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[184629_at]

Description Y74E4A.1 /REP_DB=WormBase Gene ID /WP=CE24664 /TR=Q9N4D4 /GB=AAF36053.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 80      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 54      6grp_total > 82      4grp_total > 49      6grp_high1_sub > 02_03_04_05      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1859
1 2 3 4 range
A 137.8
(-3)
P 		1749.6
(3)
P 		1726.4
(2.9)
P 		198.6
(-2.8)
P 		1612
B 148.2
(-3)
P 		1815.7
(3)
P 		1750.3
(2.8)
P 		223.9
(-2.7)
P 		1668
C 142.1
(-3)
P 		1997.1
(3)
P 		1717.3
(2.1)
P 		261.8
(-2.6)
P 		1855
avr 142.7
(-3) 		1854.1
(3) 		1731.3
(2.6) 		228.1
(-2.7) 		1711

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 972
1 2 3 4 5 6 range
A 444.9
(-3)
P 		1417.1
(3)
P 		886.3
(-0.3)
P 		1328.2
(2.5)
P 		1141
(1.3)
P 		726.3
(-1.3)
P 		972
B 461.1
(-3)
P 		1197.4
(2.6)
P 		951.9
(0.8)
P 		1234.1
(2.9)
P 		1244.2
(3)
P 		652
(-1.5)
P 		783
C 520
(-3)
P 		1220.1
(1.8)
P 		1015.2
(0.4)
P 		1310.3
(2.5)
P 		1389.8
(3)
P 		588.1
(-2.5)
P 		870
avr 475.3
(-3) 		1278.2
(2.9) 		951.1
(0.5) 		1290.9
(3) 		1258.3
(2.8) 		655.5
(-1.7) 		816
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 569
1 2 3 4 range
A 50.2
(-2.7)
P 		379.8
(2)
P 		451.3
(3)
P 		29.1
(-3)
P 		422
B 47.3
(-3)
P 		448.4
(3)
P 		405.6
(2.4)
P 		47.7
(-3)
P 		401
C 47
(-3)
P 		574.2
(2.7)
P 		598.5
(3)
P 		44.1
(-3)
P 		554
avr 48.2
(-2.9) 		467.5
(2.8) 		485.1
(3) 		40.3
(-3) 		445

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 424
1 2 3 4 5 6 range
A 82
(-3)
P 		504
(3)
P 		193
(-1.4)
P 		336.9
(0.6)
P 		344.3
(0.7)
P 		176.8
(-1.7)
P 		422
B 160.4
(-3)
P 		398.6
(1.1)
P 		231.1
(-1.8)
P 		326.9
(-0.1)
P 		505.5
(3)
P 		243.1
(-1.6)
P 		345
C 192.7
(-3)
P 		372.3
(1.2)
P 		258.4
(-1.5)
P 		263.5
(-1.3)
P 		449.7
(3)
P 		196.9
(-2.9)
P 		257
avr 145
(-3) 		425
(2.8) 		227.5
(-1.3) 		309.1
(0.4) 		433.2
(3) 		205.6
(-1.7) 		288
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184629_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4607
Sequence Type Exemplar sequence
Representative Public ID Y74E4A.1 NCBI
Target Description Y74E4A.1 /REP_DB=WormBase Gene ID /WP=CE24664 /TR=Q9N4D4 /GB=AAF36053.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170980(11), NM_171848(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170980 NCBI Caenorhabditis elegans Y74E4A.1b (Y74E4A.1) mRNA, complete cds. 11/11 None
NM_171848 NCBI Caenorhabditis elegans Y74E4A.1a (Y74E4A.1) mRNA, complete cds. 11/11 None
Y74E4A.1b ENSEMBL cdna:known chromosome:CEL140:II:4649081:4653259:1 gene:Y74E4A.1 11/11 None
Y74E4A.1a ENSEMBL cdna:known chromosome:CEL140:II:4649165:4653243:1 gene:Y74E4A.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043909 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4650681-4653152(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y74E4A.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL77194 Hypothetical protein Y74E4A.1b [Caenorhabditis elegans] ref|NP_740977.1| Y74E4A.1b [Caenorhabditis elegans] 0.0
blast AAL77193 Hypothetical protein Y74E4A.1a [Caenorhabditis elegans] ref|NP_740978.1| Y74E4A.1a [Caenorhabditis elegans] 0.0
blast CAE67844 Hypothetical protein CBG13431 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 8.2E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_740977.1 4 265-287,294-312,327-349,405-427
NP_740978.1 4 265-287,294-312,327-349,402-424
Y74E4A.1b 4 265-287,294-312,327-349,405-427
Y74E4A.1a 4 265-287,294-312,327-349,402-424
Sequence
Target Sequence 
>C. ELEGANS:184629_AT
gagccgaacgtctcgaagtgtgtgttggagtagtccgcaagtcatcaacccttgccgttg
aactcactctcccggttctcatttctgcattgattcttttgttggctccattctttggaa
agttcaaccagcaaatttatgtgaaaatgttcgctcttttgcttcaattcctgagcttcc
aattcttggcagagaagactccacaacttggatttggagacaatattccgaagatttacg
tcttctacgcgttcacaatcggaatgactgttctcagtcttattgcaactgtaatgatct
ctgcaatgagtcgagtaaagagaaaggttccaccagcacacagatatacccttcttgctt
cacttcttaatgccaatttgtgttgtggttctgaagaggaaccaactactgatgggactt
cttcaaaggacgcaacatcggattggcttcaagttcattcggctctcaacaatctctcat
caattcttctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCCGAACGTCTCGAAGTGTGTGT 156 383 731 Antisense
TGGAGTAGTCCGCAAGTCATCAACC 502 549 756 Antisense
AGTCATCAACCCTTGCCGTTGAACT 654 61 770 Antisense
GCATTGATTCTTTTGTTGGCTCCAT 304 307 817 Antisense
AAAATGTTCGCTCTTTTGCTTCAAT 374 133 874 Antisense
TTGCTTCAATTCCTGAGCTTCCAAT 468 701 889 Antisense
ATTTACGTCTTCTACGCGTTCACAA 407 15 964 Antisense
CACACAGATATACCCTTCTTGCTTC 485 193 1067 Antisense
CTTCTTGCTTCACTTCTTAATGCCA 526 221 1081 Antisense
GGACGCAACATCGGATTGGCTTCAA 474 523 1158 Antisense
CAACAATCTCTCATCAATTCTTCTC 573 189 1197 Antisense
Sequence Source Affymetrix Proprietary Database