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[184655_at]

Description T21E12.5 /REP_DB=WormBase Gene ID /WP=CE13886 /TR=P91485 /GB=AAK21471.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 04      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 47.6
(-0.4)
A 		8.3
(-3)
A 		10.5
(-2.9)
A 		97.7
(3)
A 		89
B 43.8
(0.7)
A 		23.3
(-1.7)
A 		11.3
(-3)
A 		64.7
(3)
A 		53
C 14.4
(-2.7)
A 		10.2
(-3)
A 		11.5
(-2.9)
A 		105.1
(3)
A 		95
avr 35.3
(-1.1) 		13.9
(-2.8) 		11.1
(-3) 		89.2
(3) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 41.8
(-2)
A 		29.1
(-3)
A 		73.6
(0.5)
A 		41.1
(-2.1)
A 		106.2
(3)
A 		76.3
(0.7)
A 		77
B 56.9
(-1)
A 		61.3
(-0.7)
A 		55.5
(-1.1)
A 		29.3
(-3)
A 		112.8
(3)
A 		111.7
(2.9)
A 		84
C 27.1
(-1.6)
A 		98.8
(3)
A 		6.1
(-3)
A 		79.5
(1.7)
A 		78.5
(1.7)
A 		98.9
(3)
A 		93
avr 41.9
(-3) 		63.1
(-0.8) 		45.1
(-2.7) 		50
(-2.2) 		99.2
(3) 		95.6
(2.6) 		57
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 17.3
(3)
A 		1.8
(-3)
A 		2.7
(-2.7)
A 		14.3
(1.8)
A 		16
B 14
(3)
A 		5.8
(-1.3)
A 		2.6
(-3)
A 		13.8
(2.9)
A 		11
C 4.8
(-2.2)
A 		2.9
(-3)
A 		4
(-2.6)
A 		17.7
(3)
A 		15
avr 12
(1.4) 		3.5
(-2.8) 		3.1
(-3) 		15.3
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 7.7
(-3)
A 		10.4
(-2.3)
A 		16
(-1)
A 		10.4
(-2.3)
A 		32
(3)
A 		18.6
(-0.3)
A 		24
B 19.8
(-1.1)
A 		20.4
(-1)
A 		13.5
(-2.1)
A 		7.8
(-3)
A 		45.8
(3)
A 		41.7
(2.4)
A 		38
C 10
(-1.4)
A 		30.2
(2.4)
A 		1.6
(-3)
A 		16
(-0.3)
A 		25.4
(1.5)
A 		33.1
(3)
A 		32
avr 12.5
(-2.5) 		20.3
(-0.5) 		10.4
(-3) 		11.4
(-2.8) 		34.4
(3) 		31.1
(2.2) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184655_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1215
Sequence Type Exemplar sequence
Representative Public ID T21E12.5 NCBI
Target Description T21E12.5 /REP_DB=WormBase Gene ID /WP=CE13886 /TR=P91485 /GB=AAK21471.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058965(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058965 NCBI Caenorhabditis elegans T21E12.5 (T21E12.5) mRNA, complete cds. 11/11 None
SNAP00000041458 ENSEMBL cdna:SNAP chromosome:CEL140:I:4412296:4413222:-1 11/11 None
GENEFINDER00000041461 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4412296:4413222:-1 11/11 None
T21E12.5 ENSEMBL cdna:known chromosome:CEL140:I:4412296:4413222:-1 gene:T21E12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4412295-4413222(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T21E12.5
Entrez Gene 188701 Entrez gene
SwissProt P91485 EMBL-EBI
WormBase CE13886 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK21471 Hypothetical protein T21E12.5 [Caenorhabditis elegans] ref|NP_491366.1| T21E12.5 [Caenorhabditis elegans] 1.0E-102
blast CAE66828 Hypothetical protein CBG12198 [Caenorhabditis briggsae] 3.0E-47
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491366.1 2 43-65,85-104
T21E12.5 2 43-65,85-104
SNAP00000041458 2 43-65,85-104
GENEFINDER00000041461 2 43-65,85-104
Sequence
Target Sequence 
>C. ELEGANS:184655_AT
cccagccaggtgatgttcctttgaatattaaaaaagttgacctgattgatggactaggat
ttcaatggaataattcgattgttcgctctacagtctgttctatttttaaagtaataataa
aagtggtgttccttatgatttgtgtcagtgcatgttgcgttttgctgtggcttgaagcaa
tcacaattgctaagacaactccaatccacacattggaagaaatgcaacaagttgaagacg
aagaagctctgatgcatataaaatgcttcatccttttattgtggtcattttcagtttaca
ctcttatcacagaaatgacaaagatcatgctgctcaatgagaaaattcgaattctcgaat
cagtgagacatcgttcgaaaatggagtcaactctttcacttattcgacaaaaatcatatt
tcgacgcgtacgcctctgattttattattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCAGCCAGGTGATGTTCCTTTGAA 158 259 17 Antisense
GGAATAATTCGATTGTTCGCTCTAC 542 527 83 Antisense
GTTCGCTCTACAGTCTGTTCTATTT 170 443 97 Antisense
TGATTTGTGTCAGTGCATGTTGCGT 521 563 152 Antisense
CATGTTGCGTTTTGCTGTGGCTTGA 150 211 167 Antisense
TTGCTAAGACAACTCCAATCCACAC 666 703 203 Antisense
AATGCTTCATCCTTTTATTGTGGTC 687 165 278 Antisense
GTCATTTTCAGTTTACACTCTTATC 467 467 300 Antisense
GAATCAGTGAGACATCGTTCGAAAA 568 327 373 Antisense
GGAGTCAACTCTTTCACTTATTCGA 178 517 399 Antisense
GCGTACGCCTCTGATTTTATTATTC 124 289 442 Antisense
Sequence Source Affymetrix Proprietary Database