
HU35KSUBC:RC_W58756_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
U133_X3P:G13537296_3P_S_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
U133_X3P:HS.74637.1.A1_3P_S_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
HG-U133_PLUS_2:226064_S_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
HG-U133B:226064_S_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
HG-U95B:53200_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
HG-U95C:61308_AT diacylglycerol O-acyltransferase homolog 2 (mouse) hs
MU11KSUBB:W15862_S_AT diacylglycerol O-acyltransferase 2 mm
MOE430A:1422677_AT diacylglycerol O-acyltransferase 2 mm
MOUSE430A_2:1422677_AT diacylglycerol O-acyltransferase 2 mm
MOUSE430_2:1422677_AT diacylglycerol O-acyltransferase 2 mm
MOE430A:1422678_AT diacylglycerol O-acyltransferase 2 mm
MOUSE430A_2:1422678_AT diacylglycerol O-acyltransferase 2 mm
MOUSE430_2:1422678_AT diacylglycerol O-acyltransferase 2 mm
MU19KSUBC:TC35994_AT Diacylglycerol O-acyltransferase 2 (Dgat2), mRNA mm
MU19KSUBC:TC35994_G_AT Diacylglycerol O-acyltransferase 2 (Dgat2), mRNA mm
MU19KSUBC:TC35995_I_AT Diacylglycerol O-acyltransferase 2 (Dgat2), mRNA mm
RG-U34B:RC_AI012505_AT diacylglycerol O-acyltransferase homolog 2 (mouse) rn
RG-U34B:RC_AI045040_AT diacylglycerol O-acyltransferase homolog 2 (mouse) rn
RAE230A:1371615_AT diacylglycerol O-acyltransferase homolog 2 (mouse) rn
RAT230_2:1371615_AT diacylglycerol O-acyltransferase homolog 2 (mouse) rn
RAE230B:1391045_AT diacylglycerol O-acyltransferase homolog 2 (mouse) rn
RAT230_2:1391045_AT diacylglycerol O-acyltransferase homolog 2 (mouse) rn
Protein Similarities
Method ID Description E-Value
blast AAF60755 Hypothetical protein Y53G8B.2 [Caenorhabditis elegans] ref|NP_497659.1| Y53G8B.2 [Caenorhabditis elegans] 0.0
blast CAE74669 Hypothetical protein CBG22470 [Caenorhabditis briggsae] 1.0E-180
blast CAE74670 Hypothetical protein CBG22471 [Caenorhabditis briggsae] 1.0E-126
blast AAF60755 Hypothetical protein Y53G8B.2 [Caenorhabditis elegans] ref|NP_497659.1| Y53G8B.2 [Caenorhabditis elegans] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR007130 EMBL-EBI Diacylglycerol acyltransferase 1.0E-126
Pfam IPR007130 EMBL-EBI Diacylglycerol acyltransferase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497659.1 1 25-47
Y53G8B.2 1 25-47
GENEFINDER00000030872 1 25-47
Sequence
Target Sequence 
>C. ELEGANS:184714_S_AT
tttagatgctcatccaggctaccacacactcactttggcatcaagaaagggatttgttcg
ggaggctttgattactggtgcctacttggtcccggtgtattcttttggagagaatgacgt
ttttgaacagatggagaatccagttggctcacggctccgcaacttccaagaatggtgcaa
aagcattttcggcatctcatatccaattttccatggtcgtggcttcttccaactaacttt
cggatatcttccattccgtaaaccaatcgataccgtagtcggagccccaattcccgttga
aaaagtggaaaatccgacgaaagagcaaatcgacgagcttcacaccatttattgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTAGATGCTCATCCAGGCTACCAC 523 673 603 Antisense
CTACCACACACTCACTTTGGCATCA 499 225 621 Antisense
TTGATTACTGGTGCCTACTTGGTCC 433 673 670 Antisense
TTGGTCCCGGTGTATTCTTTTGGAG 507 705 688 Antisense
TGGAGAATCCAGTTGGCTCACGGCT 329 549 734 Antisense
TTTCGGCATCTCATATCCAATTTTC 318 671 789 Antisense
AATTTTCCATGGTCGTGGCTTCTTC 357 175 807 Antisense
TGGCTTCTTCCAACTAACTTTCGGA 358 543 822 Antisense
ATATCTTCCATTCCGTAAACCAATC 14 15 846 Antisense
AATCGATACCGTAGTCGGAGCCCCA 622 167 867 Antisense
GACGAGCTTCACACCATTTATTGTC 555 379 934 Antisense
Sequence Source Affymetrix Proprietary Database