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[184657_at]

Description H20J04.3 /REP_DB=WormBase Gene ID /WP=CE23813 /TR=Q9N5M0 /GB=AAF39889.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 71      4grp_total > 18      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 1.3
(-3)
A 		5.5
(-1.3)
A 		2.2
(-2.6)
A 		16.4
(3)
A 		15
B 1.3
(-3)
A 		3.9
(3)
A 		3.1
(1.2)
A 		3.3
(1.6)
A 		3
C 1.4
(-2.2)
A 		1.6
(-1.6)
A 		1.1
(-3)
A 		3.3
(3)
A 		2
avr 1.3
(-3) 		3.7
(-0.8) 		2.1
(-2.3) 		7.7
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 1
(-3)
A 		1.5
(-2.2)
A 		3.7
(1.4)
A 		4.7
(3)
A 		3.8
(1.5)
A 		2
(-1.4)
A 		4
B 1.2
(-2.8)
A 		1.1
(-3)
A 		1.2
(-2.8)
A 		4.6
(3)
A 		1.1
(-3)
A 		1.5
(-2.3)
A 		4
C 1.6
(-1.6)
A 		1.5
(-1.9)
A 		3.2
(3)
A 		1.9
(-0.7)
A 		2.4
(0.7)
A 		1.1
(-3)
A 		2
avr 1.3
(-3) 		1.4
(-2.8) 		2.7
(0.5) 		3.7
(3) 		2.4
(-0.3) 		1.5
(-2.5) 		2
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 0.5
(-3)
A 		1.2
(-0.8)
A 		0.6
(-2.7)
A 		2.4
(3)
A 		2
B 0.4
(-3)
A 		1
(3)
A 		0.7
(-0)
A 		0.7
(-0)
A 		1
C 0.5
(0)
A 		0.5
(0)
A 		0.4
(-3)
A 		0.6
(3)
A 		0
avr 0.5
(-3) 		0.9
(0.4) 		0.6
(-2.1) 		1.2
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1
1 2 3 4 5 6 range
A 0.2
(-3)
A 		0.6
(-0.6)
A 		0.8
(0.6)
A 		1.2
(3)
A 		1.1
(2.4)
A 		0.5
(-1.2)
A 		1
B 0.4
(-2.3)
A 		0.4
(-2.3)
A 		0.3
(-3)
A 		1.2
(3)
A 		0.5
(-1.7)
A 		0.6
(-1)
A 		1
C 0.6
(-0)
A 		0.5
(-1.5)
A 		0.8
(3)
A 		0.4
(-3)
A 		0.8
(3)
A 		0.4
(-3)
A 		0
avr 0.4
(-3) 		0.5
(-1.8) 		0.6
(-0.6) 		0.9
(3) 		0.8
(1.8) 		0.5
(-1.8) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184657_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4262
Sequence Type Exemplar sequence
Representative Public ID H20J04.3 NCBI
Target Description H20J04.3 /REP_DB=WormBase Gene ID /WP=CE23813 /TR=Q9N5M0 /GB=AAF39889.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062365(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062365 NCBI Caenorhabditis elegans H20J04.3 (H20J04.3) mRNA, complete cds. 11/11 None
SNAP00000017488 ENSEMBL cdna:SNAP chromosome:CEL140:II:4329025:4350547:1 10/11 None
GENEFINDER00000017494 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4328677:4338418:1 11/11 None
H20J04.3 ENSEMBL cdna:known chromosome:CEL140:II:4335701:4338418:1 gene:H20J04.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4335699-4338417(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H20J04.3
Entrez Gene 186755 Entrez gene
SwissProt Q9N5M0 EMBL-EBI
WormBase CE23813 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39889 Hypothetical protein H20J04.3 [Caenorhabditis elegans] ref|NP_494766.1| H20J04.3 [Caenorhabditis elegans] 0.0
blast AAF39888 Hypothetical protein H20J04.2 [Caenorhabditis elegans] ref|NP_494767.1| H20J04.2 [Caenorhabditis elegans] 0.0
blast CAE60124 Hypothetical protein CBG03667 [Caenorhabditis briggsae] 0.0
blast AAF39890 Hypothetical protein H20J04.4 [Caenorhabditis elegans] ref|NP_494765.2| H20J04.4 [Caenorhabditis elegans] 0.0
blast AAF39889 Hypothetical protein H20J04.3 [Caenorhabditis elegans] ref|NP_494766.1| H20J04.3 [Caenorhabditis elegans] 1.0E-180
blast CAE60123 Hypothetical protein CBG03666 [Caenorhabditis briggsae] 1.0E-129
Protein Domains
Database ID Description E-Value
Pfam IPR001965 EMBL-EBI Zn-finger-like, PHD finger 1.5E-10
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.8E-31
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.2E-75
Pfam IPR001487 EMBL-EBI Bromodomain 2.3E-30
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.8E-31
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.6E-61
Sequence
Target Sequence 
>C. ELEGANS:184657_AT
aaagattgctgagccatgttttggaaaaaatctaattcttgaagtgagaaggcatccgtg
tctttatgatgttcgtgatccaaaatatcgtcatggagactgtcgtactcaagcatgggg
aatgattattgataaactacgatatcctggaacagttccgtcgatttataaacaatggaa
gaagcatcgagatcgatatgttagggagaagaggaggctgaggaatttgggtgatccgaa
tgttcaagatgtgtcaacttgggagatgtatgatgatatggcttggatagatcagcattt
ggatgaacagcaactatctcgatgtgctcgaagtctgaaacgagggggaaacaatgacgg
aggaaatcaggatgaaatgtcagattatggagattacgatgatgacataaactacgtgat
gatggctgaaaagcgaccaaacaacggtgatgtacttctcgacggagattctgcattctc
tgcatcaattgtatcggatctaaggacacttggagaagaagcacgaataattgcaaagca
gcagattatgatacttttggaatccgctaaacct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGATTGCTGAGCCATGTTTTGGA 273 123 348 Antisense
GTGAGAAGGCATCCGTGTCTTTATG 102 483 391 Antisense
GTGTCTTTATGATGTTCGTGATCCA 703 485 405 Antisense
AGACTGTCGTACTCAAGCATGGGGA 296 65 444 Antisense
TACGATATCCTGGAACAGTTCCGTC 386 647 485 Antisense
GGAATTTGGGTGATCCGAATGTTCA 296 515 569 Antisense
ACAGCAACTATCTCGATGTGCTCGA 245 109 654 Antisense
CAACGGTGATGTACTTCTCGACGGA 176 187 789 Antisense
GACGGAGATTCTGCATTCTCTGCAT 280 377 808 Antisense
GCATTCTCTGCATCAATTGTATCGG 600 307 820 Antisense
GATACTTTTGGAATCCGCTAAACCT 418 303 897 Antisense
Sequence Source Affymetrix Proprietary Database