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[184649_at]

Description F33D11.5 /REP_DB=WormBase Gene ID /WP=CE09895 /TR=O44773 /GB=AAB96694.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 25      4grp_total > 13      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 69.7
(3)
P 		35.9
(-1.5)
A 		24.6
(-3)
A 		57.4
(1.4)
A 		45
B 71.2
(-0.3)
A 		57.2
(-1.8)
A 		45.7
(-3)
A 		102.8
(3)
M 		57
C 66.1
(-0.2)
A 		33.6
(-3)
A 		34.9
(-2.9)
A 		103.4
(3)
P 		70
avr 69
(0.9) 		42.2
(-2.2) 		35.1
(-3) 		87.9
(3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 66
(1.7)
A 		75.9
(3)
P 		59.5
(0.9)
P 		56.5
(0.5)
A 		29.1
(-3)
A 		29.2
(-3)
A 		47
B 59.7
(1.3)
P 		79.5
(3)
A 		44.2
(0)
P 		7.9
(-3)
A 		34.1
(-0.8)
A 		45.7
(0.2)
A 		72
C 55.4
(-0.1)
M 		84.7
(3)
P 		28.2
(-3)
P 		69.3
(1.4)
A 		29
(-2.9)
A 		41.6
(-1.6)
A 		57
avr 60.4
(0.6) 		80
(3) 		44
(-1.4) 		44.6
(-1.3) 		30.7
(-3) 		38.8
(-2) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 25.4
(3)
P 		7.8
(-2.6)
A 		6.4
(-3)
A 		8.4
(-2.4)
A 		19
B 22.7
(3)
A 		14.1
(-1.3)
A 		10.6
(-3)
A 		21.9
(2.6)
M 		12
C 21.9
(3)
A 		9.7
(-3)
A 		12.1
(-1.8)
A 		17.4
(0.8)
P 		12
avr 23.3
(3) 		10.5
(-2.6) 		9.7
(-3) 		15.9
(-0.3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 12.2
(-1.5)
A 		27
(3)
P 		13
(-1.2)
P 		14.3
(-0.8)
A 		8.8
(-2.5)
A 		7.1
(-3)
A 		20
B 20.8
(1.6)
P 		26.5
(3)
A 		10.7
(-0.9)
P 		2.1
(-3)
A 		13.9
(-0.1)
A 		17
(0.7)
A 		24
C 20.5
(1.3)
M 		25.9
(3)
P 		7.2
(-3)
P 		13.9
(-0.9)
A 		9.4
(-2.3)
A 		13.9
(-0.9)
A 		19
avr 17.8
(-0.2) 		26.5
(3) 		10.3
(-2.9) 		10.1
(-3) 		10.7
(-2.8) 		12.7
(-2) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184649_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.412
Sequence Type Exemplar sequence
Representative Public ID F33D11.5 NCBI
Target Description F33D11.5 /REP_DB=WormBase Gene ID /WP=CE09895 /TR=O44773 /GB=AAB96694.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059291(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059291 NCBI Caenorhabditis elegans F33D11.5 (F33D11.5) mRNA, complete cds. 11/11 None
SNAP00000028948 ENSEMBL cdna:SNAP chromosome:CEL140:I:5842780:5845351:-1 11/11 None
GENEFINDER00000028966 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5842780:5845342:-1 11/11 None
F33D11.5 ENSEMBL cdna:known chromosome:CEL140:I:5842780:5845528:-1 gene:F33D11.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5842779-5845528(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F33D11.5
Entrez Gene 185227 Entrez gene
SwissProt O44773 EMBL-EBI
WormBase CE09895 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB96694 Hypothetical protein F33D11.5 [Caenorhabditis elegans] ref|NP_491692.1| F33D11.5 [Caenorhabditis elegans] 0.0
blast CAE60449 Hypothetical protein CBG04057 [Caenorhabditis briggsae] 0.0
blast AAM51529 Twik family of potassium channels protein 17, isoform a [Caenorhabditis elegans] ref|NP_001024462.1| TWiK family of potassium channels family member (twk-17) [Caenorhabditis elegans] 2.0E-40
blast AAM51529 Twik family of potassium channels protein 17, isoform a [Caenorhabditis elegans] ref|NP_001024462.1| TWiK family of potassium channels family member (twk-17) [Caenorhabditis elegans] 3.0E-39
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491692.1 4 26-48,80-102,197-219,247-269
F33D11.5 4 26-48,80-102,197-219,247-269
SNAP00000028948 6 5-27,110-132,137-159,240-262,266-283,296-318
GENEFINDER00000028966 6 6-25,107-129,134-156,237-259,263-280,293-315
Sequence
Target Sequence 
>C. ELEGANS:184649_AT
gaactcaatcatattctcactattatttatatttttgtgttcgtcacgtgtggagtagca
gttctctcaatgagtgccgaattggcggcattggaactcaaagagctatttatgaaaatt
cattattttggacgtcgaattaatttcaaaagaagacagccaaaagaacaaatggatgtt
caagtgaaagaattgttgaaaataattgaagaaataagaaaacgatatccagaaaaagag
aaaattaccaccttggacattttggagtatatgcacgaaaataccgcaaatgacaaactg
aatgagcgtcgtgatacaattgcatttatgcctcaaacaatggaagttattaaatttgcc
gatgagcaagatttggaggaaagaagtttttctcgtcacgaagatgttgcttcacttggt
ggaaaggcttcaacaaaactagcttctgtggtcgacgtgatgagcttctcacctgaggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTCAATCATATTCTCACTATTA 692 349 748 Antisense
ATATTTTTGTGTTCGTCACGTGTGG 596 19 776 Antisense
TCGTCACGTGTGGAGTAGCAGTTCT 346 605 788 Antisense
GTAGCAGTTCTCTCAATGAGTGCCG 441 457 802 Antisense
CCGAATTGGCGGCATTGGAACTCAA 674 261 824 Antisense
ATTACCACCTTGGACATTTTGGAGT 325 11 991 Antisense
GAGCGTCGTGATACAATTGCATTTA 337 387 1051 Antisense
AAGAAGTTTTTCTCGTCACGAAGAT 417 129 1128 Antisense
AAGATGTTGCTTCACTTGGTGGAAA 260 155 1148 Antisense
AAAACTAGCTTCTGTGGTCGACGTG 173 127 1182 Antisense
CGTGATGAGCTTCTCACCTGAGGAA 448 245 1203 Antisense
Sequence Source Affymetrix Proprietary Database