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[184672_at]

Description F54A5.3A /REP_DB=WormBase Gene ID /WP=CE20861 /TR=Q9TYT3 /GB=AAC78222.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 59      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 14.8
(-2.1)
A 		13.6
(-3)
A 		20.6
(2.5)
A 		21.2
(3)
A 		8
B 12.4
(2.8)
A 		12.5
(3)
A 		9.7
(-3)
A 		10.4
(-1.5)
A 		3
C 43
(1.2)
A 		52
(2.3)
A 		56.9
(3)
A 		11.7
(-3)
A 		45
avr 23.4
(0.7) 		26
(1.7) 		29.1
(3) 		14.4
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 8.4
(-0.5)
A 		11.9
(3)
A 		9.4
(0.5)
A 		10.3
(1.4)
A 		5.9
(-3)
A 		8.3
(-0.6)
A 		6
B 8.8
(-1.7)
A 		18.1
(2.9)
A 		10.4
(-0.9)
A 		6.2
(-3)
A 		18.3
(3)
A 		7.2
(-2.5)
A 		12
C 9.4
(-2.1)
A 		33
(3)
A 		5.4
(-3)
A 		13.2
(-1.3)
A 		8.4
(-2.3)
A 		6.8
(-2.7)
A 		28
avr 8.9
(-2.3) 		21
(3) 		8.4
(-2.6) 		9.9
(-1.9) 		10.9
(-1.5) 		7.4
(-3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 5.4
(3)
A 		2.9
(-3)
A 		5.4
(3)
A 		3.1
(-2.5)
A 		3
B 4
(3)
A 		3.1
(0)
A 		2.2
(-3)
A 		2.2
(-3)
A 		2
C 14.3
(1.1)
A 		15
(1.4)
A 		19.8
(3)
A 		2
(-3)
A 		18
avr 7.9
(1.9) 		7
(1.1) 		9.1
(3) 		2.4
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 1.6
(-3)
A 		4.2
(3)
A 		2
(-2.1)
A 		2.6
(-0.7)
A 		1.8
(-2.5)
A 		2
(-2.1)
A 		3
B 3.1
(-1.4)
A 		6
(1.6)
A 		2.5
(-2.1)
A 		1.6
(-3)
A 		7.4
(3)
A 		2.7
(-1.9)
A 		6
C 3.5
(-1.6)
A 		10.1
(3)
A 		1.4
(-3)
A 		2.6
(-2.2)
A 		2.7
(-2.1)
A 		2.3
(-2.4)
A 		9
avr 2.7
(-2.1) 		6.8
(3) 		2
(-3) 		2.3
(-2.6) 		4
(-0.5) 		2.3
(-2.6) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184672_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8
Sequence Type Exemplar sequence
Representative Public ID F54A5.3A NCBI
Target Description F54A5.3A /REP_DB=WormBase Gene ID /WP=CE20861 /TR=Q9TYT3 /GB=AAC78222.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058398(11), NM_058400(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058398 NCBI Caenorhabditis elegans F54A5.3a (F54A5.3) mRNA, complete cds. 11/11 A 
NM_058400 NCBI Caenorhabditis elegans F54A5.3d (F54A5.3) mRNA, complete cds. 11/11 None
F54A5.3a ENSEMBL cdna:known chromosome:CEL140:I:969682:973135:-1 gene:F54A5.3 11/11 A 
F54A5.3d ENSEMBL cdna:known chromosome:CEL140:I:969709:973137:-1 gene:F54A5.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000014310 7/11 Cross Hyb Matching Probes None
SNAP00000014307 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
3565745 4 2 NM_058398 184672_at (11)
NM_058400 184672_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:969681-973054(-) 100.0 100.0
Public Domain and Genome References
Ensembl F54A5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78222 Hypothetical protein F54A5.3a [Caenorhabditis elegans] ref|NP_490799.2| F54A5.3a [Caenorhabditis elegans] 1.0E-170
blast CAE61300 Hypothetical protein CBG05128 [Caenorhabditis briggsae] 1.0E-83
blast AAL06026 Hypothetical protein F54A5.3d [Caenorhabditis elegans] ref|NP_490801.1| F54A5.3d [Caenorhabditis elegans] 7.0E-34
blast AAC78222 Hypothetical protein F54A5.3a [Caenorhabditis elegans] ref|NP_490799.2| F54A5.3a [Caenorhabditis elegans] 5.0E-31
blast AAC78223 Hypothetical protein F54A5.3b [Caenorhabditis elegans] ref|NP_490800.1| F54A5.3b [Caenorhabditis elegans] 2.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 1.4E-29
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 2.8E-6
Sequence
Target Sequence 
>C. ELEGANS:184672_AT
atcgtcgatgccacgtggtgttcatcgaggatgtgcacaagctcatcgacgtccttacca
ctgcgatcaatgtgaacacttttgatgcacaggcgaatgcttcaacttctaacgatggat
ttacggtgccggcgccgccgatgagacatcgaagttccctgcatcgacagtcatttgtgt
cgaattgccgagcacctactgtaactgaggatgttgtggggaaagtttggtatcatggaa
atttatccagagaggatgcacaggctttgttgaagacggaaggcgattttttggttcgac
aaagcgaccacacgcccggcaagtacgtgctgagtggccgaaccgctgaaaacgagcaca
aacatctcatcctactggacaatcacaacgtaagaccctccccccccccccccgccaaac
cttcaaacctctcaaaattccagagagtccgaacccgcgatcgcacgttcagcaacatct
ccgagctcatcgactatcatgtgaacaatgggatggccgtaagatcagagggtcgggatc
gcgagacgtcgttgaatctgatccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGTCGATGCCACGTGGTGTTCAT 605 35 416 Antisense
GGATGTGCACAAGCTCATCGACGTC 151 515 444 Antisense
CGTCCTTACCACTGCGATCAATGTG 400 243 465 Antisense
CAGGCGAATGCTTCAACTTCTAACG 483 209 505 Antisense
TAACGATGGATTTACGGTGCCGGCG 258 639 525 Antisense
AGACATCGAAGTTCCCTGCATCGAC 525 71 559 Antisense
GAATTGCCGAGCACCTACTGTAACT 566 323 597 Antisense
CGCCCGGCAAGTACGTGCTGAGTGG 628 255 728 Antisense
TGAGTGGCCGAACCGCTGAAAACGA 62 567 746 Antisense
CGAGCACAAACATCTCATCCTACTG 165 251 768 Antisense
GCGAGACGTCGTTGAATCTGATCCG 151 291 956 Antisense
Sequence Source Affymetrix Proprietary Database