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[184668_s_at]

Description F54A5.3C /REP_DB=WormBase Gene ID /WP=CE20863 /TR=Q9TYT1 /GB=AAC78224.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 35      4grp_total > 07      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 65
(3)
A 		8.3
(-3)
A 		21.3
(-1.6)
A 		52.2
(1.6)
A 		57
B 52.3
(3)
A 		13.4
(-3)
A 		38.1
(0.8)
A 		42
(1.4)
A 		39
C 10
(-3)
A 		23.9
(-0.1)
A 		15.1
(-1.9)
A 		39.1
(3)
A 		29
avr 42.4
(2.6) 		15.2
(-3) 		24.8
(-1) 		44.4
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 20.7
(-1.3)
A 		4.3
(-3)
A 		64.8
(3)
A 		8.5
(-2.5)
A 		3.9
(-3)
A 		34.5
(0)
A 		61
B 35.2
(3)
A 		10.3
(-1.7)
A 		3.5
(-3)
A 		19.2
(-0)
A 		7.3
(-2.3)
A 		32.9
(2.6)
A 		32
C 53.8
(0.8)
A 		82.1
(3)
A 		10.2
(-2.6)
A 		5.2
(-3)
A 		8.9
(-2.7)
A 		7.8
(-2.8)
A 		77
avr 36.6
(3) 		32.2
(2.1) 		26.2
(0.9) 		11
(-2.1) 		6.7
(-3) 		25.1
(0.7) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 23.7
(3)
A 		1.8
(-3)
A 		5.6
(-2)
A 		7.6
(-1.4)
A 		22
B 16.7
(3)
A 		3.3
(-3)
A 		8.8
(-0.5)
A 		8.9
(-0.5)
A 		13
C 3.3
(-3)
A 		6.9
(3)
A 		5.3
(0.3)
A 		6.6
(2.5)
A 		4
avr 14.6
(3) 		4
(-3) 		6.6
(-1.5) 		7.7
(-0.9) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 3.8
(-1.8)
A 		1.5
(-2.9)
A 		14.1
(3)
A 		2.2
(-2.5)
A 		1.2
(-3)
A 		8.4
(0.3)
A 		13
B 12.3
(3)
A 		3.4
(-1.7)
A 		0.9
(-3)
A 		5.1
(-0.8)
A 		3
(-1.9)
A 		12.3
(3)
A 		11
C 19.9
(1.7)
A 		25
(3)
A 		2.6
(-2.6)
A 		1
(-3)
A 		2.9
(-2.5)
A 		2.6
(-2.6)
A 		24
avr 12
(3) 		10
(1.8) 		5.9
(-0.8) 		2.8
(-2.8) 		2.4
(-3) 		7.8
(0.4) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184668_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13
Sequence Type Exemplar sequence
Representative Public ID F54A5.3C NCBI
Target Description F54A5.3C /REP_DB=WormBase Gene ID /WP=CE20863 /TR=Q9TYT1 /GB=AAC78224.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058398(11), NM_058400(11), NM_058399(11), NM_058401(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058398 NCBI Caenorhabditis elegans F54A5.3a (F54A5.3) mRNA, complete cds. 11/11 A 
NM_058400 NCBI Caenorhabditis elegans F54A5.3d (F54A5.3) mRNA, complete cds. 11/11 A 
NM_058399 NCBI Caenorhabditis elegans F54A5.3b (F54A5.3) mRNA, complete cds. 11/11 A 
NM_058401 NCBI Caenorhabditis elegans F54A5.3c (F54A5.3) mRNA, complete cds. 11/11 None
F54A5.3a ENSEMBL cdna:known chromosome:CEL140:I:969682:973135:-1 gene:F54A5.3 11/11 A 
F54A5.3d ENSEMBL cdna:known chromosome:CEL140:I:969709:973137:-1 gene:F54A5.3 11/11 A 
F54A5.3b ENSEMBL cdna:known chromosome:CEL140:I:971901:973114:-1 gene:F54A5.3 11/11 A 
F54A5.3c ENSEMBL cdna:known chromosome:CEL140:I:971952:973139:-1 gene:F54A5.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000014310 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:972846-973054(-) 100.0 100.0
Public Domain and Genome References
Ensembl F54A5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78222 Hypothetical protein F54A5.3a [Caenorhabditis elegans] ref|NP_490799.2| F54A5.3a [Caenorhabditis elegans] 1.0E-170
blast CAE61300 Hypothetical protein CBG05128 [Caenorhabditis briggsae] 1.0E-83
blast AAC78223 Hypothetical protein F54A5.3b [Caenorhabditis elegans] ref|NP_490800.1| F54A5.3b [Caenorhabditis elegans] 1.0E-37
blast AAL06026 Hypothetical protein F54A5.3d [Caenorhabditis elegans] ref|NP_490801.1| F54A5.3d [Caenorhabditis elegans] 7.0E-34
blast AAC78222 Hypothetical protein F54A5.3a [Caenorhabditis elegans] ref|NP_490799.2| F54A5.3a [Caenorhabditis elegans] 5.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 1.4E-29
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 2.8E-6
Sequence
Target Sequence 
>C. ELEGANS:184668_S_AT
atgctcaacgtggagccctcatttgcagaggagctgcggagcagcggggtttctctgtcg
gctacttacctcggaagtgttccagtagtcgagtcaattaacgtgatggtttccgagatg
agagttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTCAACGTGGAGCCCTCATTTG 663 41 13 Antisense
TCAACGTGGAGCCCTCATTTGCAGA 406 631 17 Antisense
GAGCCCTCATTTGCAGAGGAGCTGC 191 387 25 Antisense
TTTGCAGAGGAGCTGCGGAGCAGCG 280 667 34 Antisense
GAGCTGCGGAGCAGCGGGGTTTCTC 183 385 43 Antisense
TCGGCTACTTACCTCGGAAGTGTTC 175 607 70 Antisense
GCTACTTACCTCGGAAGTGTTCCAG 43 301 73 Antisense
GGAAGTGTTCCAGTAGTCGAGTCAA 121 533 85 Antisense
GTGTTCCAGTAGTCGAGTCAATTAA 14 485 89 Antisense
GAGTCAATTAACGTGATGGTTTCCG 485 399 103 Antisense
GATGGTTTCCGAGATGAGAGTTCAA 320 407 117 Antisense
Sequence Source Affymetrix Proprietary Database