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[184712_s_at]

Description H38K22.2A /REP_DB=WormBase Gene ID /WP=CE23836 /TR=Q9U3C8 /GB=CAB54261.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 91      6grp_high1_10_10 > 59      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 73      6grp_total > 39      4grp_total > 62      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 651.5
(3)
P 		588.9
(-2.6)
P 		624
(0.5)
P 		584.4
(-3)
P 		67
B 610.7
(3)
P 		529.3
(-2.4)
P 		571.4
(0.4)
P 		520
(-3)
P 		91
C 613.1
(2.7)
P 		615.3
(2.8)
P 		620.6
(3)
P 		490.5
(-3)
P 		130
avr 625.1
(3) 		577.8
(-0) 		605.3
(1.7) 		531.6
(-3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 284
1 2 3 4 5 6 range
A 527.2
(0.4)
P 		488.6
(-0.6)
P 		402.6
(-3)
P 		429.1
(-2.3)
P 		622.1
(3)
P 		568
(1.5)
P 		220
B 618
(2.7)
P 		576.7
(1.5)
P 		417.1
(-3)
P 		480
(-1.2)
P 		627.7
(3)
P 		598.5
(2.2)
P 		211
C 521.8
(1.3)
P 		440.2
(-0.7)
P 		478.4
(0.3)
P 		343.7
(-3)
P 		592.1
(3)
P 		526.1
(1.4)
P 		248
avr 555.7
(1.2) 		501.8
(-0.4) 		432.7
(-2.5) 		417.6
(-3) 		614
(3) 		564.2
(1.5) 		196
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 237.2
(3)
P 		127.8
(-1.3)
P 		163.1
(0.1)
P 		85.5
(-3)
P 		152
B 194.8
(3)
P 		130.7
(-1.6)
P 		132.4
(-1.5)
P 		110.8
(-3)
P 		84
C 202.9
(2.4)
P 		176.9
(1.2)
P 		216.3
(3)
P 		82.7
(-3)
P 		134
avr 211.6
(3) 		145.1
(-0.4) 		170.6
(0.9) 		93
(-3) 		119

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 97.2
(-2.4)
P 		173.8
(2.2)
P 		87.7
(-3)
P 		108.8
(-1.7)
P 		187.7
(3)
P 		138.3
(0)
P 		100
B 215
(1.4)
P 		192
(0.5)
P 		101.3
(-3)
P 		127.1
(-2)
P 		255
(3)
P 		223.2
(1.8)
P 		154
C 193.4
(3)
P 		134.3
(0.1)
P 		121.8
(-0.5)
P 		69.1
(-3)
P 		191.6
(2.9)
P 		176.1
(2.2)
P 		124
avr 168.5
(0.7) 		166.7
(0.6) 		103.6
(-2.9) 		101.7
(-3) 		211.4
(3) 		179.2
(1.2) 		110
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184712_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6550
Sequence Type Exemplar sequence
Representative Public ID H38K22.2A NCBI
Target Description H38K22.2A /REP_DB=WormBase Gene ID /WP=CE23836 /TR=Q9U3C8 /GB=CAB54261.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065466(11), NM_065465(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065466 NCBI Caenorhabditis elegans Defective in Cullin Neddylation family member (dcn-1) (dcn-1) mRNA, complete cds. 11/11 None
NM_065465 NCBI Caenorhabditis elegans Defective in Cullin Neddylation family member (dcn-1) (dcn-1) mRNA, complete cds. 10/11 None
SNAP00000023311 ENSEMBL cdna:SNAP chromosome:CEL140:III:4312498:4316447:1 10/11 None
GENEFINDER00000023316 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4313918:4317021:1 11/11 None
H38K22.2b ENSEMBL cdna:known chromosome:CEL140:III:4313593:4317547:1 gene:H38K22.2 11/11 A 
H38K22.2a ENSEMBL cdna:known chromosome:CEL140:III:4313897:4316447:1 gene:H38K22.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4313917-4317021(+) 100.0 100.0
Public Domain and Genome References
Ensembl H38K22.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast G88424 protein H38K22.2 [imported] - Caenorhabditis elegans 0.0
blast CAB54261 Hypothetical protein H38K22.2a [Caenorhabditis elegans] ref|NP_497866.2| Defective in Cullin Neddylation family member (dcn-1) [Caenorhabditis elegans] sp|Q9U3C8|DCN1_CAEEL Defective in cullin neddylation protein 1.0E-174
blast CAB54261 Hypothetical protein H38K22.2a [Caenorhabditis elegans] ref|NP_497866.2| Defective in Cullin Neddylation family member (dcn-1) [Caenorhabditis elegans] sp|Q9U3C8|DCN1_CAEEL Defective in cullin neddylation protein 1.0E-168
blast G88424 protein H38K22.2 [imported] - Caenorhabditis elegans 1.0E-166
blast CAB54261 Hypothetical protein H38K22.2a [Caenorhabditis elegans] ref|NP_497866.2| Defective in Cullin Neddylation family member (dcn-1) [Caenorhabditis elegans] sp|Q9U3C8|DCN1_CAEEL Defective in cullin neddylation protein 1.0E-166
blast CAB54260 Hypothetical protein H38K22.2b [Caenorhabditis elegans] ref|NP_497867.2| Defective in Cullin Neddylation family member (dcn-1) [Caenorhabditis elegans] 1.0E-142
Protein Domains
Database ID Description E-Value
Pfam IPR005176 EMBL-EBI Protein of unknown function DUF298 3.1E-67
Pfam IPR005176 EMBL-EBI Protein of unknown function DUF298 3.1E-67
Pfam IPR005176 EMBL-EBI Protein of unknown function DUF298 3.1E-67
Pfam IPR005176 EMBL-EBI Protein of unknown function DUF298 3.1E-67
Sequence
Target Sequence 
>C. ELEGANS:184712_S_AT
aaggcaaaattccacgagctctacctatttgccttcaactatgccaaatccgccgcttgc
cgcaatctggatcttgaaactgccatctgttgctgggatgttcttttcggacaacgatca
acaattatgactcaatggatcgattttctatgggcacaggagaacgcggcggcgtctcgc
ctcgctcagaacgtgggcgcttccaatgcgaagcaattcaaatcggtgtggatctctcgt
gacacgtggaatctcttctgggactttattcttctgagtaagccagatttgtcggattac
gatgatgaaggagcatggccagtgcttattgatcaattcgttgattattgccgtgaaaat
ctcaattatccaaagccaggaaatgcgtcaaatgatcagcaaatggagacaccaaaaata
gcgcaaaagaaacccggaattttttattttaattctaatttacaactaatagaattcaaa
ttgtttcagtatcccatgctcaagactatcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGCAAAATTCCACGAGCTCTACC 288 161 475 Antisense
TGCCTTCAACTATGCCAAATCCGCC 40 585 504 Antisense
GCTTGCCGCAATCTGGATCTTGAAA 367 307 529 Antisense
GATCTTGAAACTGCCATCTGTTGCT 110 421 544 Antisense
TGTTGCTGGGATGTTCTTTTCGGAC 435 561 562 Antisense
TATGGGCACAGGAGAACGCGGCGGC 12 657 623 Antisense
TCAGAACGTGGGCGCTTCCAATGCG 138 623 660 Antisense
CAAATCGGTGTGGATCTCTCGTGAC 462 193 693 Antisense
GTGACACGTGGAATCTCTTCTGGGA 86 483 713 Antisense
TCTTCTGGGACTTTATTCTTCTGAG 283 617 728 Antisense
GTATCCCATGCTCAAGACTATCTTC 121 455 963 Antisense
Sequence Source Affymetrix Proprietary Database