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[184688_at]

Description Y49F6B.8 /REP_DB=WormBase Gene ID /WP=CE25341 /TR=Q9N4U6 /GB=AAF60734.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 89      4grp_total > 37      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 103.7
(3)
A 		78
(0.1)
A 		78.8
(0.1)
A 		51.4
(-3)
A 		52
B 105
(3)
A 		72.5
(0.4)
A 		29.2
(-3)
A 		99.5
(2.6)
A 		76
C 91.1
(-1)
A 		136.9
(3)
A 		81.4
(-1.8)
A 		67.5
(-3)
A 		69
avr 99.9
(3) 		95.8
(2.3) 		63.1
(-3) 		72.8
(-1.4) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 93.6
(-2)
A 		72
(-2.8)
A 		69.8
(-2.9)
A 		67.3
(-3)
A 		227.5
(3)
P 		203.3
(2.1)
P 		160
B 81
(-2.1)
A 		78.6
(-2.3)
A 		62.6
(-3)
A 		66.7
(-2.8)
A 		141.5
(0.7)
P 		191.2
(3)
P 		129
C 77.6
(-1.7)
A 		108.4
(-0.6)
A 		38.6
(-3)
A 		89.6
(-1.3)
A 		154.8
(1)
P 		214
(3)
P 		175
avr 84.1
(-1.9) 		86.3
(-1.8) 		57
(-3) 		74.5
(-2.3) 		174.6
(1.8) 		202.8
(3) 		146
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 37.8
(3)
A 		16.9
(-1.1)
A 		20.6
(-0.4)
A 		7.5
(-3)
A 		30
B 33.5
(3)
A 		17.9
(-0.5)
A 		6.8
(-3)
A 		21.2
(0.2)
A 		27
C 30.2
(1)
A 		39.4
(3)
A 		28.4
(0.6)
A 		11.4
(-3)
A 		28
avr 33.8
(3) 		24.7
(0.3) 		18.6
(-1.5) 		13.4
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 17.2
(-2.8)
A 		25.6
(-1.8)
A 		15.2
(-3)
A 		17.1
(-2.8)
A 		68.7
(3)
P 		49.5
(0.8)
P 		54
B 28.2
(-1.6)
A 		26.2
(-1.8)
A 		15.2
(-3)
A 		17.7
(-2.7)
A 		57.5
(1.5)
P 		71.3
(3)
P 		56
C 28.8
(-1.2)
A 		33.1
(-0.7)
A 		9.8
(-3)
A 		18
(-2.2)
A 		50.1
(0.9)
P 		71.7
(3)
P 		62
avr 24.7
(-1.7) 		28.3
(-1.2) 		13.4
(-3) 		17.6
(-2.5) 		58.8
(2.4) 		64.2
(3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184688_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4851
Sequence Type Exemplar sequence
Representative Public ID Y49F6B.8 NCBI
Target Description Y49F6B.8 /REP_DB=WormBase Gene ID /WP=CE25341 /TR=Q9N4U6 /GB=AAF60734.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062157(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062157 NCBI Caenorhabditis elegans Y49F6B.8 (Y49F6B.8) mRNA, complete cds. 11/11 None
SNAP00000021096 ENSEMBL cdna:SNAP chromosome:CEL140:II:3479219:3489496:-1 11/11 None
GENEFINDER00000021112 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3474947:3480873:-1 11/11 None
Y49F6B.8 ENSEMBL cdna:known chromosome:CEL140:II:3479468:3480558:-1 gene:Y49F6B.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3479466-3480557(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y49F6B.8
Entrez Gene 190082 Entrez gene
SwissProt Q9N4U6 EMBL-EBI
WormBase CE25341 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494558 Y49F6B.8 [Caenorhabditis elegans] gb|AAF60734.1| Hypothetical protein Y49F6B.8 [Caenorhabditis elegans] 1.0E-172
blast NP_494558 Y49F6B.8 [Caenorhabditis elegans] gb|AAF60734.1| Hypothetical protein Y49F6B.8 [Caenorhabditis elegans] 1.0E-167
blast NP_494558 Y49F6B.8 [Caenorhabditis elegans] gb|AAF60734.1| Hypothetical protein Y49F6B.8 [Caenorhabditis elegans] 1.0E-164
blast NP_494557 Y49F6B.7 [Caenorhabditis elegans] gb|AAF60733.1| Hypothetical protein Y49F6B.7 [Caenorhabditis elegans] 2.0E-98
blast CAA94574 Hypothetical protein C25G4.8 [Caenorhabditis elegans] ref|NP_502382.1| C25G4.8 [Caenorhabditis elegans] 2.0E-79
blast CAA94574 Hypothetical protein C25G4.8 [Caenorhabditis elegans] ref|NP_502382.1| C25G4.8 [Caenorhabditis elegans] 2.0E-70
blast CAB00877 Hypothetical protein T16A9.3 [Caenorhabditis elegans] ref|NP_506517.2| T16A9.3 [Caenorhabditis elegans] 8.0E-70
blast CAA94574 Hypothetical protein C25G4.8 [Caenorhabditis elegans] ref|NP_502382.1| C25G4.8 [Caenorhabditis elegans] 3.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR009676 EMBL-EBI Protein of unknown function DUF1265 7.4E-32
Pfam IPR009676 EMBL-EBI Protein of unknown function DUF1265 7.4E-32
Pfam IPR009676 EMBL-EBI Protein of unknown function DUF1265 7.4E-32
Sequence
Target Sequence 
>C. ELEGANS:184688_AT
cagtgagatggtctgtctggcttctgggtacttccgtgcatttttgacggagcacaccac
agagctcaccgttgaatgctccgatgtggaggctattaacatctgtctcgtatacatggt
caccagacattacaaaaagcctgcccagctcacaccaagattggcttctgagatatcatc
tttggctgagcattggttcaccaatcctgattcggaaaagctgagaagctcgctcgaaaa
acatctttgtctggagctgcagacgaactatgaggaccttccatatgtctgcaatttgat
gatcatcgctgaggacgccaagtttcgaaacgttgaaacgtgctgcattgccactatcgt
gtcttaccatgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGTGAGATGGTCTGTCTGGCTTCT 472 207 480 Antisense
TTCTGGGTACTTCCGTGCATTTTTG 556 693 501 Antisense
GCATTTTTGACGGAGCACACCACAG 137 309 517 Antisense
CACCACAGAGCTCACCGTTGAATGC 452 201 534 Antisense
ATCTGTCTCGTATACATGGTCACCA 87 35 580 Antisense
ATTACAAAAAGCCTGCCCAGCTCAC 240 13 608 Antisense
ATTGGTTCACCAATCCTGATTCGGA 336 3 671 Antisense
GAGGACCTTCCATATGTCTGCAATT 183 405 751 Antisense
GATCATCGCTGAGGACGCCAAGTTT 217 421 780 Antisense
AAACGTGCTGCATTGCCACTATCGT 201 127 815 Antisense
TGCCACTATCGTGTCTTACCATGCA 222 587 828 Antisense
Sequence Source Affymetrix Proprietary Database